Reposted by Hao Zhu Lab at UT Southwestern
In August's #EditorsChoice, Gianna Maggiore, @haozhulab.bsky.social & co showed strong clinical potential in reducing #HepatocellularCarcinoma by targeting the actin-binding protein anillin via #siRNA knockdown in #MouseModel.
⚡ doi.org/10.1242/dmm....
📰 doi.org/10.1242/dmm....
doi.org
Chemoprevention of hepatocellular carcinoma using N-acetylgalactosamine-conjugated siRNAs
Editor's choice: Comparison between five N-acetylgalactosamine-conjugated siRNAs in several mouse models of liver cancer found ANLN to be a promising target for hepatocellular carcinoma…
![siCdk1 and siAnln decreased tumor burden in the DEN/PB HCC model. (A) Schematic overview of DEN/PB mouse model. At 2 weeks of age, male mice on the C3H strain background were given a single injection of DEN (25 mg/kg) 4 weeks before the start timepoint (-4w). At 6 weeks of age, mice were placed on 0.05% PB drinking water [start timepoint of 0 weeks (0w)]. Mice were injected with siRNA every other week starting at 12 weeks of age (6w), for a total of nine doses (with 5 mg/kg per dose) and sacrificed 1 week after the final dose (23w). (B) Liver weights for DEN/PB mice at 23 weeks after starting PB water, i.e. the final time point. (C) Liver-to-body-weight ratio for DEN/PB mice at the final time point. (D) Representative images of livers from DEN/PB mice at the final time point. (E) Surface tumor numbers were quantified using front and back images of each liver. (F) Representative immunofluorescence images of DEN/PB livers obtained from mice as indicated. Staining was against GS (red) to indicate the degree of WNT activation. Tumors are outlined by yellow dashed lines. One GS+ tumor was seen in the PBS group. Nuclei were stained with DAPI (blue). Scale bars: 800 μm. For panels B, C and E, the P-values with respect to the PBS group are shown above each group.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:5jupfg23qpjk2643o6aeftly/bafkreia346vromeksdvvhk4ikwhmd5jzwh35ijdqviiprwfwklidcshhqm)
