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Emmanuel Beaurepaire

@ebeaurepaire.bsky.social
115 followers 46 following 37 posts

Biomicroscopist @laboptbio.bsky.social @CNRS.bsky.social Polytechnique lob.ip-paris.fr

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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 13/10/2026
Like the classical paradox: are you a liar ?
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 13/10/2026
This is a good criterion, but what would an arrogant person answer to that question ? 🤔
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AIP Publishing @aip-publishing.bsky.social · 07/10/2026
This study shows that third-harmonic generation (THG) is enhanced in a wavelength-dependent manner by biological pigments. This mechanism can be used to detect absorbing structures in THG microscopy. @laboptbio.bsky.social @cnrs.fr @inserm.fr @ipparis.bsky.social 🔗 aippub.org/4dNQCv4
pubs.aip.org
Resonant third harmonic generation in biological pigments
Third harmonic generation (THG) microscopy provides label-free structural contrast of biological tissues. While the THG process can be enhanced by electronic tr
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Reposted by Emmanuel Beaurepaire
Rob Campbell @raacampbell.com · 04/10/2026
Preprint is out! Our BakingTray software turns a 2-photon microscope into a serial-section tomography system for automated whole-organ imaging. ~€35k to convert an existing scope. 11 systems worldwide, >7,000 samples at SWC alone #neuroskyence #microscopy www.biorxiv.org/content/10.6...
A BrainSaw at the Max Planck Institute for Brain Research, Frankfurt. A Nikon 16x water-immersion objective on a 400 µm travel PIFOC allows for fast z-stacks. Samples are imaged and cut in the clear water bath. A Leica VT1000 vibratome is seen to the right with a mounted blade.How BrainSaw works. Samples are embedded in an agarose block, which is glued to a slide and clamped into a water bath. The bath sits on a three-axis stage under a 2-photon microscope; the stage moves the sample for both tile scanning and slicing. The microscope images the exposed face of the block, the vibratome cuts off the imaged layer, and the cycle repeats until the samples have been fully imaged. Data are stitched automatically and are ready for downstream analysis. Logos below: BrainSaw (hardware), BakingTray (acquisition software), StitchIt (stitching).Coronal section of an Sst-tdTomato mouse brain imaged on BrainSaw. tdTomato-expressing cells are shown in yellow, scattered through cortex and hippocampus; tissue autofluorescence is shown in blue.Coronal section of a mouse brain with three neighbouring viral injections targeting layer 5 of V1, expressing mCherry (red), eGFP (green) and BFP (blue). The thick apical dendrites of the labelled neurons are clearly visible, as are their axons projecting to distant targets. Image credit: Alex Fritzl.
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 12/09/2026
@stelladees.bsky.social et al.'s work on THG in pigments is featured in #Scilight @aip.bsky.social 🔹 doi.org/10.1063/10.0...
doi.org
A label-free imaging technique can distinguish biological pigments
The method identifies tiny interfaces within a material by monitoring variations in refractive index and absorption, making it sensitive to pigments.
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 04/09/2026
A nice study by @laboptbio.bsky.social colleagues V.Abdelsayed, P.Mahou,G.Latour,MC.Schanne-Klein and collaborators in J.Ramella-Roman's group at FIU. They use polarized SHG to analyze the reorganization of cervix collagen matric during gestation in mice. Congrats Vaky & co! doi.org/10.1063/5.03...
doi.org
Advanced optical polarimetric microscopy reveals spatio-temporal dynamics of cervix remodeling during gestation
Polarimetric microscopy is a powerful tool for assessing fiber orientation and reorganization during various biological processes. Collagen fiber remodeling in
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 25/08/2026
-> Wavelength-resolved THG appears to be an interesting approach for pigment-sensitive in situ imaging. Congrats @stelladees.bsky.social and co-authors @juliaferrerortas.bsky.social #PierreMahou @wsupatto.bsky.social #NicolasOlivier ! Thanks funders @erc.europa.eu @agencerecherche.bsky.social
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 25/08/2026
We find that all of these pigments exhibit resonances resulting in strong, wavelength-dependent THG responses. We also show that third-order sum-frequency generation (TSFG) imaging using a dual-output source produces "color THG" images that distinguish different structures. (...)
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 25/08/2026
This property could be used for selective imaging, but it is underutilized because it is poorly characterized in biological contexts. In this study, we analyze the THG spectra of 3 pigments: hemoglobin in red blood cells, pteridines in zebrafish xanthophores, and melanin in human hair. (...)
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 25/08/2026
🔬🌟 Third-harmonic generation (THG) microscopy is a label-free 3D biological tissue imaging technique tissues that can be combined with multiphoton fluorescence. While THG images are mostly used to get morphological information, the THG process can be enhanced in the presence of absorbers. (...)
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 25/08/2026
Resonant third harmonic generation in biological pigments Dees et al, APL Photonics 2026 doi.org/10.1063/5.03... A new article from @laboptbio.bsky.social microscopy unit @cnrs.fr @inserm.fr @xpolytechnique.bsky.social @ipparis.bsky.social (1/5)
doi.org
Resonant third harmonic generation in biological pigments
Third harmonic generation (THG) microscopy provides label-free structural contrast of biological tissues. While the THG process can be enhanced by electronic tr
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 10/08/2026
well done! what about your cell phone ? 😄
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Laboratory for Optics and Biosciences @laboptbio.bsky.social · 10/06/2026
A new paper from the LOB microscopy unit on high-speed multiphoton light-sheet imaging has been published in Optica. We achieved kilohertz frame rates by enhancing the signal-to-photodamage ratio during live imaging. doi.org/10.1364/OPTI...
doi.org
Radware Captcha Page
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Lei.ZHU @leizhu.bsky.social · 05/06/2026
🚀 KiloHz two photon live imaging #MultiphotonMicroscopy @laboptbio.bsky.social Two-photon light-sheet live imaging at kilohertz frame rate using birefringence-based pulse splitting #LightSheetMicroscopy #Bioimaging #Photonics #Microscopy #Zebrafish Article is available here: doi.org/10.1364/OPTI...
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Laboratory for Optics and Biosciences @laboptbio.bsky.social · 07/04/2026
Caroline Boudoux, Professor at @polymtl.bsky.social and entrepreneur will give the next LOB seminar: "It Goes without Saying: Taking the Guesswork of Your PhD in Engineering" See you on April 17th at 11AM in Amphi Becquerel. lob.ip-paris.fr/en/events/se...
lob.ip-paris.fr
Seminar of Caroline Boudoux - Professor at Polytechnique Montréa et an entrepreneure (Castor Optics)
11:00am, Ecole Polytechnique, Amphi Becquerel
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Laboratory for Optics and Biosciences @laboptbio.bsky.social · 25/03/2026
An INSERM CHAIR (tenure track position) has just been opened in the Laboratoire d'Optique et Biosciences (LOB) (INSERM U1182) in @xpolytechnique.bsky.social / @ipparis.bsky.social @inserm.fr Deadline to apply : September 2. pro.inserm.fr/wp-content/u...
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 22/03/2026
😄
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 14/02/2026
oh, nice 👍
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Chiara Stringari @chiarastringari.bsky.social · 07/01/2026
Our paper “Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue” is published in @commsbio.nature.com, thanks to an exciting collaboration between @laboptbio.bsky.social and @institutducerveau.bsky.social www.nature.com/articles/s42...
nature.com
Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue - Communications Biology
Multimodal label-free nonlinear microscopy and advanced image analysis map single-cell metabolism and myelin dynamics in live cerebellar tissue during demyelination, supporting research in neuroinflam...
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Laboratory for Optics and Biosciences @laboptbio.bsky.social · 09/10/2025
(2025/10/08) Congratulations to @chiarastringari.bsky.social on the defense of her Habilitation à Diriger les Recherches (HDR) "Label-free multimodal non-linear optical microscopy for functional imaging of living tissues" !
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 16/09/2025
I first understood that you published with your husband and your wife - separate papers 😂
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Nicolas Chiaruttini @nkiaru.bsky.social · 24/07/2025
Hey, it's out! doi.org/10.1016/j.ce... Aligning Big Brains and Atlases, ABBA in short, is published in Cell Reports @cp-cellreports.bsky.social . @biancasilvalab.bsky.social's lab added an analysis pipeline on top of it (BraiAn) and did all the relevant biological work. What is it ? Short thread:
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 18/07/2025
petroleum 😅
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 16/07/2025
Happy to finally publish this work… Congrats authors and thanks funders @agencerecherche.bsky.social #FondationBettencourtSchueller‬‬‬
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 16/07/2025
These findings help to establish THG microscopy as a quantitative, multiscale imaging technique, paving the way for new applications. 📖 #Neuroscience #Myelin #THG #Microscopy #Research
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 16/07/2025
Finally, we illustrate the relevance of pTHG microscopy for characterizing myelin distribution at different scales in fixed mouse and human brain tissue.
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 16/07/2025
Fun fact for multiphoton microscopy geeks: aberrations caused by vertical micro-interfaces appear to be a significant contrast mechanism in polarized THG imaging. 😎
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 16/07/2025
We show that axons exhibit complex pTHG profiles that depend on axon morphology and can't be explained by classical models. We establish a numerical strategy to relate pTHG profiles to axon diameter and myelin thickness, taking into account myelin-induced optical aberrations.
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 16/07/2025
Our study presents a systematic characterization of polarized THG (pTHG) signals from individual axons, enabling the derivation of myelin scores in various systems. 🐭🐟 We confirm the sensitivity of THG for myelin in mouse tissue and detect early axon development in zebrafish.
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 16/07/2025
By #J_Morizet #N_Olivier #MS_Aigrot #P_Mahou #E_Martin #A_Desmazieres #B_Stankoff @chiarastringari.bsky.social 🔬🌟 Find out how Third Harmonic Generation (THG) microscopy could help advance myelin biomedical research! 🧠
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 16/07/2025
Multiscale characterization of myelin distribution with polarized THG microscopy Morizet et al, Optica 2025 doi.org/10.1364/OPTI... @laboptbio.bsky.social @institutducerveau.bsky.social @cnrs.fr ‪‪ @ip-paris.bsky.social @inserm.fr (1/8)
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Laboratory for Optics and Biosciences @laboptbio.bsky.social · 01/07/2025
Congratulations to @caporalclement.bsky.social on his brilliant defence of his PhD thesis, entitled 'Quantitative Analysis of Large Volume Imaging in Developmental Neurobiology' !
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 25/06/2025
divergence
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Brice Bathellier @bathellierlab.bsky.social · 15/06/2025
Two postdoc positions in the lab within the ERC Synergy project "Chronology" in collaboration with S. Ostojic, M. Jazayeri and V. van Wassenhove @virginievanw.bsky.social . Two focuses: sound sequences representations across the brain & novel all-optical technology. Contact: brice.bathellier@cnrs.fr
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 07/06/2025
movie theaters :)
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 07/06/2025
+1
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Ignacio Arganda-Carreras @iarganda.eurosky.social · 13/05/2025
🆕 We're hiring! We're looking for postdoc 🎓 candidates to work at the intersaction of bioimage analysis 🖥️🔬, tissue biomechanics 🧫, and neuroscience 🧠. 👉 Application deadline: 2025/06/02 👉 Duration: July 2025 - December 2026 (for now) Come work with us! 🤗 dipc.ehu.eus/en/dipc/join...
dipc.ehu.eus
Joint Postdoctoral Position in Biophysics – Bioimage Analysis / AI / Neuroscience
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Laboratory for Optics and Biosciences @laboptbio.bsky.social · 09/04/2025
Congratulations to Stella Dees for defending her thesis on "Multicontrast 3P-microscopy for biological tissue imaging"
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Laboratory for Optics and Biosciences @laboptbio.bsky.social · 07/04/2025
Congratulation to Giulia Galante for her thesis defense about the "Characterization and physico-chemical monitoring of collagen degradation in parchments by polarization-resolved multiphoton microscopy!
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Chiara Stringari @chiarastringari.bsky.social · 13/02/2025
See you on Friday February 21st for the @eurobioimaging.bsky.social Virtual Pub on "Fluorescence Lifetime Microscopy (FLIM) for label-free metabolic imaging of living tissues"! #FLIM #labelfree #microscopy #metabolism You can register online here! www.eurobioimaging.eu/events/flim-...
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Chiara Stringari @chiarastringari.bsky.social · 13/02/2025
Do you need to analyze your FLIM data with open source software? The #Virtual #I2K2024 workshop on "Interactive #phasor analysis of #FLIM data using FLUTE" given by Bahar Asadipour is online. www.youtube.com/watch?v=q7W1... #FLUTE can analyze data of most commercial cards github.com/LaboratoryOp...
youtube.com
Interactive phasor analysis of FLIM data using FLUTE
YouTube video by I2K Conference
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 22/01/2025
True, but it is also a matter of disseminating scientific knowledge. Biology has made tremendous and rapid progress in the last century, with consequences for medicine. (So has physics (not to mention computer science 😂)).
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 22/01/2025
Un poste d’ingénieur en analyse d’images est ouvert en mobilité pour rejoindre @laboptbio.bsky.social à @polytechniqueparis.bsky.social (Palaiseau). Poste IR ouvert aux IE de la fonction publique et IR @cnrs.bsky.social. Deadline: 20/02. Lien (concours 508): profilsdemplois.cnrs.fr/ords/afipprd...
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 22/01/2025
An image analysis engineer position is open for mobility to join @laboptbio.bsky.social at @polytechniqueparis.bsky.social ky.social in Palaiseau. IR position open to civil service IEs and CNRS IRs. Deadline: 20/02. Link (position #508): profilsdemplois.cnrs.fr/ords/afipprd...
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 21/01/2025
The natural sciences feed into the human sciences, which gradually integrate the discoveries made in biology. It just takes a while... Now think about religions :)
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 14/01/2025
That said, it's a nice model of a scattering & aberrating medium 🤓
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 14/01/2025
Is this your comfort food? 😬😬
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 13/01/2025
not so easy, to be honest... sweet? fat? liquid?
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Emmanuel Beaurepaire @ebeaurepaire.bsky.social · 13/01/2025
scale bar ?
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