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GlycoShape @glycoshape.org · 25/09/2026

Refreshed ReGlyco Filter in bioRxiv 🧪(doi.org/10.64898/202...) in view of the upcoming Protein Design Competition on Monday 28/09 🎉🔥 proteinbase.com/competitions... Each week a new target and if it's a glycoprotein, glycoshape.org and ReGlyco Filter can help screen and optimise your binder design

NiV G was the target of the Adaptyv Bio Protein Design Competition 2025. Structure of the Nipah Virus Glycoprotein (NiV-G) homotetramer (aa 92-602) reconstructed from cryo-EM structures (PDB 7TXZ and 7TY0) bound to broadly neutralising antibody nAH1.3 Fabs(Wang et al. 2022) (not shown). Static (single) glycans 3D structures (shown with sticks in blue) were reconstructed with GlycoShape ReGlyco(Ives et al. 2024). The glycoform selection was guided by glycoproteomics analysis(Hawkins et al. 2025). The visible six glycan sites on chains A, B and D are mapped onto the structure, while glycans on chain C are not labelled for clarity

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Elisa Fadda @elisafadda.bsky.social · 16/09/2026

Fantastic opportunity to talk, listen and discuss the future of #glycotime 🧪 with ECRs and PhD students at the upcoming GRS Glycobiology 2027 organised by the über talented @j-a-n-alexander.bsky.social and @lornamilne.bsky.social who put together a sensational programme. Register asap!! ⬇️

GRS flyer with a view of the town of Ventura (CA) from the hills, tree on the right hand side, sea in the distance. Flyer has a blue background

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Elisa Fadda @elisafadda.bsky.social · 14/09/2026

🚨 Want to be part of top #glycotime on the seaside in the California sunshine? Register to the 2027 GRC Glycobiology ⬇️ Stacy and Lance put togteher up one of the most exciting programmes I have ever seen with phenomenal keynotes! We have 32 abstracts to select, so the next speaker could be YOU! 😎

Announcement of the GRC Glycobiology 2027 to be held in Ventura CA on March 14 to 19, 2027. Blue background with an photo of Ventura from the visitventuraca.com

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GlycoShape @glycoshape.org · 04/09/2026

🚨 New version of GlycoShape is now live at glycoshape.org 🥳 Powered by crabWURCS everything runs on your browser and while you save all you ReGlyco work and go back to it whenever you like 😎 Among other new things: new slick drawer, much easier to use, new slick SNFG representations and new layout

screenshot to represent the new layout of GlycoShape, here the database screen added NEW sticker

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Elisa Fadda @elisafadda.bsky.social · 02/09/2026

I will be in Geneva at #ECCB2026 for a super #glycotime workshop in glyco-bioinformatics hosted by @glycoexpasy.bsky.social to present a hands-on guide to the new layout and features of @glycoshape.org If you want to hear all about it, just sign up! ⬇️ eccb2026.org/communities-... online and free

screenshot of the title slide of my talk showing a graphical representation of a cell surface in brown with glycans added dfrom structures in Glycoshape. the title of the talk reads 3D glyco-bioinformatics with GlycoShape

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Nick Riley @nmriley.bsky.social · 28/08/2026

We then took a deeper dive into our EpCAM data to look at what integrating bottom-up glycopeptide and intact glycoproteoform measurements can get us, and @emmajays.bsky.social helped us use @glycoshape.org to model how a glycoproteoform with two occupied N-glycosites might look like.

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GlycoShape @glycoshape.org · 28/08/2026

Want to know more about #glycotime resources in bioinformatics and/or build some glycoproteins with GlycoShape? Join us online on Sept 4th for fabulous hands-ON workshops organised by @glycoexpasy.bsky.social 😎🥳🧪 Online registration is FREE (Price: 0 CHF) here, eccb2026.org/communities-...

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GlycoShape @glycoshape.org · 17/07/2026

The fully glucosylated LLO structure is the preferred substrate of eukaryotic OST, especially engineered to enhance N-glycosylation efficiency; but how does that work? and why do we need all those glucoses? @beatricetropea.bsky.social answers all these Q in a new preprint ⬇️ doi.org/10.64898/202...

doi.org

How the terminal glucoside of the N-glycan donor affects the catalytic efficiency of the eukaryotic oligosaccharyltransferase

The eukaryotic oligosaccharyltransferase (OST) is the enzyme responsible for initiating N-glycosylation of secreted proteins by transferring a pre-assembled lipid-linked oligosaccharide (LLO) donor to...

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Elisa Fadda @elisafadda.bsky.social · 02/07/2026

As a fun educational/inspirational #glycotime we decided to curate a Blog on glycoshape.org, you can find it under Resources or here glycoshape.org/blog You can *sign up* to get an email when we post a new entry The latest is E04:Let's talk about glycan binding to viral (glyco)proteins 🧪

Figure 1: Left. Structure of the SARS-CoV-2 S (gamma strain ectodomain; PDB 7sbs). Chains are represented in different colours, pink for chain A, grey for chain B and green for chain C, also in the legend at the bottom left corner. Right. Structure of an isolated RBD (grey surface) from the MD trajectory in complex with a GM1 oligosaccharide (C atoms in purple, O in red). The N345 glycan is not shown for clarity. The structures of the GM1 and GM2 oligosaccharides are shown below the RBD structure with SNFG symbols.

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GlycoShape @glycoshape.org · 30/06/2026

Un-crystallisable glyco-complexes anyone? New work led by John Klassen's lab introduces antibody masking-native mass spectrometry (AM-nMS) a spatially resolved assay that allowed us to map glycan binding sites across the SARS-CoV-2 RBD. The results match our MDs! 😜 All details glycoshape.org/blog

screenshot of the E04 new blog entry on https://glycoshape.org/blog titled Let's talk about glycan binding to viral (glyco)proteins

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GlycoShape @glycoshape.org · 19/06/2026

glycoshape.org has now a blog where we discuss (yep you got it) glycans, glycoproteins and fun stuff! Season 1 is out with: Ep01: Why should we care about glycan heterogeneity? Ep02: How can we include glycan heterogeneity in a 3D framework? Ep03: Molecular precision glycoscience: Enter Siglecs

Header of the Blog page on https://glycoshape.org/blog that you can find in the drop-down menu under Resources> Blog

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GlycoShape @glycoshape.org · 09/06/2026

Next time you hear 'glycans are just a fancy protein decoration' 😱 show them this fabulous work led by Katrine Schjoldager and team at the CGR 🇩🇰 with us 🇬🇧 showing how The ligand preference of LRP1 is regulated by O-glycans Out now www.science.org/doi/10.1126/... 🧵1/10 ⬇️

science.org

The ligand preference of LRP1 is regulated by O-glycans

GALNT11-mediated O-glycans modulate LRP1 uptake of tau and Aβ, unveiling glycan-driven mechanisms in neurodegenerative pathways.

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GlycoShape @glycoshape.org · 05/06/2026

Glycoheterogeneity is an aspects of glycobiology that most would class into a category between difficult and intolerable, but @benschulz.bsky.social and us love it! So we wrote a whole review in Current Opinion in Structural Biology on it 🧵1/8 ⬇️ doi.org/10.1016/j.sb...

screenshot of the the title of the review "Heterogeneity of glycoproteins: Why does it matter and how to account for it"

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Elisa Fadda @elisafadda.bsky.social · 03/06/2026

🚨 #glycotime seminar alert! June 22nd, 2026 4pm JST (8am BST) @kiyokof.bsky.social will present the GlyTouCan v.4 in the next GlySpace Alliance Seminar event 🔥 If you use @glycoshape.org, you know already aboutGlyTouCan IDs. Tune in ⬇️ to discover what GlyTouCan can do for your research 🥳

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GlycoShape @glycoshape.org · 02/06/2026

Carbohydrate-binding is a small profit affair, so proteins often adopt complex multidomain architectures enabling a mechanism known as 'binding cooperativity', where binding to one monomer contributes to the binding affinity of the whole systems BUT how does it actually work? 🔥 #glycotime 🧵1/4 ⬇️

static.klipy.com

Alexis Rose: I Don't Know You Tell Me (Schitt's Creek)

ALT: Alexis Rose: I Don't Know You Tell Me (Schitt's Creek)

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Elisa Fadda @elisafadda.bsky.social · 07/05/2026

A fab, sunshiny week in 🇩🇰! Great #glycotime 🧪 at the CBM16 in Helsingør, with posters Bea: regulation of N-glycosylation by OST (doi.org/10.1101/2025...) Silvia: Siglec-6 high-recision recognition of glycolipids (doi.org/10.1038/s420...) Ojas: de novo binders design (doi.org/10.64898/202...) 🧵⬇️

From the left, Elisa, Ojas, Bea and Silvia. Blue skies above Helsingor, Denmark for the CMB16

Silvia (left) introducing her work on Siglec 6 (https://doi.org/10.1038/s42003-026-09609-8) in a flash talk at CBM16

Elisa (small with red jumper in the left corner) presenting GlycoShape in her "work along" part of the talk, with people in the audience rebuilding the glycosylation of IL5 on GlycoShape ReGlyco

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GlycoShape @glycoshape.org · 24/04/2026

Daily job count is quite high these days after the release of our case study 1 week ago and of the ReGlyco de novo Binder Design Filter notebook ⬇️ doi.org/10.64898/202... and res. therein So exciting to see a growing number of researcher including glycosylation in their studies! 🎉 #glycotime 🧪

Bar graph showing the GlycoShape job count x day in April 2026

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GlycoShape @glycoshape.org · 21/04/2026

We are proud to be part of @elixiruknode.org as the @unisouthampton.bsky.social node, and to share our expertise in structural and computational glycoscience 🧪, together with our OA resources thought it. Check out the announcement below for more information ⬇️ 🥳

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GlycoShape @glycoshape.org · 17/04/2026

Most biologics are glycosylated and some of them heavily. In this “fresh off the press" #glycotime preprint we look into how a filter that accounts for glycosylation explicitly in 3D can help reduce lab costs and increase the efficiency of de novo binder design pipelines. Short 🧵 ⬇️ 1/7

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GlycoShape @glycoshape.org · 30/03/2026

Super congratulations to the Siglec (and many other stories) extraordinaire Dr. D'Andrea!!! 👩‍🎓🎉🥂🍾 A huge thank you to the fantastic examiners Alba Silipo (external) and Trinidad Velasco-Torrijos (internal) for your expert and in-depth examination, which made Silvia's viva a great day to remember 😎

From left: Prof Alba Silipo (UniNa, Federico II), Elisa Fadda, Dr Silvia D'Andrea, and Dr Trinidad Velasco-Torrijos, yellow table and amazing red velvet cake

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GlycoShape @glycoshape.org · 19/03/2026

Super congratulation to Dr Akash Satheesan from the glycoShape team, who graduated officially today with a PhD @maynoothuniversity.ie !! 😎🎓👏🥳🥂 The 🔺-red gown definitely suits you well Akash! 😍

PhD graduation photo of Dr Akash Satheesan. From the left Beatrice Tropea, Akash (showing his well deserved parchment), Ojas Singh and Silvia D'Andrea

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GlycoShape @glycoshape.org · 02/03/2026

Lectins are known to be low affinity binders with relatively broad glycan target preference. In this work live in @commsbio.nature.com we show how Siglecs change this paradigm, acting as molecular precision tools required to fine tune immune response 🤯 #glycotime 🧵⬇️ doi.org/10.1038/s420...

doi.org

Glycolipid recognition and binding by Siglec-6 hinges on interactions with the cell membrane - Communications Biology

Siglecs are immunoregulatory lectins with very similar architectures. The authors show how the V-set domain of Siglec-6 allows it to select for precise sialylation patterns in specific biological envi...

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GlycoShape @glycoshape.org · 03/01/2026

Happy new year 2026 #glycotime! 🥳 We just introduced some useful options to Re-Glyco Ensemble. In the Advanced Settings you can now select a seed (positive integer) to reproduce structural ensemble of your favourite glycoforms such as the one below that you will see soon in an upcoming paper,

Structure of a protein (cyan/teal) rendered as surface with 50 frames representing the dynamic ensemble of the glycans (white surface and sticks) depicted at the three sites (N93, N99 and N104) indicated by the 2D SNFG symbols and corresponding GlyTouCan IDs. Graphic rendering with VMD

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GlycoShape @glycoshape.org · 15/12/2025

Happy birthday to us! 🥂🎂🥳 We are only 2 yo, but so much #glycotime happened since Dec 2023! ⬇️🧵 We truly appreciate your support, you fabulous glycoengineers around the world and we have so many exciting new tools for you coming up in 2026 to make your 3D glycoforms even better and faster! 🤩

Christmas wreath obtained by photoshop (gimp.org) of a protein structure with glycans in white rebuilt with Re-Glyco, protein in orange surface, red beads and green cartoons from PDB 8DLO

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Carolyn Bertozzi @carolynbertozzi.bskyverified.social · 11/12/2025

I love this thread by @glycoshape.org, this is @bsky.app at its best!

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GlycoShape @glycoshape.org · 11/12/2025

Super interesting work from @carolynbertozzi.bskyverified.social group about the effects of glycosylation in protein structure and function at the proteome scale 🤯💥🤩 Results show that sialylation and fucosylation are crucial, which makes a lot of sense. We did some extra bits 🧐 with GlycoShape ⬇️🧵

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GlycoShape @glycoshape.org · 05/12/2025

We just did some maintenance and updates to glycoshape.org, ✅ Fancy background with floating SNFG symbols 🤩 ✅ 'Latest Updates' feed from this account on bsky 🤩 ✅ Download PDB with SASA values in Re-Glyco Ensemble 🤩 Check it out!

Front page of Glycoshape website with the new background with floating SNFG symbols representing different monosaccharides. In the website the background is animated, so check it out at https://glycoshape.org

When you rebuild a glycosprotein with Re-Glyco Ensemble, now you can download the multiframe PDB with SASA values in the B-values column. The structure on the left shows in blue all the accessible surface and in light blue and red inaccessible residues due to the glycan presence. The buttons below are in cyan for regular multiframe PDB download (left) and download of all the file jobs (right) the middle gradient colored button allows users to download the PDB with SASA values

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GlycoShape @glycoshape.org · 04/12/2025

For some unique #glycotime: C-man is a rare (but evolutionarily very old[1]) post translational modification of proteins, where the C2 of Trp in W-x-x-W/C sequons are linked to a single man through a C-C bond 🤯 This and its position within the structure stabilises the (unusual for man) 1C4 chair[2]

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GlycoShape @glycoshape.org · 02/12/2025

Tuesday #glycotime 🥄🍬 Glycans are a 🔝 example of "disordered" molecules. Inherent flexibility prevents sugars from adopting fixed, unique 3D positions relative to the crystal lattice, so they often are invisible to X-ray diffraction, unless they are bound and thus restrained in place 1/3 🧵

Example of a glycan (yellow sticks for the C atoms, blue for N, and red for O) bound to the enzyme FUT8 (1,6 fucosyltranseferase 8 in white surface) that functionalises the core with an a(1-6) fucose. The structure is also resolved with the GDP part of the donor

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GlycoShape @glycoshape.org · 01/12/2025

Today we are attending the @biochemsoc.bsky.social International Glycoimmunology Online Symposium, enjoying brilliant talks and discussions around the key role of glycans in regulating our immune system in health and disease Phenomenal #glycotime 👏👏🤩

media.tenor.com

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GlycoShape @glycoshape.org · 28/11/2025

It's Fucose Friday 🔺, the best #glycotime of the week! Fucose is commonly found as a glycan modification, but it can be linked directly to proteins as O-fucose 😎 Here ⬇️ O-fucosylation of TSR1 by POFUT2 in the ER stabilises the TSRs characteristic fold 🤩 pmc.ncbi.nlm.nih.gov/articles/PMC...

TSR type 1 structure from ADAMTS modified with a fucose by POMT2 in the ER. The fucose ring protects the disulfide bridge in yellow. Backbone in pink, C atoms of the fucose in pink and O in red.

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GlycoShape @glycoshape.org · 27/11/2025

To wish our friend and main O-glycan 3D structure contributor extraordinaire @silviadandrea.bsky.social all the best with the submission of her PhD thesis tomorrow, today's 🥄🍬 is dedicated to sialic acid 💜 with a mini 🧵 about all the wonderful functions Silvia worked on

Overview of the different roles of sialic acid (NeuAc) in glycans and glycoconjugates. Bottom left, sialic acid is represented with his chemical 3D structure (grey C atoms, red O and blue N) with a purple diamond at the back (SNFG symbol)

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GlycoShape @glycoshape.org · 26/11/2025

Today we used Re-Glyco to rebuild human EPO from AF-P01588 with glycosylation selected from Lippold et al [10.1021/acs.analchem.0c01794] From the 3D structure we identified ideal locations for extra N-glycans, some of which are actually in NESP [10.1054/bjoc.2001.1746] Super fun 3D edu #glycotime!

title slide of the workshop held today for the Advance Pharmacology module at the University of Southampton (E. Fadda lecturer and coordinator) right-hand side in the bubble, human EPO (grey) with glycosylation reconstructed according to [10.1021/acs.analchem.0c01794] bound to the EPO receptor (cyan) in PDB 1CN4. Slide background is blue

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GlycoShape @glycoshape.org · 25/11/2025

To welcome our new friends, first spoonful of sugar 🥄🍬 Glycan structures can be very complex and deceivingly similar, yet they have unique GlyTouCan IDs[1] that you can find by drawing the glycan you need in gb.glytoucan.org or in the glycoshape.org sugar drawer 1/3 🧵

Screenshot of the https://glycoshape.org front page with an arrow pointing at the Sugar Drawer tool, you cna use to search your glycan in our database

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GlycoShape @glycoshape.org · 24/11/2025

Hello bsky 🦋 GlycoShape is now here to give you updates on our glycan 3D database, toolbox, glyco fun-facts and news! We just updated server, so clear your browser's cache, and build some glycoproteins with us 🍬 We'll have great new tools coming up soon, so follow us for some exciting #glycotime

media.tenor.com

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