Methionine is an essential amino acid, used for protein synthesis, redox homeostasis, and methylation reactions throughout the cell. However, the compartmentalized dynamics of methionine have remained...

Abstract. AMP-activated protein kinase (AMPK) is a crucial regulator of cellular energy balance, affecting numerous downstream targets across various subcellular locations. Under cellular energy stres...

Adaptations to changing environments manifest in various cellular activities across multiple timescales, where single-cell responses can occur asynchronously between individual cells. Hence, accurate ...

Phosphatase and tensin homologue-induced kinase 1 (PINK1) is a serine/threonine kinase that plays roles in mitophagy, cell death, and regulation of cellular bioenergetics. Current approaches for study...

Glutamine is the most abundant amino acid in serum, used as a key nutrient by cells for protein synthesis, energy production, carbon and nitrogen metabolism, and cellular redox balance. The use of glutamine in the cell is highly compartmentalized, but the dynamics of glutamine metabolism across organelles and individual cells are not fully understood. To illuminate subcellular glutamine dynamics, we developed an intracellular glutamine optical reporter, iGlo. We find iGlo is sensitive and specific for glutamine and can be used to measure glutamine uptake, production, and consumption with high spatiotemporal resolution in multiple cell types. Furthermore, multiplexed imaging of iGlo with a lactate biosensor in single cells reveals temporal crosstalk between glucose and glutamine metabolism to maintain energy homeostasis. Thus, iGlo enables the sensitive and precise study of compartmentalized glutamine dynamics and represents a new and enhanced tool for studying the spatiotemporal dynamics and regulation of metabolism. ### Competing Interest Statement The authors have declared no competing interest. National Institutes of Health, https://ror.org/01cwqze88, T32GM145388, P30CA016042, R35GM138003, DP2GM154012 United States Department of Energy, https://ror.org/01bj3aw27, DE-FC02-02ER63421 UCLA Jonsson Comprehensive Cancer Center, https://ror.org/0599cs764, Strategic Plan Aligned Grant, Graduate Research Award Chan Zuckerberg Initiative (United States), https://ror.org/02qenvm24, MET-000000000151

Phosphatase and tensin homologue-induced kinase 1 (PINK1) is a serine/threonine kinase that plays roles in mitophagy, cell death, and regulation of cellular bioenergetics. Current approaches for study...

President Trump's higher education battle comes to the University of California. After the Department of Justice found that UCLA had violated Jewish students' civil rights, the administration froze hu...

This year’s federal funding cuts are severely impacting STEM research and education, threatening the future of innovation and our nation’s health and technological advancement. These cuts hav…

The Trump administration cited alleged antisemitism at UCLA as a reason for suspending the funding. Years worth of research could be lost, one UCLA researcher said.

Malonyl-CoA is the essential building block of fatty acids and regulates cell function through protein malonylation and allosteric regulation of signaling networks. Accordingly, the production and use...

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