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AyalaLab

@ayalalab.bsky.social
58 followers 51 following 21 posts
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PLOS Biology @plosbiology.org · 26/03/2026
In some #neurodegenerative diseases, TDP-43 is both lost from the nucleus & forms clumps in the cytoplasm. These two pathologies can be challenging to model but a study in @plosbiology.org presents a new system that captures both features 🧪 Paper: plos.io/4sxw0g9 Primer: plos.io/4bvWGrw
A cell-based reporter of TDP-43 disease states. Top left: In healthy cells, TDP-43 is predominantly nuclear. Within the nucleus, TDP-43 plays important roles in repressing the inclusion of cryptic exons as well as regulating its own transcript levels and isoforms. This ensures appropriate levels of functional TDP-43 within the cell. Top right: In disease, TDP-43 aggregates in the cytoplasm and is lost from the nucleus, leading to both gain-of-function and a loss-of-function. Loss of nuclear function leads to multiple detrimental events. These include DNA damage as well as the inclusion of cryptic exons in transcripts (depicted as orange boxes in the diagram), in turn leading to the production of incorrectly processed transcripts. In addition, disrupted TDP-43 autoregulation leads to unchecked accumulation of the protein’s own transcript. Bottom left: The reporter line developed by Mamede and colleagues enables the study of TDP-43 aggregation in response to stimuli—in this case exposure to TDP-43 protein aggregates that originated from patients with FTD. The reporters are composed of the C-terminal domain of TDP-43 fused to either a green or red fluorescent protein (FRET pairs). When the reporter aggregates, the proximity of TDP-43 FRET pairs enables the quantitative evaluation of aggregation within each cell. Bottom right: Protein aggregates can be quantified after excitation using a blue laser. Cells containing TDP-43 aggregates produce a FRET-positive signal that can be measured using flow cytometry whilst cells that do not have aggregates do not produce a signal. Image created in BioRender.
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Reposted by AyalaLab
PLOS Biology @plosbiology.org · 26/03/2026
TDP-43 is implicated in #neurodegeneration, but how does its aggregation relate to dysfunction? @ayalalab.bsky.social &co develop a reporter & cellular model based on #TDP-43 aggregate seeding that captures both TDP-43 aggregation & loss of protein function @plosbiology.org 🧪 plos.io/4sxw0g9
Disease-derived extract strongly activates TDP-43 aggregate seeding in a sensitive FRET-based biosensor. Top: The HEK293FRET cell line stably expressing the TDP-43 C-terminal domain (CTD, amino acids 262-414) fused to mClover3 and mRuby3 FRET pairs was used as a reporter of TDP-43 aggregation upon seeding with FTD extract, prepared as in S1A Fig. Image created in BioRender, Ayala, Y. (2026). Middle: de novo CTD aggregation in HEK293FRET cells treated with FTD seeds visualized by confocal fluorescence microscopy. As control, cells were treated with neurologically unaffected tissue extract. Bottom: High resolution images of aggregates in cells treated with FTD seeds as z-stacks with DAPI overlay generated with stimulated emission depletion (STED) microscopy. The white box corresponds to the enlarged region. Scale bar for microscopy images, 10 μm.
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Reposted by AyalaLab
Jeremy Berg @jeremymberg.bsky.social · 29/07/2025
The impacts are becoming apparent as institutes settle on new “paylines,” or the portion of peer-reviewed grants that meet the cutoff for funding. The dramatic drop at NCI is likely to result in “substantial loss of Pis and staff,” NCI Deputy Dir. Douglas Lowy recently told an advisory board. 3/n
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AyalaLab @ayalalab.bsky.social · 29/07/2025
Odds of winning NIH grants plummet as new funding policy and spending delays bite | Science | AAAS www.science.org/content/arti...
science.org
Odds of winning NIH grants plummet as new funding policy and spending delays bite
Funding multiyear grants up front will sharply cut number of investigators receiving awards
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Reposted by AyalaLab
Dr. David Miller 🏳️‍🌈 @davidimiller.bsky.social · 02/07/2025
🧵 3/3 Bottom line for 🧪: The last remaining fight over the Billionaires' Bill is this week (*right now*) and in the House. The fight for science funding in FY26 starts *next week* in the House and will continue during the summer. So: don't lose hope...it's a *summer-long* fight for science!
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Reposted by AyalaLab
American Society for Cell Biology @ascbiology.bsky.social · 01/07/2025
The "Big Beautiful Bill" isn’t the federal budget—it’s a reconciliation bill focused on tax cuts. The real FY26 funding decisions for NIH, NSF, and more are happening now in Congress. www.ascb.org/science-poli...
ascb.org
The "Big Beautiful Bill" is Not the Budget - ASCB
If you’ve read recent news about Congress trying to pass “the budget,” you may have noticed that even Washington, D.C. reporters don’t always have a firm grasp on the details of what they’re covering....
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AyalaLab @ayalalab.bsky.social · 29/06/2025
Call your legislators to stop slashing the budget of federal funded research in the “Big Beautiful Bill”. Find numbers @5calls.org
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Reposted by AyalaLab
Stand Up for Science! @standupforscience.net · 29/06/2025
The proposed budget slashes NIH by ~40%, devastating medical research, clinical trials, treatment breakthroughs—life-saving work. Thousands of lives at risk for tax cuts for the rich? NO THANKS. Tell your senators to oppose the cuts HERE: zurl.co/qiKdX zurl.co/RCR0y #CutsCostLives
zurl.co
Will the Senate Save NIH?
Rescuing biomedical research is every bit as important as saving Medicaid.
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Jeremy Berg @jeremymberg.bsky.social · 13/05/2025
New report from Bernie Sanders, ranking member on the HELP committee www.sanders.senate.gov/wp-content/u... Lots of substance in here. Some highlights... 1/n
sanders.senate.gov
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Reposted by AyalaLab
Brenton Graveley @graveley.bsky.social · 17/04/2025
Bad, bad news for health and research in the US. Cuts to NIH are devastating
washingtonpost.com
Internal budget document reveals extent of Trump’s proposed health cuts
HHS would be asked to absorb a $40 billion cut, about one-third of its discretionary budget.
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AyalaLab @ayalalab.bsky.social · 15/04/2025
“If Harvard had not taken this stand,…it would have been nearly impossible for other institutions to do so.” www.nytimes.com/2025/04/14/u...
nytimes.com
Harvard’s Decision to Resist Trump Is ‘of Momentous Significance’
But a fight with the nation’s oldest, richest and most elite university is a battle that President Trump and his powerful aide, Stephen Miller, want to have.
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AyalaLab @ayalalab.bsky.social · 13/04/2025
www.nytimes.com/2025/04/12/u...
nytimes.com
Harvard Professors Sue Trump Administration Over Threat to Federal Funds
The administration is reviewing about $9 billion in federal funding that the university receives.
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AyalaLab @ayalalab.bsky.social · 02/04/2025
It happened to a loved one today 😭, a person who worked tirelessly to protect you and me. This is the thanks they get
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Reposted by AyalaLab
MaRtiNA Hallegger @martinahallegger.bsky.social · 01/04/2025
Very excited and grateful to get this opportunity to study new aspects of TDP-43 with @nicolasluxfawzi.bsky.social @ayalalab.bsky.social and Jeetain Mittal!
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AyalaLab @ayalalab.bsky.social · 20/03/2025
www.stltoday.com/news/local/m...
stltoday.com
Study shows drug may prevent Alzheimer’s. NIH slowdowns threaten future research
Washington University-led research shows a small group of participants in an Alzheimer’s study experienced a significant delay in the onset of symptoms.
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AyalaLab @ayalalab.bsky.social · 19/03/2025
On March 14, Senate passed the Continuing Resolution cutting CDMRP funds. It’s devastating to scientific progress and neurodegenerative disease research including ALS. Ask the Department of Defense and Congress to restore funding for ALS CDMRP funds: alsnetwork.quorum.us/campaign/11362…
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Reposted by AyalaLab
Matt Kertman @mattkertman.bsky.social · 08/03/2025
Scenes from the incredible turnout today at Berkeley's @standupforscience.bsky.social rally. @tedmiguel.bsky.social, @jenniferdoudna.bsky.social, and dozens of researchers, elected officials, and citizens defended science and the jobs, health benefits, and progress it creates for all Americans.
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AyalaLab @ayalalab.bsky.social · 07/03/2025
Yay signs in #standupforscience2025
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AyalaLab @ayalalab.bsky.social · 07/03/2025
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AyalaLab @ayalalab.bsky.social · 07/03/2025
Today is the day. #standupforscience2025 Protect research, science saves lives www.eventbrite.com/e/stand-up-f...
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the beggars out of here getter @milesklee.bsky.social · 05/03/2025
Trump claimed the Biden administration spent $8 million "making mice transgender." The White House tried to back up this number with records of funding for research into cancer, asthma, and HIV, some of which used *transgenic* (genetically modified) mice.
rollingstone.com
Trump Decried Millions Spent 'Making Mice Transgender.' It Was Cancer and Asthma Research
President Trump falsely claimed that Biden spent $8 million on 'making mice transgender,' but the real research was for human health.
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Celeste Karch @karchlab.bsky.social · 06/03/2025
If you are in St Louis this weekend, join MO Jobs with Justice to support federal workers
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Jamie Bear 🐻 @jamiebear.bsky.social · 06/03/2025
If you’re in DC, the slate of speakers for this event on Friday is solid. I’ll be there.
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Reposted by AyalaLab
Scott McGrath @smcgrath.phd · 06/03/2025
🧪 Idaho, Wyoming, North and South Dakota - time to get organizing, it is not too late! Let's get this in all 50 states!
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Mara Duncan @mcduncanlab.bsky.social · 06/03/2025
Hey Michiganders, This Friday in Lansing many scientists and concerned citizens will gather to stand up for science at noon. Come and show that Michiganders support science, and value the constitutional rights to free speech and peaceful assembly. standupforscience2025.org/local-event-...
A poster for stand up for science. Lady liberty stands in front of red banners saying Stand up for Science and Science is for everyone.

The background reads standupforscience2025.org March 7, 2025
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Tim Mosca (he/him) @drosophilosophy.bsky.social · 05/03/2025
Just a reminder! Friday morning! Hope to see many of my Philly folk there!
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Rachel @rachelf22.bsky.social · 01/03/2025
📢 Atlanta! Time to stand up for science! Why? Because science is for everyone. Join us on 3/7 from 12-4 at Liberty Plaza- Georgia State Capital @standupforscience.bsky.social
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Todd Douglas @apoptoddic.bsky.social · 01/03/2025
Connecticut friends -- we're one week away! I hope to see you at the Capitol in Hartford on March 7. Let's bring some light to these dark times and stand up for science. @standupforscience.bsky.social @mcdb-yale.bsky.social
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Stand Up for Science! @standupforscience.net · 26/02/2025
Joining the DC or your local Stand Up for Science event, or just want to learn how scientists can safely engage in advocacy? ☀️ Join this Union of Concerned Scientists event tonight from 8:00–9:30 p.m. ET - secure.ucsusa.org/a/2025-02-26... *not a sponsored post, just sharing out of interest!
secure.ucsusa.org
Protecting Scientists Engaging in Advocacy
The Union of Concerned Scientists invites you to a webinar to help scientists and experts understand their first amendment rights, enhance their digital privacy, and navigate the risks involved in eng...
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Kal S. @kalgayming.bsky.social · 26/02/2025
There’s a branch of this (unaffiliated, but same message) being planned at RIT in Rochester , NY (3 hours from our capital in Albany) so that the density of students in western New York can also take part. 12pm walkout!
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AyalaLab @ayalalab.bsky.social · 22/02/2025
Scientists warn of long-term damage as Trump’s orders slow research
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memyself1.bsky.social @memyself1.bsky.social · 22/02/2025
Help our Science friends out if you are in these cities please!
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AyalaLab @ayalalab.bsky.social · 22/02/2025
Jefferson City, MO March 7, 1-4pm Protect Research, Science Saves Lives
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Celeste Karch @karchlab.bsky.social · 22/02/2025
In January, we were closer than ever before to finding life changing treatments for Alzheimer’s disease. The funding stoppage has slowed this progress.
nytimes.com
Trump Administration Stalls Scientific Research Despite Court Ruling
Using an arcane law, officials have effectively delayed funding from the National Institutes of Health, leaving medical studies in jeopardy.
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David Grinspoon @drfunkyspoon.bsky.social · 19/02/2025
Stand up for Science: March 7 🧪 standupforscience2025.org
standupforscience2025.org
STAND UP FOR SCIENCE
March 7, 2025. Washington DC and Nationwide. Because Science is for everyone.
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Stand Up for Science! @standupforscience.net · 20/02/2025
☀️📣 Looking for a STATE LEAD and VOLUNTEERS to help us #standupforscience2025 in Illinois! Know someone interested? Fill out this form: airtable.com/app4h5wUssMG... #scienceforall #sciencenotsilence
photo of illinois with text looking for illinois volunteers
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Stand Up for Science! @standupforscience.net · 20/02/2025
☀️📣 Looking for a STATE LEAD and VOLUNTEERS to help us #standupforscience2025 in Kansas! Know someone interested? Fill out this form: airtable.com/app4h5wUssMG... #scienceforall #sciencenotsilence
Photo of kansas with text, looking for kansas volunteers
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Brittany Barker @brittanysbarker.bsky.social · 20/02/2025
@standupforscience.bsky.social repost! #standupforsciemce #sciencenotsilence #waronscience
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The Atlantic @theatlantic.com · 15/02/2025
For decades, the U.S. has funded science, then left scientists to direct their own work. Science has flourished, and the country has benefited. Now the government is testing just how much it can renegotiate that relationship, @katherinejwu.com writes:
theatlantic.com
The Erasing of American Science
How far can the Trump administration bend U.S. research before it breaks?
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Kate Starbird @katestarbird.bsky.social · 08/02/2025
Scientists and journalists need to figure out right quick how to explain to the average person how a massive change in research indirects will impact the medical care they and their children get (eg at the local children’s hospital), the education their children will get, the price of tuition, etc.
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Stand Up for Science! @standupforscience.net · 16/02/2025
📣 DC friends - please consider supporting this rally on Wednesday, February 19th!
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Jeremy Berg @jeremymberg.bsky.social · 16/02/2025
Thanks to all of the NIHers and their friends who reached out to me. I am still here (DM me or Signal jeremymberg.78) I still have a very incomplete picture but based on what I have been told, the damage to NIH and to many wonderful people who work(ed) there is/was impossible for me to imagine 1/n
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Benjamin Riley @benjaminjriley.bsky.social · 16/02/2025
Fighting to stop what's happening is profoundly patriotic, profoundly American. And We the People are mobilizing on March 7 to stand up for science. To be light in the darkness. @standupforscience.bsky.social
Uncle Sam poster, he wants you to stand up for science.
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Brian Trainor @briantrainor.bsky.social · 16/02/2025
The sheer incompetence in the way all of this is being done is my only hope that some of this can be undone. We’re going to a town hall for our congressman to raise some hell. I’m tired of going to voicemail
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AyalaLab @ayalalab.bsky.social · 16/02/2025
www.nytimes.com/interactive/...
nytimes.com
How Trump’s Medical Research Cuts Would Hit Colleges and Hospitals in Every State
Changes to a key funding formula will reduce research grants at hospitals and universities by billions — and may discourage future research.
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Katie Langin @klangin.bsky.social · 14/02/2025
Dear federal scientists: If you have been affected by the probationary firings and would be willing to speak with Science, please reach out to me by email (klangin@aaas.org) or on Signal. We can guarantee annonymity.
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Jeremy Berg @jeremymberg.bsky.social · 14/02/2025
(Sorry I took a brief timeout for uncontrollable weeping) In other NIH news, I have heard from multiple sources that NIH plans to fire most, if not all, probationary employees this afternoon. The numbers I am hearing are >5000. Lots of folks extramural staff, intramural scientists, etc.
media.tenor.com
a man with glasses and a mustache is making a sad face
ALT: a man with glasses and a mustache is making a sad face
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Mike Feigin 🥯 @mikefeigin.bsky.social · 14/02/2025
We should all be taking to the streets. March 7. DC, state capitols, other big cities. Go to the website and sign up. Honestly, what the hell else will it take?
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Mark Peifer (He, him) @peiferlabunc.bsky.social · 13/02/2025
Take action for science. Great to see my favorite scientific society give us the tools we need. Another tool is the 5 Calls app that identifies the members of congress & provides top issues & a script along with phone numbers 🧪 5calls.org
5calls.org
5 Calls
Spend 5 minutes. Make 5 calls. Make your voice heard.
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American Society for Cell Biology @ascbiology.bsky.social · 13/02/2025
NIH-funded research is at risk. Cuts to federal funding could harm science and the economy. Contact Congress now to protect research. Every call matters. Find your reps at govtrack.us and speak up today. #FundScience www.ascb.org/science-poli...
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