New lab paper in @natcomms.nature.com!!! With many replicates in long-read RNA-seq, do you pool reads before calling transcripts (Join & Call) or call per sample and merge (Call & Join)? We tested both with 6 tools, PacBio & ONT. It depends on the tool!
doi.org/10.1038/s414...
doi.org
Handling biological replicates in long-read RNA sequencing data by joining or not joining - Nature Communications
When combining long-read RNA sequencing data from multiple samples, transcripts can be identified either before or after merging. This study shows that neither approach is universally optimal and prov...