New preprint from the lab! Riboswitches toggle by rearranging base pairs. Can you get the same toggling without changing the secondary structure? Turns out yes. We designed a minimal modular RNA that forms or breaks a tertiary contact in response to ligand binding.
www.biorxiv.org/content/10.6...
biorxiv.org
Single-base substitutions switch RNA tertiary allostery between positive and negative coupling.
Riboswitches regulate gene expression in response to ligand binding, remodeling their secondary structure to terminate transcription or block translation. This innate switching has driven efforts to r...
