fatimasnc.bsky.social @fatimasnc.bsky.social · 24/03/2026Uterine "smiles"!! 🙂 🙃 🙂 Yes, you read right. Find out how and when this happens in this new preprint full of amazing images by @claireang.bsky.social et al ✨ 🔬 011
fatimasnc.bsky.social @fatimasnc.bsky.social · 06/01/2026Here's some background on our latest paper on morphogen patterning of human neural organoids! 010
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025... with special mention to our amazing collaborators @kirkebylab.bsky.social Gaurav, Pedro and Charlotte 🙌 010
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025Congratulations 👏 and huge thanks to all the authors for your essential contributions to this manuscript @akanksha-jain.bsky.social @zhisonghe.bsky.social @jasperjanssens.bsky.social @josch1.bsky.social Ryoko, Gosia, Makiko and Benedikt... 130
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025We’re so excited to share this work with the community🎉 and hope it can be used to optimise the design of the next generation of neural organoid models 🧠🧫 100
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025Something we’re particularly excited about is the co-development of neural and non-neural tissues in some of our conditions 🧩 Fine-tuning these morphogen conditions and using the right patterning modalities could help us develop more complex organoids 🏗️🏙️ 100
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025Most variability came from differences between hPSC lines and neural induction strategies, not batch or sex🧐 Dual SMAD inhibition enriched for CNS fates but was much less reproducible than minimal neural induction (without SB+Noggin) across lines🔥 100
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025Some regulons were consistent across cell lines👱🧑🦰🧑🦱 and neural induction media🧫, but others varied strongly! This gives us an idea of which cell types may be easier or harder to achieve reproducibly in vitro 🧪☑️ 100
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025By sampling organoids right after morphogen exposure, we could quantify activation of patterning regulons - signals we usually lose as neural progenitors disappear in older organoids 🫣 110
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025We sampled early-stage organoids to capture a rich spectrum of developmental states, from PSCs to neurons 👶➡️🧑 Our population of interest was the neural progenitors undergoing patterning🐣, which nicely complements other datasets focusing on more mature neuronal identities 🧠 100
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025We also designed a reproducibility screen: 12 morphogens across 4 hPSC lines, 2 neural induction media and 2 technical batches 💪 Our systematic datasets are ideal for modeling approaches 💻 like CellFlow www.biorxiv.org/content/10.1...biorxiv.orgCellFlow enables generative single-cell phenotype modeling with flow matchingHigh-content phenotypic screens provide a powerful strategy for studying biological systems, but the scale of possible perturbations and cell states makes exhaustive experiments unfeasible. Computatio... 121
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025We used SHH, WNT, FGF8, RA, and BMP4/7 pathway modulators to probe, in an unbiased way, how morphogen timing 🕑 concentration 📶 and combinations🌀affect patterning outcomes. For each morphogen, some factors matter more ➕ and others less➖! 111
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025Aware of the hurdles, we tried to put an end to this suffering 🙅♀️ Pipette in hand, @nazbukina.bsky.social and I set out to screen for as many factors as we could to better understand how early regional patterning works in human neural organoids 🧠🧫 100
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025We usually focus only on the "best" conditions⭐️ ignoring what happens beneath the surface: batch effects, mixed identities, heterogeneity 🥴 ‼️What if, instead of avoiding it, we harnessed that heterogeneity to build more complex organoid systems? 130
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025All regionalised organoid protocols rely on the same morphogens 🪅 SHH, RA, BMPs, FGFs, WNTs 🪅 applied at different doses and times. Each lab painstakingly optimises conditions to generate the purest CNS region-specific populations. 100
fatimasnc.bsky.social @fatimasnc.bsky.social · 19/12/2025Your new batch of cortical organoids resulted in mixed regional identities closer to hindbrain. Why is patterning so hard?😩 ✨Our latest paper might help you✨ tinyurl.com/neorgpat #morphogen #neuralorganoids #patterning #scRNAseq @graycamplab.bsky.social @ethz.ch @nazbukina.bsky.social 12211
Reposted by @fatimasnc.bsky.socialÇağrı Çevrim @cagricevrim.bsky.social · 10/10/2025I’m thrilled to share my postdoc work and the first paper from the McKinley Lab! 🎉 @karalmckinley.bsky.social We built the first transgenic model of menstruation in mice. We used it to uncover how the endometrium organizes and sheds during menstruation. 🧪 www.biorxiv.org/content/10.1... 🧵biorxiv.orgInduction of menstruation in mice reveals the regulation of menstrual sheddingDuring menstruation, an inner layer of the endometrium is selectively shed, while an outer, progenitor-containing layer is preserved to support repeated regeneration. Progress in understanding this co... 1423482
