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Clément Madru

@cryoclem.bsky.social

117 followers 130 following 12 posts

Assistant Professor in structural biology (💎 & 🔬) Structural Biology of the Cell - BIOC École polytechnique - Institut Polytechnique de Paris - Palaiseau -France.

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Clément Madru @cryoclem.bsky.social · 12/06/2026

Merci @xpolytechnique.bsky.social pour le coup de projecteur sur notre étude ! 🔬 Comment les ribosomes des archées hibernent-ils ? Réponse ici :

polytechnique.edu

Comment les ribosomes des archées « hibernent » - École polytechnique, éco…

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Clément Madru @cryoclem.bsky.social · 05/05/2026

Happy to share our latest paper: www.nature.com/articles/s41... Great collaboration with @ifremer.bsky.social, @cbitoulouse.bsky.social & @pasteur.fr. Studying stress in P. abyssi with near-native, minimally purified ribosomes, we identified HibA, a new hibernation factor widespread in Archaea.

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Clément Madru @cryoclem.bsky.social · 15/01/2026

Sharing this figure from the Koonin lab: www.nature.com/articles/s41... Worth printing large and framing

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Clément Madru @cryoclem.bsky.social · 13/10/2025

Thank you Olivier ! In a stressed P. abyssi culture, almost 50%. It does not translocate, the C-ter remains locked in the decoding center in all 3 conformations. It's unclear how Hib, or other hibernation factors are released..

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Clément Madru @cryoclem.bsky.social · 13/10/2025

5/🧵 This work is the result of a great collaboration between labs at @ipparis.bsky.social , @ifremer.bsky.social , @cbitoulouse.bsky.social and @pasteur.fr Many thanks to all colleagues involved 🙌

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Clément Madru @cryoclem.bsky.social · 13/10/2025

4/🧵 - In one of these conformations, Hib blocks the peptidyl transferase center (PTC) of the large subunit by occupying both the A and P sites : A long Hib loop encircles nucleotide A2834, which separates the P and A sites, effectively locking the ribosome in an inactive state

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Clément Madru @cryoclem.bsky.social · 13/10/2025

3/🧵- High-resolution cryo-EM structures of reconstituted and in cell-extracted Hib:ribosome complexes from Pyrococcus abyssi identify three conformations encompassing the positions of tRNAs at A, P and E sites during translation.

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Clément Madru @cryoclem.bsky.social · 13/10/2025

2/🧵 - Hib adds to the growing list of archaeal ribosome hibernation factors, alongside recently identified Dri and aRDF. It is detected in all major archaeal lineages, representing about 47% of all genomes.

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Clément Madru @cryoclem.bsky.social · 13/10/2025

Our work on ribosome hibernation in archaea is out! We identified Hib, a new hibernation factor broadly distributed across archaea. Check out the preprint 👉 biorxiv.org/content/10.1101/2025.10.11.676729v1 (1/🧵)

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Clément Madru @cryoclem.bsky.social · 30/01/2025

yep ! and with little biomass

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Clément Madru @cryoclem.bsky.social · 30/01/2025

First ribosome purification using poly-lysine beads (RAPPL, developed by @djuranoviclab.bsky.social & collaborators: www.biorxiv.org/content/10.1...). Full process—from lysis to grid prep—in just 3 hours. Look how pretty it is (even if a bit too diluted)! This is a game-changer🚀.

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Clément Madru @cryoclem.bsky.social · 18/01/2025

A new year, a new social network and a new article in @naturecomms.bsky.social ! It unveils how archaeal RPA orchestrates the recruitment of key players in 🧬 replication primase and polD through its WH domain, with structures of both complexes!! rdcu.be/d6GAY

rdcu.be

Communication between DNA polymerases and Replication Protein A within the archaeal replisome

Nature Communications - The single stranded DNA binding protein RPA plays a pivotal role in DNA replication. The archaeal RPA hosts a WH domain that interacts with the DNA primase and the...

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Reposted by Clément Madru

Schmitt-Mechulam Lab @tbioc.bsky.social · 16/01/2025

We are pleased to share our work on the evolution of translation initiation now published in Nature Communications. Take a look at leaderless mRNAs and eS26 in the small ribosomal subunit of Saccharolobus solfataricus. rdcu.be/d5pgg

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