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Reposted by Erh-Min Lai-IPMB

New Phytologist @newphyt.bsky.social · 02/12/2025

(🧵 8/8) By integrating floral-stage selection, immune evasion, and Agrobacterium engineering, the team established a practical platform to boost plant genetic engineering efficiency in Arabidopsis.

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Reposted by Erh-Min Lai-IPMB

New Phytologist @newphyt.bsky.social · 02/12/2025

(🧵 6/8) The team achieved several milestone discoveries for improving Agrobacterium-mediated transient expression, stable transformation, and genome-editing efficiencies in plants.

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Reposted by Erh-Min Lai-IPMB

New Phytologist @newphyt.bsky.social · 02/12/2025

(🧵 5/8) ... work to advance solutions to overcoming barriers in Agrobacterium-mediated transformation (AMT) through an Institutional Grand Challenge Project.

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Reposted by Erh-Min Lai-IPMB

New Phytologist @newphyt.bsky.social · 02/12/2025

🌱 The authors behind the paper 🌱 (🧵 4/8) A research team at the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan, led by Project Director Erh-Min Lai, with co-PIs Chih-Horng Kuo and Chih-Hang Wu...

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Reposted by Erh-Min Lai-IPMB

New Phytologist @newphyt.bsky.social · 02/12/2025

(🧵 3/8) These improvements significantly enhanced transient gene expression and CRISPR/Cas-mediated genome editing, enabling efficient recovery of edited plants without selection. This work offers a practical, high-efficiency platform to advance plant genome engineering.

Fig. 3 Agrobacterium infection and EF-Tu receptor (EFR)-mediated immunity exert negative impacts on ovule development.

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Reposted by Erh-Min Lai-IPMB

New Phytologist @newphyt.bsky.social · 02/12/2025

(🧵 2/8) In this Methods paper Liu et al. explain how they developed an optimized Agrobacterium-mediated floral transformation in Arabidopsis thaliana by identifying the most receptive floral stage and engineering agrobacterial strains to evade plant immunity.

Fig. 2 RUBY marker revealed transformation events in floral organs and female tissues.

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Reposted by Erh-Min Lai-IPMB

New Phytologist @newphyt.bsky.social · 02/12/2025

(🧵 7/8) This study was primarily conducted by co-first authors Mao-Sen Liu (Project Research Scientist) and Teng-Kuei Huang (Postdoctoral Fellow), with support from Technicians Yi-Chieh Wang and Si-Chong Wang.

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Reposted by Erh-Min Lai-IPMB

New Phytologist @newphyt.bsky.social · 02/12/2025

✨ Paper spotlight ✨ (🧵 1/8) Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis nph.onlinelibrary.wiley.com/doi/10.1111/...

Fig. 1 Agrobacterium enter open gynoecium within 1 h but transformation events occur in open flowers after 5 d post inoculation (DPI).

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Reposted by Erh-Min Lai-IPMB

Sophien Kamoun @kamounlab.bsky.social · 11/09/2025

Now we have Chih-hang Wu @IPMBSinica Taiwan 🇹🇼 at #PPATH2025 — Fast and Furious: What happens after helper NLR immune receptors are activated to cause hypersensitive cell death. See paper doi.org/10.1101/2025...

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Reposted by Erh-Min Lai-IPMB

International Society for Molecular Plant-Microbe Interactions @ismpmi.bsky.social · 14/07/2025

Connections are in action in Cologne! At #2025ISMPMI, the poster sessions aren’t just about groundbreaking research—they’re about meeting peers, collaboration, and sharing ideas that move plant-microbe science forward. Full poster hours here: bit.ly/44LqCvc #plantscience #networking

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Reposted by Erh-Min Lai-IPMB

Juan （胡安） @angiopteris.bsky.social · 14/07/2025

It was an amazing sciences day at #2025ISMPMI! Right after the “Population, biology, ecology and genomics” session, I had the opportunity to have lunch and discuss about this topic with the co-chair @honour.bsky.social

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Erh-Min Lai-IPMB @erhminlai.bsky.social · 07/06/2025

Great pleasure hosting @fuquaclay.bsky.social visiting IPMB, Academia Sinica. Enjoy scientific discussion, casual chatting over delicious meals and outing. #Agrobacterium #Taiwan

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Reposted by Erh-Min Lai-IPMB

Clay Fuqua @fuquaclay.bsky.social · 18/12/2024

Check out the ASM 10th Conference on Biofilms Bluesky page @asmbiofilms2025.bsky.social

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Reposted by Erh-Min Lai-IPMB

Alexandra Weisberg @weisbergaj.bsky.social · 20/05/2025

Happy to share a new publication on a collaboration with Xindan Wang and @fuquaclay.bsky.social labs. We identified and characterized naturally occurring Agrobacterium strains with fused chromosomes. Did mobile genetic elements play a role in fusion? journals.asm.org/doi/10.1128/...

journals.asm.org

Linear dicentric chromosomes in bacterial natural isolates reveal common constraints for replicon fusion | mBio

Most bacterial genomes are monopartite with a single, circular chromosome. However, some species, like Agrobacterium tumefaciens, carry multiple chromosomes. Emergence of multipartite genomes is often...

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Erh-Min Lai-IPMB @erhminlai.bsky.social · 13/05/2025

Happy to write this News & Views highlighting a groundbreaking study on #Agrobacterium. By combining mathematical modelling with quantitative live-cell imaging, the study uncovered a density-dependent synergistic and antagonistic effect on #AMT

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Yi-Chang Sung （宋宜璋） @yichangsung.bsky.social · 25/04/2025

Our Research Briefing on the tandem kinase proteins paper is online now! Curious about the story behind our TKP paper? 📄 Research Briefing: www.nature.com/articles/s41... 📄 Research article: www.nature.com/articles/s41...

nature.com

A plant dual-kinase protein traps a pathogen effector to trigger immunity - Nature Genetics

Tandem kinase proteins (TKPs) are key regulators of plant immunity. We found that RWT4, a wheat TKP, confers resistance to the fungal pathogen Magnaporthe oryzae by directly recognizing the effector A...

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Reposted by Erh-Min Lai-IPMB

Ching-Jung Lin @chjlin.bsky.social · 09/05/2025

👀 EFR-mediated PTI restricts transient transformation and genome editing, but not T-DNA integration. This study combines floral stage targeting 🌷, immune evasion 🛡️, and Agrobacterium engineering 🔧 to boost plant genome editing efficiency. #Agrobacterium #GenomeEditing www.biorxiv.org/content/10.1...

biorxiv.org

Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis

Agrobacterium-mediated transformation via floral inoculation (AMT-FI) enables genetic engineering without tissue culture. It is widely used in the model plant Arabidopsis thaliana, yet its efficiency ...

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Reposted by Erh-Min Lai-IPMB

Gitta Coaker @gittacoaker.bsky.social · 09/05/2025

Nice preprint from Erh-Min Lai's lab. "By integrating floral stage selection, immune evasion, and Agrobacterium engineering, this study provides a practical and versatile platform to advance plant genome engineering." vhttps://www.biorxiv.org/content/10.1101/2025.05.07.652770v1

biorxiv.org

Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis

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Reposted by Erh-Min Lai-IPMB

Sophien Kamoun @kamounlab.bsky.social · 09/05/2025

Tweeps, please spread the word. We're still looking at recruiting a structural biologist (any level) to study plant-pathogen protein complexes especially in the context of coevolution. Direct inquiries welcome. Please include a cover letter. Applicant from US welcome? medium.com/@kamounlab/2...

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