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At low dilution (~0.1–1 µg/mL), AbD64138 SpyTag HRP enables detection of otherwise elusive poly-ADPr. At high dilution (~0.01–0.1 µg/mL), it can reduce costs ~10× compared to other tools when poly-ADPr levels are abundant.

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While also available in synthetic rabbit/mouse IgG and biotinylated formats, the community will particularly benefit from the SpyTag HRP format. As with the widely used mono-ADPr antibody AbD43647 (Longarini et al. Mol Cell 2023), the high sensitivity of SpyTag HRP has two important advantages.⬇️

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Obtained by extending our programmable Ser-ADPr technology (Bonfiglio et al. Cell 2020) through a new phage display strategy, this first modular poly-ADPr antibody detects species from di-ADPr to long chains with no cross-reactivity toward mono-ADPr (preprint below; manuscript under revision).

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anti POLY-ADP-RIBOSE Antibody, clone AbD64138 | Bio-Rad

We’ve made the poly-ADPr antibody AbD64138 available in a SpyTag HRP-conjugated format (TZA0117P): www.bio-rad-antibodies.com/monoclonal/h... This sophisticated format enables detection of very low poly-ADPr levels while simplifying and reducing the cost of detecting abundant poly-ADPr.

bio-rad-antibodies.com

Anti poly-ADP-ribose antibody, clone AbD64138, recognizes poly-adenosine diphosphate (ADP)-ribosylation. ADP-ribosylation is a reversible post-translational modification that occurs in multicellular o

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Reposted by The Matić Lab

MPI for Biology of Ageing @mpiage.bsky.social · 10/12/2025

🎉 Celebrating our Amazing Women in Ageing Research! 🎉 Meet Xinping, scientific staff in the proteomics core facility 👩‍🔬 #WomenInScience #AgeingResearch

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The Matić Lab @maticlab.bsky.social · 01/11/2025

Our third PhD student, and third summa cum laude. Congrats, Andreas!

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MPI for Biology of Ageing @mpiage.bsky.social · 24/10/2025

🎉 Celebrating our Amazing Women in Ageing Research! 🎉 Meet Maria, Postdoc in the Matic Lab 👩‍🔬 Maria studies how our cells respond to damage in their DNA — the instruction manual of life. 🧬 @mariapalumbieri.bsky.social

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MPI for Biology of Ageing @mpiage.bsky.social · 07/10/2025

We were delighted to have Nobel laureate Venki Ramakrishnan with us to discuss his exciting new book, "Why We Die." Thanks so much, Venki, for your fascinating and thought-provoking talk - it was truly a pleasure to have you join us!

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Cologne Graduate School of Ageing Research @cga-age.bsky.social · 12/08/2025

Apply now for a fully funded #PhD position at the Cologne Graduate School of Ageing Research and become part of our vibrant #AgingResearch commmuny. 🗓️ Deadline: November 3, 2025. ℹ️ More info: www.ageing-grad-school.de 📣 Spread the news!

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• Together with a series of papers and preprints published this year, it establishes RNF114 as a reader of ADPrUb

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• Observation that serine mono-ADPrUb increases in ARH3 KO cells, raising the intriguing question of its potential role in ARH3 mutation–associated neurodegeneration

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• First reagent specific for ADPrUb, enabling both immunoblotting and immunoprecipitation

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• First mass spectrometric evidence of ADPrUb, regardless of the target residue

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• Serine mono-ADPrUb as a new type of DNA damage-induced histone modification

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• First evidence that, in cells, serine mono-ADPr by the PARP1/HPF1 writer complex is targeted by this unconventional ubiquitylation

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Maria Dilia Palumbieri @mariapalumbieri.bsky.social · 10/07/2025

At its core, it’s a technological paper—necessary to overcome some of the many challenges of working with ADPrUb. But beyond the technical details, below are the main highlights:

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Reposted by The Matić Lab

Thrilled to share my first first-author postdoc publication — a team effort with @aklvnbch.bsky.social. We present the first MS-based evidence of ADP-ribosylation–linked ubiquitin (ADPrUb) in cells after DNA damage. Grateful for all I’ve learned and looking forward to uncovering more about ADPrUb

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Reposted by The Matić Lab

Andreas Kolvenbach @aklvnbch.bsky.social · 09/07/2025

Really happy that our paper is finally released. We show that PARP1 and histones are targets of ADP-ribosyl-linked ubiquitylation during DNA damage and describe how to detect the dual modification. Great teamwork with @mariapalumbieri.bsky.social & thanks to everyone involved @maticlab.bsky.social !

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MPI for Biology of Ageing @mpiage.bsky.social · 27/06/2025

Huge Congratulations to Edoardo José Longarini on receiving the prestigious Peter Hans Hofschneider Prize! 🎉 www.age.mpg.de/424030/Edoar...

age.mpg.de

Edoardo Longarini receives the Peter Hans Hofschneider Prize

Edoardo Longarini receives the prestigious Peter Hans Hofschneider Prize for his exceptional work in molecular medicine.

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Cologne Graduate School of Ageing Research @cga-age.bsky.social · 20/03/2025

🎥We're excited to share the new film about our graduate programme. Get a glimpse of our faculty & PhD students and explore your research opportunities. Join us as we dive into the world of ageing research and discover what makes the CGA truly unique.👩‍🔬🧑‍🎓 www.ageing-grad-school.de/about-us/cal...

ageing-grad-school.de

Discover the CGA film

Get to know our people & programme

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Anthony Leung @leunglab.bsky.social · 13/03/2025

New Paper💡PARP enzymes regulate ADP-ribosylation, a protein modification linked to cancer, infections & neurodegeneration. But it comes with two forms—MARylation (single) & PARylation (multiple). Meet dELTA-MS, a mass spec method that identifies both in one experiment! pubs.acs.org/doi/full/10....

pubs.acs.org

dELTA-MS: A Mass Spectrometry-Based Proteomics Approach for Identifying ADP-Ribosylation Sites and Forms

ADP-ribosylation, characterized by the addition of adenosine diphosphate ribose, can occur in both monomeric (MARylation) and polymeric (PARylation) forms. Little is known about the specific contribut...

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Reposted by The Matić Lab

Mike Cohen @michaelnadbio.bsky.social · 25/02/2025

I'm super excited to share our study in collaboration with @jnpruneda.bsky.social, published today in @embojournal.org! We show that PARPs are modified with a non-canonical ester-linked mono-ADP-ribosylated ubiquitin species for the first time in cells: www.embopress.org/doi/full/10....

embopress.org

Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose | The EMBO Journal

imageimageCertain E3 ligases have been found to ubiquitylate hydroxyl groups on free NAD+ and ADP-ribose in vitro, but the in vivo occurrence of this dual post-translational modification has remained ...

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These new antibody development efforts align with our vision that the fundamental discovery of Serine ADP-ribosylation can be converted into a technology that transforms the study of ADP-ribosylation and related PTMs.

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This preprint is part of a larger manuscript on additional antibodies and their applications to biological questions. The goal of this preprint is to share these poly-ADPr with the community without delay, as we believe they wil benefit many laboratories.

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As with our other tools, the AbD64138 clone should become widely accessible in a few months. For those familiar with our popular AbD43647 antibody, the new AbD64138 clone provides similar advantages for poly-ADPr as AbD43647 does for mono-ADPr.

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We’re particularly excited to share with the community the exceptionally high sensitivity we’ve achieved for poly-ADPr immunoblotting, all while using a faster and simpler protocol enabled by a sophisticated SpyTag-HRP format that eliminates the need for secondary antibodies.

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