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Keith Jolley

@kjolley.bsky.social
632 followers 382 following 93 posts

Bioinformatician in the Department of Biology, University of Oxford. Working on microbial genomics | Developer of @PubMLST.org and BIGSdb | orcid.org/0000-0002-0751-0287

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Reposted by Keith Jolley
uosdɯᴉS ɐuɐꓷ 🦄 @danacorn.bsky.social · 05/09/2026
Doing this a few times has reinforced my opinion that life was terrible until very recently.
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Keith Jolley @kjolley.bsky.social · 28/08/2026
BIGSdb v1.53.5 has been released and adds a new dark mode! This is available for databases on @pubmlst.org. Release notes can be found at github.com/kjolley/BIGS....
Side-by-side views of normal and dark mode for a BIGSdb isolate index page. Normal mode uses light backgrounds with dark text, dark mode uses dark backgrounds with light text.Side-by-side views of normal and dark mode for a BIGSdb isolate query page. Normal mode uses light backgrounds with dark text, dark mode uses dark backgrounds with light text.Side-by-side views of normal and dark mode for a BIGSdb isolate record page. Normal mode uses light backgrounds with dark text, dark mode uses dark backgrounds with light text.
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Keith Jolley @kjolley.bsky.social · 13/07/2026
BIGSdb v1.53.4 has been released. This adds a Sunburst visualisation to the LINvis tool. This uses a series of concentric circles with each ring representing a different LIN threshold with each prefix value at that threshold sized proportionally to its frequency. github.com/kjolley/BIGS...
Sunburst visualisation consisting of concentric circles with segment sizes representing frequency. Some segments have red borders to indicate the location of an isolate following a search
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Reposted by Keith Jolley
Kasia M Parfitt @kasiamparfitt.bsky.social · 12/07/2026
Really happy to share this new pre print on the development of a cgMLST and LIN code for Streptococcus pyogenes, led by Yan Ryan at @ukhsa.bsky.social www.biorxiv.org/content/10.6...
biorxiv.org
High resolution Streptococcus pyogenes core genome MLST and LIN coding scheme for outbreak detection
Streptococcus pyogenes is a globally important pathogen responsible for at least 500,000 deaths a year, causing significant burden on healthcare systems. It is the causative agent for ailments such as...
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Keith Jolley @kjolley.bsky.social · 03/07/2026
BIGSdb v1.53.3 has been released. This brings a visual refresh to the user interface with new fonts, muted colours, and more consistency. Screenshots show side-by-side old and new versions. Release notes at github.com/kjolley/BIGS....
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Reposted by Keith Jolley
Martin Hunt @martibartfast.bsky.social · 26/06/2026
Like Artemis/ACT but wish it was easier to install and more modern? Try Seqhiker. 🧬 Native desktop app (no Java) for Windows/mac/linux 🖱️ Drag-n-drop FASTA/GenBank/GFF3/BAM/VCF files 🔍 Genome browsing and ACT-style comparison ✨ Themes (customisable) and a Vim mode Docs: seqhiker.readthedocs.io
seqhiker.readthedocs.io
The Seqhiker's Guide
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Caro Kobras 🐍 @carokobras.bsky.social · 14/06/2026
New paper 🧪🧬🧫GeneScanner is our new tool to profile genetic variation in bacterial populations. Use via GitHub or @pubmlst.org. Thanks to Ko and Pri @sheppardlab.bsky.social @broncio.bsky.social @kjolley.bsky.social @microbiologysociety.org @imibirmingham.bsky.social @ineosoxford.bsky.social
microbiologyresearch.org
GeneScanner: profiling genetic variation across bacterial populations
Rapid, low-cost genome sequencing has transformed microbiology, advancing efforts to link genetic and phenotypic variation across diverse bacterial systems. Laboratory functional screens now uncover c...
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Reposted by Keith Jolley
sylvain Brisse @sylvainbrisse.bsky.social · 04/06/2026
It's out! The LIN code approach for genomic taxonomy of microbial strains and its applications in genomic epidemiology journals.plos.org/plosbiology/...
journals.plos.org
Life Identification Numbers: A strain nomenclature approach to aid epidemiological surveillance of bacterial pathogens
Unified bacterial strain taxonomies are needed for coherent communication of findings in microbiological research. This Essay provides an overview of a novel bacterial strain taxonomy and describes ho...
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Reposted by Keith Jolley
Led By Donkeys @ledbydonkeys.org · 17/05/2026
Immigration make Britain brilliant
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Keith Jolley @kjolley.bsky.social · 14/05/2026
BIGSdb v1.53.2 has been released and is now live on @pubmlst.org. This adds functionality to the LINvis plugin. This includes annotation of nodes representing specific LIN prefixes entered as a list.
LINvis with a popup box that allows a user to enter a list of LIN code prefixes for annotation.
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Keith Jolley @kjolley.bsky.social · 07/05/2026
BIGSdb v1.53.1 has been released. This version adds additional password complexity checks and an option to automatically register new accounts for all available databases. This is now live on @pubmlst.org. Release notes at github.com/kjolley/BIGS....
github.com
Release Version 1.53.1 · kjolley/BIGSdb
What's new? Added additional password complexity checks. Added option to automatically register newly validated site-user accounts for all available databases. Fix for determining if FASTA file is...
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Keith Jolley @kjolley.bsky.social · 01/05/2026
BIGSdb v1.53.0 has been released. This version addresses an issue that has been raised by PubMLST users seeking easier authenticated access to the @pubmlst.org API. We have now introduced personal API keys that can be used instead of OAuth authentication for ad-hoc scripts and pipelines.
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Caro Kobras 🐍 @carokobras.bsky.social · 23/04/2026
Super exciting news 🚨 I am hiring a technician to join my brand new lab at the University of Birmingham! @imibirmingham.bsky.social Come and support my BBSRC-funded research on understanding how staphylococci survive antibiotics. Please share! 🧫🧪 www.jobs.ac.uk/job/DRH271/a...
jobs.ac.uk
Assistant Technical Specialist at University of Birmingham
Searching for an academic job? Explore this Assistant Technical Specialist opening on jobs.ac.uk! Click to view more details and browse other academic jobs.
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Sheppard Lab @sheppardlab.bsky.social · 15/04/2026
PhD student Priyanshu Raikwar showcasing his research on origin and evolution of life at #Microbio26 ; Microbiology society annual conference 2026 ! Do find him to know about the application of genomics, proteomics, ML in evolutionary biology. @microbiologysociety.org @ineosoxford.bsky.social
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Kasia M Parfitt @kasiamparfitt.bsky.social · 15/04/2026
If you want to hear about LIN codes and their application to meningococcal outbreaks, then please come along to my talk at 4:45pm today in the Transformative Tools session at @microbiologysociety.org #MicroBio26 #omics
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Kasia M Parfitt @kasiamparfitt.bsky.social · 24/03/2026
Really pleased to see our work (LIN codes developed for high-resolution typing of meningococci) has been directly utilised in the @ukhsa.bsky.social meningococcal disease outbreak technical briefing
gov.uk
Invasive Meningococcal Disease outbreak 2026: technical briefing 1
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Kasia M Parfitt @kasiamparfitt.bsky.social · 04/03/2026
I am excited to share a pre print for one of the main outputs from my current postdoc, that I have been working on for a couple of years. This LIN code system for N. meningitidis adds a layer of stability for high resolution typing of the meningococcal population.
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Keith Jolley @kjolley.bsky.social · 02/03/2026
BIGSdb v1.52.1 has been released. This adds 2 new plugins for LIN code visualisation - LINtree (@giphy-ip.bsky.social) and LINvis. See github.com/kjolley/BIGS... for release notes. BIGSdb powers all the databases at @pubmlst.org and BIGSdb Pasteur.
Tree drawn within the iTOL interface generated by LINtree. The tree is annotated with LIN codes and clonal complexes in different colours.LINvis displays datasets of LIN codes as a series of hierarchical packed circles.
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Reposted by Keith Jolley
Ben Pascoe @benizao.bsky.social · 14/02/2026
In Iquitos, Peru this week 🇵🇪 working on enteric pathogen genomics and surveillance in the Amazon. Science powered by collaboration, rain, food… and Inca kola!
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Reposted by Keith Jolley
Tabea Elsener @tabeaelsener.bsky.social · 13/01/2026
Neisseria gonorrhoeae carries an unusually large number of restriction–modification systems (up to 16!), many of which are phase-variable. How does this affect plasmid transmission in gonococci? doi.org/10.64898/202...
doi.org
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sylvain Brisse @sylvainbrisse.bsky.social · 30/11/2025
Huge preprint if you are interested in bacterial strain taxonomy! The why and how of cgMLST LIN codes: An extensively revised and expanded version doi.org/10.1101/2024... I will summarize it for you in this thread 👇
doi.org
Life Identification Numbers: A bacterial strain nomenclature approach
Unified strain taxonomies are needed for the epidemiological surveillance of bacterial pathogens and international communication in microbiological research. Core genome multilocus sequence typing (cg...
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Keith Jolley @kjolley.bsky.social · 25/11/2025
BIGSdb v1.15.6 has been released. This contains 2 new plugins - GeneScanner and a wrapper for PlasmidFinder. Also added support for third party analysis fields for various plugins and performance improvements. Release notes at github.com/kjolley/BIGS...
github.com
Release Version 1.51.6 · kjolley/BIGSdb
This release adds two new analysis plugins and other improvements: New GeneScanner plugin New PlasmidFinder plugin Updates to various plugins to support analysis fields (third-party results stored...
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Reposted by Keith Jolley
sylvain Brisse @sylvainbrisse.bsky.social · 24/10/2025
The recordings of the 6-hour webinar on Bacterial Strain Taxonomy for Genomic Surveillance are now available through the @ecdc.europa.eu ECDC Learning portal: learning.ecdc.europa.eu/course/view.... - cgMLST, SNPs, K-mer based strain nomenclatures applications and more!
learning.ecdc.europa.eu
Course: GenEpi-BioTrain - Virtual training 21 - Bacterial Strain Taxonomy for Genomic Surveillance
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sylvain Brisse @sylvainbrisse.bsky.social · 21/10/2025
Almost there! Starting tomorrow morning (Paris time), the webinar on Bacterial strain Taxonomy for Genomic Surveillance organized within the ECDC sponsored GenEpi-BioTrain programme; registration is free; see more here: research.pasteur.fr/en/event/bac...
research.pasteur.fr
Bacterial strain Taxonomy for Genomic Surveillance - GenEpi-BioTrain Webinar - Research
This workshop aims to provide participants with a comprehensive understanding of bacterial classification and nomenclature approaches used in genomic surveillance. Participants will gain insight into ...
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Keith Jolley @kjolley.bsky.social · 17/10/2025
New cgMLST scheme and LIN code classifications for Moraxella catarrhalis. Available on @pubmlst.org. www.ndph.ox.ac.uk/news/new-stu...
ndph.ox.ac.uk
New study confirms genetic lineages of common bacterium
Oxford Population Health study confirms two genetic lineages of M. catarrhalis and unveils a global tool to track strains and resistance.
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Reposted by Keith Jolley
CampyUK2025 @campyuk2025.bsky.social · 25/09/2025
Ben Pascoe @benizao.bsky.social @sheppardlab.bsky.social shining a light on C. coli - although less common than C. jejuni, it has higher levels of AMR. His work in Peru highlights the importance of this pathogen in LMICs due constant exposure with backyard livestock and antibiotic use 🇵🇪 #CampyUK2025
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Gc Rox @gcrox.bsky.social · 06/09/2025
Postdoc position (please re-post) A funded postdoctoral position, will open in 2026 to study aspects of Neisseria gonorrhoeae pathogenesis, antigenic variation, genetics, or physiology. If interested in more information, please contact Hank Seifert h-seifert@northwestern.edu #MicroSky
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Reposted by Keith Jolley
PubMLST.org @pubmlst.org · 27/08/2025
A new MLST scheme is now available for Actinobacillus spp. at pubmlst.org/organisms/ac.... The scheme was developed by Nick Vereecke and colleagues at Ghent University and PathoSense, Belgium. It is described in pubmed.ncbi.nlm.nih.gov/37020296/.
pubmlst.org
Actinobacillus spp.
The MLST scheme hosted in this database was developed by Nick Vereecke and colleagues at Ghent University and PathoSense, Belgium. It is described in Vereecke et al. 2023 Vet Res 54:33. Database curat...
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Reposted by Keith Jolley
Kelly Wyres @kelwyres.bsky.social · 22/07/2025
Woohoo Kaptive is now integrated within BIGSdb for #Klebsiella and #Acinetobacter. Thanks @kjolley.bsky.social! Also like to acknowledge that the Acineto dbs were developed and maintained by Johanna Kenyon and team at Griffith Uni.
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Keith Jolley @kjolley.bsky.social · 18/07/2025
BIGSdb v1.51.4 has been released. This adds a new #Kaptive plugin for surface polysaccharide typing of Acinetobacter baumannii and Klebsiella. github.com/kjolley/BIGS... for details. Kaptive is developed by @tomstantonmicro.bsky.social, @kelwyres.bsky.social , @katholt.bsky.social and colleagues.
Output from Kaptive analysis shown in a BIGSdb isolate record. Tabular results are shown followed by a graphical representation of the genes within the locus analysed.
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Keith Jolley @kjolley.bsky.social · 14/07/2025
BIGSdb v1.51.3 has been released. This provides performance improvements to distance matrix calculation in Genome Comparator, by rewriting the pairwise comparison in C. In addition, iTOL and Microreact trees can now be constructed from allelic profiles, not just sequences. github.com/kjolley/BIGS...
github.com
Release Version 1.51.3 · kjolley/BIGSdb
This version provides the following enhancements: Distance matrix calculation is now significantly quicker in Genome Comparator. Various optimisations were made to avoid redundant hash lookups and...
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sylvain Brisse @sylvainbrisse.bsky.social · 25/06/2025
@kjolley.bsky.social presenting challenges of maintaining genomic epidemiology platforms for public health surveillance of pathogens @pasteur.fr #BIGSdb
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sylvain Brisse @sylvainbrisse.bsky.social · 12/06/2025
The BIGSdb-Pasteur project, running under my supervision since 20 years, is looking for a project manager - great opportunity to contribute to this high-profile international resource in the field of reference strain taxonomies, genomic epidemiology and antimicrobial resistance surveillance
research.pasteur.fr
Project manager: Genomic taxonomy of bacterial strains, epidemiological surveillance and antimicrobial resistance - Research
We are looking for a project manager to develop our activities around the genomic taxonomy of bacterial strains and the corresponding platform at https://bigsdb.pasteur.fr/. The project manager’s main...
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KlebNET-GSP @klebnet.bsky.social · 21/05/2025
We are pleased to launch the KlebNET Genomic Epidemiology Consortium! We aim to build a public metadata repository; systematic risk framework for global genomic surveillance; and genomic epi reviews for high-impact #Klebsiella clones. Join us here: klebnet.org/klebnet-gsp-... #ABPHM25
klebnet.org
KlebNET-GSP
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Keith Jolley @kjolley.bsky.social · 07/05/2025
New preprint describing a cgMLST scheme and LIN code system for Moraxella catarrhalis, with database hosted on @pubmlst.org. www.biorxiv.org/content/10.1...
biorxiv.org
Understanding the population structure of Moraxella catarrhalis using core genome multilocus sequence typing (cgMLST) and a life identification number (LIN) code classification system
Moraxella catarrhalis is an important cause of infectious exacerbations of chronic obstructive pulmonary disease and otitis media. Previously, seroresistant (SR) and serosensitive (SS) lineages that d...
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Tabea Elsener @tabeaelsener.bsky.social · 07/05/2025
Very happy to see the second paper of my PhD now published in PLOS Pathogens! dx.plos.org/10.1371/jour...
dx.plos.org
Origin, evolution, and success of pbla, the gonococcal beta-lactamase plasmid, and implications for public health
Author summary Neisseria gonorrhoeae is a threat to sexual and reproductive health. With no available vaccine against gonococcal disease, control of infection depends on having effective antimicrobial...
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Willem van Schaik @wvschaik.bsky.social · 02/05/2025
One of the many problems of the insane First Past The Post system this country uses for its elections, is that it completely breaks down when four or more parties are in with a shout, and the voter needs to decide what party to vote for to keep the swivel-eyed loons out.
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Lilly Cummins @lillycummins.bsky.social · 25/04/2025
Very pleased to have been awarded this grant to collaborate with researchers at the University of Oslo!
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Caro Kobras 🐍 @carokobras.bsky.social · 17/04/2025
Super excited to have been awarded a 2024 BBSRC Fellowship to investigate antibiotic tolerance in staphylococci! ☺️🧫🦠💊 @ukri.org Very grateful for all the support I have received!! www.ukri.org/news/9-milli...
ukri.org
£9 million investment fuels groundbreaking bioscience research
From unlocking the secrets of animal movement to tackling antimicrobial resistance, UK’s brightest bioscience minds are pushing the boundaries of innovation.
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Sheppard Lab @sheppardlab.bsky.social · 07/04/2025
TWO postdoc positions in Computational Microbiology and/or biochemistry. Join our interdisciplinary group in the new Life and Mind Building @OxfordBiology, @UniofOxford, @IneosOxford Please apply and share this post: www.jobs.ac.uk/job/DMP641/p...
jobs.ac.uk
Postdoctoral Research Associate at University of Oxford
Discover Postdoctoral Research Associate jobs and more in higher education on jobs.ac.uk. Apply for further details on the top job board.
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CampyUK2025 @campyuk2025.bsky.social · 22/02/2025
🚨 We're very happy to announce the #CampyUK2025 meeting in Oxford (@ox.ac.uk), which will focus on #AMR in collaboration with @ineosoxford.bsky.social 🗓️Save the date: 24th-25th September 2025 📝Register your interest on our website: campyuk.web.ox.ac.uk
campyuk.web.ox.ac.uk
Home
Why now? Campylobacter infections are on the rise globally, with increasing levels of antimicrobial resistance (AMR) making treatment more challenging. As the world’s most common bacterial cause of gastroenteritis, Campylobacter poses a growing public health threat, yet effective interventions remain limited. There is an urgent need for research into novel and sustainable strategies to mitigate its impact—spanning genomics, surveillance, food safety, and public health interventions.
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PubMLST.org @pubmlst.org · 10/02/2025
A new database is now available on @PubMLST.org for Halobacteria MLST. This is our first scheme for Archaea. The scheme is based on MLSA loci described in Papke et al. 2011 (pubmed.ncbi.nlm.nih.gov/21296924/). Database is curated by Kayo Cesar Bianco Fernandes (FIOCRUZ). pubmlst.org/organisms/ha...
pubmed.ncbi.nlm.nih.gov
A multilocus sequence analysis approach to the phylogeny and taxonomy of the Halobacteriales - PubMed
Members of the order Halobacteriales are obligate extreme halophiles that belong to the domain Archaea. The classification of the Halobacteriales currently relies on a polyphasic approach, which integ...
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James Zlosnik @jameszlosnik.bsky.social · 11/01/2025
www.nature.com/articles/d41...
nature.com
These are the 20 most-studied bacteria — the majority have been ignored
Model microbes such as Escherichia coli hog scientists’ attention, leaving most known bacteria with few publications devoted to them.
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Tabea Elsener @tabeaelsener.bsky.social · 08/01/2025
A hitchhiker’s guide to the gonococcal population: biorxiv.org/content/10.1...
biorxiv.org
The origin and evolution of the gonococcal beta-lactamase plasmid, and implications for public health
Neisseria gonorrhoeae is a leading cause of sexually transmitted infection (STI) and a priority AMR pathogen. Two narrow host range plasmids, p bla and pConj, have contributed to ending penicillin and...
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Keith Jolley @kjolley.bsky.social · 08/01/2025
BIGSdb v1.50.1 has been released. This adds support for Neighbour-Joining trees in GrapeTree. Full release notes at github.com/kjolley/BIGS...
Neighbour-joining phylogenetic tree with coloured nodes at the ends of branches representing clonal complexes.
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Alan McNally @alanmcn1.bsky.social · 01/01/2025
Being treated to the most spectacular show to start the year
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rael gost pictuers @jackfeerick.bsky.social · 01/01/2025
One of my favorites of the genre (though relatively late in the game) is Val Bennett's "The Russians Are Coming," (1968) which takes the Dave Brubeck Quartet's "Take Five" and says, "What would this sound like in 4-4 time *and* with a ska beat?"
youtube.com
VAL BENNETT - The Russians Are Coming [1968]
YouTube video by Bionic Dub
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Keith Jolley @kjolley.bsky.social · 19/12/2024
Just published - A new cgMLST scheme for Borrelia burgdorferi, the causative agent of Lyme disease. www.cell.com/cell-reports.... Scheme is available on @pubmlst.org.
cell.com
A core genome MLST scheme for Borrelia burgdorferi sensu lato improves insights into the evolutionary history of the species complex
Hepner et al. develop a 639-loci core genome multi-locus sequence typing (cgMLST) scheme for Borrelia burgdorferi sensu lato that extends the traditional 8-loci MLST to a genomic scale. The cgMLST scheme gains higher reliability, robustness, and resolution, enabling improved insights into the evolutionary history of the species complex.
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Nick Tsergas @nicktsergas.ca · 28/11/2024
You guys remember TweetDeck? Bluesky has it, and it's free. Check out 🔗 deck.blue by @gildaswise.com for a multi-column interface. 2/10
Screenshot of the deck.blue UI
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Willem van Schaik @wvschaik.bsky.social · 02/12/2024
Deadline for applications is midnight (GMT) today!
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