iiinova — where your digital self keeps evolving

Maxim Greenberg @maxvcg.bsky.social · 15/10/2026

Guys, they are obviously vaults

Maxim Greenberg @maxvcg.bsky.social · 09/10/2026

It was such a thrill to have Haifeng Wang from Tsinghua University (one of the most sophisticated scientists working on CRISPR technologies) sit in on our lab meeting! We got such great input and ideas! #EpigenomeEditing ✏️🧬

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Maxim Greenberg @maxvcg.bsky.social · 24/09/2026

So proud of Angelique’s work, which culminated in a beautiful thesis, and a lively defense. Thanks so much to jury members @chribue.bsky.social @robklose.bsky.social @pablonavarrolab.bsky.social @danielholoch.bsky.social and Valerie Mezger for a scientifically enriching day!

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Maxim Greenberg @maxvcg.bsky.social · 15/09/2026

Very happy to announce that @emboreports.org will be sponsoring a cash prize for the best poster at Ctrl+Epi+Edit ✒️🧬! Still time to register and submit an abstract (speaking slots still open also), but it's filling up, so act fast! Thanks for the support 🙏 ctrlepiedit.sciencesconf.org

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Maxim Greenberg @maxvcg.bsky.social · 04/09/2026

I'm from DC and my parents are from SF and LA, so that pretty much validates this exercise! mydialect.us/quiz-standar...

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Maxim Greenberg @maxvcg.bsky.social · 03/09/2026

Obligated to share this human-made poster (by the Greenberg Lab's own Julie Pauliat) advertising Angélique David's upcoming defense 🤩 Plenty of easter eggs as well! Also, that monkey looks very familiar... almost like we go to the same barber...

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Maxim Greenberg @maxvcg.bsky.social · 01/09/2026

Tis the (PhD defense) season @ijmonod.bsky.social 👨‍🎓 👩‍🎓 ! I saved the best for last 😉

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Maxim Greenberg @maxvcg.bsky.social · 17/08/2026

Very excited to host @chribue.bsky.social ( @maxperutzlabs.ac.at ) for double-duty @ijmonod.bsky.social ! Thesis jury on Thursday and a seminar on Friday (with some champagne in between 😜) If you're around Paris on Sept 25, don't hesitate to come listen!

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Maxim Greenberg @maxvcg.bsky.social · 13/08/2026

I have to say, it warmed my heart to see Parisians congregate (in the dog days of August, no less) for a celestial event like it was Woodstock. 🌍 🌓🌞@ishier.bsky.social @arabette.bsky.social

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Maxim Greenberg @maxvcg.bsky.social · 27/06/2026

How about the mayor of this city I live in sob 😭

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Maxim Greenberg @maxvcg.bsky.social · 25/06/2026

Woke up remembering these old Occupy Wall Street protests, and it clicked why this QED 1% branding is so, so, so very awful

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Maxim Greenberg @maxvcg.bsky.social · 20/06/2026

Got some squatters this summer….

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

So we don’t think 5mC is “activating” enhancers in most cases, but we did find evidence that methyl-binding TFs might play a role in decommissioning (12/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

We also took advantage of the genetics in the EpiLC system. We reasoned that if active enhancers required 5mC, they would lose activity in the Dnmt KO. We could find examples, but they were rare (11/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

We noted the active methylated enhancers have the highest 5hmC signal. This indicates active demethylation rather than a requirement for 5mC (10/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

Active enhancers are generally anti-correlated with 5mC, so we were motivated to dig into this. First, we developed hydroxymethyl-ATAC (hM-ATAC) to determine how much signal was 5mC vs 5hmC (9/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

Now we could sort our ATAC peaks into different enhancer classes. We noted that: 1) H3K4me1, per se, is not really anti-correlated with 5mC (surprise), 2) primed enhancers have higher 5mC than active enhancers (not a surprise) and 3) there are still a lot of active methylated enhancers 🤔 (8/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

Next, we implemented M-CUT&Tag to see how chromatin marks relate to 5mC levels. We did the classical marks of H3K4me1 (primed/active enhancers) and H3K27ac (active) (7/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

Side note: we also devised a really nice way to visualize the data with a genome browser track (h/t @jrichardalbert.bsky.social) where we pile up the reads under our ATAC peaks and really see DNA methylation patterns emerge. As a 5mC biologist, I love this 🤩 (6/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

What about the mutants? The Dnmt KO ATAC landscape was broadly misregulated. In the Tet KO, we could see an increase in 5mC (indicating Tet activity), but only subtle effects on accessibility (5/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

Opening peaks (Cluster 1) exhibited less 5mC than closing peaks (C5). Not a surprise, but two things we noted: 1) signal from whole-genome data is always higher, which shows how M-ATAC brings unique info 2) even open peaks have a subset with high 5mC levels (4/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

From its onset, our lab has been using the ESC-EpiLC transition to study the requirements of 5mC, as it is resilient to mutations in Dnmt or Tet machinery. Priscillia and I thought this would be an excellent system to employ her tools to study enhancer dynamics (3/13)

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Maxim Greenberg @maxvcg.bsky.social · 15/06/2026

During her post-doc with @janeskok.bsky.social, Priscillia developed the "EpiMethylTag" technology to simultaneously assess accessibility with DNA Methylation (2/13)

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Maxim Greenberg @maxvcg.bsky.social · 14/06/2026

Got my Father’s Day gift socks on, which also feel appropriate for my trip to Dublin today for Faseb Biological Methylation #BioMethylSRC 🍻 🍀 🧬

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Maxim Greenberg @maxvcg.bsky.social · 08/06/2026

For students in the Paris region: free mental health support is available, and sessions can be held in Italian, Spanish or English if requested. Don't hesitate to share this info! www.apaso.fr/les-consulta...

Flyer for free mental health support for students. email: etudiants@apaso.fr

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Maxim Greenberg @maxvcg.bsky.social · 04/06/2026

I'm going to start playing this album in the lab to send a subliminal message

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Maxim Greenberg @maxvcg.bsky.social · 01/06/2026

Was completely charmed by my long overdue trip to Strasbourg and @igbmc.bsky.social . Thank you @abardet.bsky.social for being a great host, organizing a walking visit, great dinner, thesis committee, institute visit and talk! Such stimulating discussions, and exciting collaborations to come!!

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Maxim Greenberg @maxvcg.bsky.social · 29/05/2026

Also a good hang @mads100tist.bsky.social @nkonst4.bsky.social . We’ll be on the Seine for a while if you want to join 😉🍻

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Maxim Greenberg @maxvcg.bsky.social · 29/05/2026

The was a straight up awesome talk. Beautiful work, @mads100tist.bsky.social , congratulations!

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Maxim Greenberg @maxvcg.bsky.social · 26/05/2026

I think @upcite.bsky.social is trying to recreate the Maison Fond art installation that used to be in front of Gare du Nord

"Melting House" art instillation at Paris Gare de Nord, raising awareness for climate change

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Maxim Greenberg @maxvcg.bsky.social · 24/05/2026

I went to work in a sweater and a jacket like 3 days ago

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Maxim Greenberg @maxvcg.bsky.social · 23/05/2026

Yes! www.cell.com/cell-reports...

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Maxim Greenberg @maxvcg.bsky.social · 22/05/2026

Maybe this will help sink the stock price!

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Maxim Greenberg @maxvcg.bsky.social · 20/05/2026

Exciting #CtrlEpiEdit conference update: Maja Jagodic @ki.se will be joining us in Paris! Check out her fantastic in vivo epigenome editing preprint here: www.biorxiv.org/content/10.6... And register for the symposium here 😉: ctrlepiedit.sciencesconf.org Please repost!

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Maxim Greenberg @maxvcg.bsky.social · 12/05/2026

Very excited to have Alex Meissner @molgen.mpg.de visit @upcite.bsky.social (jointly hosted by Pierre Defossez from the Epigenetics and Cell Fate Centre and me @ijmonod.bsky.social). If you want to see the cutting edge of DNA methylation in mammals, come to our big amphitheater on June 5!

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Maxim Greenberg @maxvcg.bsky.social · 01/05/2026

This a very important, and extremely well-executed study from Ralph Grand’s group @uniheidelberg.bsky.social. Congrats to all the authors! www.biorxiv.org/content/10.6...

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Maxim Greenberg @maxvcg.bsky.social · 17/04/2026

There are Science papers and then there are *I just dropped and shattered my mug of coffee Usual Suspects style* Science papers www.science.org/doi/10.1126/...

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Maxim Greenberg @maxvcg.bsky.social · 14/04/2026

ElmoFire.gif @mads100tist.bsky.social @nkonst4.bsky.social @ijmonod.bsky.social Looking forward to having Dr. Mad Scientist (AKA Joaquin) grace our humble institute! 🐟🧪

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Maxim Greenberg @maxvcg.bsky.social · 14/04/2026

Someone’s gotta tell the Spelling Bee crew about genomic imprinting disorders

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Maxim Greenberg @maxvcg.bsky.social · 13/04/2026

Trump probably heard the Sinclair Lewis quote “when fascism comes to America it will be wrapped in a flag and carrying across” and said fuck that, just make me Jesus

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Maxim Greenberg @maxvcg.bsky.social · 08/04/2026

I think we could try, but it would mean optimizing the ligation conditions outside of ONT's (validated) workflow. That's why I am reaching out to the community to see if we could avoid the troubleshooting

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Maxim Greenberg @maxvcg.bsky.social · 03/04/2026

Had a cracking lab meeting this morning with our latest epigenome editing results (new tools we're trying for the 1st time). Reminded me we're organizing an awesome symposium in Paris in Nov! Here's our updated flyer (new speaker @dfachinetti.bsky.social). Please share! ctrlepiedit.sciencesconf.org

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Maxim Greenberg @maxvcg.bsky.social · 30/03/2026

When my daughter comes to work

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