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Scientific Reports - Low-resolution FAIMS for increased peptide coverage in low-load and single-cell proteomics

Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00430

High-throughput proteomics is critical for understanding biological processes, enabling large-scale studies such as biomarker discovery and systems bi…

Technical lead Johannes Petzoldt tells the story behind the development of Thermo’s Orbitrap Astral Zoom Mass Spectrometer

The Proteograph Product Suite, a multiplexed nanoparticle (NP) protein corona-based workflow, substantially improves the depth of detection of proteins by mass spectrometry (MS) by compressing the dyn...

Field Asymmetric Ion Mobility Spectrometry (FAIMS) enhances signal to noise ratio filtering ions based on their differential mobility, making it an indispensable tool for single-cell and low-input pro...

High-throughput proteomics is critical for understanding biological processes, enabling large-scale studies such as biomarker discovery and systems biology. However, current mass spectrometry technolo...

High-throughput mass spectrometry-based proteomics has gained increasing interest for both academic and industrial applications. As implementation of faster gradients has facilitated higher sample thr...

Meet one of the greatest innovators in the history of mass spectrometry, hard at work.

Cellular differentiation processes are largely orchestrated by variation in transcription factor (TF) abundance. Since these proteins are usually expressed at extremely low levels, studying TF-driven ...

Liquid chromatography-mass spectrometry (LC-MS) can enable precise and accurate quantification of analytes at high-sensitivity, but the rate at which samples can be analyzed remains limiting. Throughp...

Rationale Hybrid mass spectrometers combine multiple mass analyzers to achieve optimal performance in terms of tandem mass spectrometry, high mass resolving power, and mass measurement accuracy for ...

Chip-Tip is a label-free quantification-based single-cell proteomics workflow for deep single-cell proteomics, which identifies over 5,000 proteins and 40,000 peptides in single HeLa cells.

In BriefWe present deep analysis of the human proteome in less than 1 h. The Orbitrap Astral mass spectrometer, alongside advances in sample preparation, method development, and data processing, is ushering in a new era of proteomics where a harmony of throughput, depth, and sensitivity are enable studies of unprecedented impact. We contextualize these results within the landscape of human proteomics. Comparisons with previous instrumentation in interrogating gene knockouts demonstrate the potential of proteomics to elevate biological investigation in years to come.

The advancement of crosslinking mass spectrometry (CLMS) has significantly enhanced the ability to study protein-protein interactions and complex biological systems. This study evaluates the performan...