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Keith Zientek

@zientek.bsky.social
59 followers 44 following 73 posts

Core scientist

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Keith Zientek @zientek.bsky.social · 12h
If they can put the Doom video game on a TI-83 why can't we analyze data on a graphing calculator?
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Keith Zientek @zientek.bsky.social · 01/10/2026
What's the impact factor of your photo library?
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Keith Zientek @zientek.bsky.social · 25/09/2026
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Keith Zientek @zientek.bsky.social · 10/09/2026
Once a week get takeout from every place serving food within a 20-30 min walk round trip.
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Keith Zientek @zientek.bsky.social · 28/08/2026
Maybe it will be an “x formerly known as Twitter” situation where all the words are used so the keywords still apply.
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Keith Zientek @zientek.bsky.social · 20/08/2026
Read Old Man's when it came out. If you like those you might want to check out the Bobiverse series by Dennis Taylor or the Culture books by Iain Banks.
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Keith Zientek @zientek.bsky.social · 20/08/2026
I ran out of characters. I did enjoy the interdependency series by Scalzi. That was some good old school sci-fi. I have a hold on Ancillary Justice at the library right now.
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Keith Zientek @zientek.bsky.social · 20/08/2026
In the last few years I have enjoyed the writings of: NK Jemison(Broken Earth Series) Joe Abercrombie(Age of Madness & Shattered Sea series) Christopher Buehlman(Blacktongue Thief and Between Two Fires) Adrian Tchaikovsky(Service Model) Martha Wells(Muderbot series) Paolo Bacigalupi(Water Knife)
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Keith Zientek @zientek.bsky.social · 20/08/2026
I have a bunch of sci-fi fantasy suggestions when you're ready.
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Keith Zientek @zientek.bsky.social · 19/08/2026
Le Guin was a local for me. Once my mom went to Audubon society craft fair in Portland and she was just chilling at a card table signing a children's book she wrote about cats having dreams.
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Keith Zientek @zientek.bsky.social · 30/07/2026
Do you know where they got those shirts?
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Keith Zientek @zientek.bsky.social · 26/07/2026
If you ever make your way to Portland, we have a pretty good pinball museum. www.nextlevelpinballmuseum.com
nextlevelpinballmuseum.com
620+ games on Free Play | Next Level Pinball Museum | Hillsboro, Oregon
Next Level Pinball Museum is one of the world's largest arcades with over 620+ games on free play. We are a mixture of pinball machines and classic and modern arcade games as well as a 27,000 square f...
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Keith Zientek @zientek.bsky.social · 23/07/2026
What is everyone using for only albumin depletion with #proteomics nowadays? We had been using an Agilent spin column but it has been discontinued.
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Keith Zientek @zientek.bsky.social · 21/07/2026
We've been using them for years now and the variability is not a problem.
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Keith Zientek @zientek.bsky.social · 13/07/2026
The only consistent thing throughout history is that we are doomed to repeat it.
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Reposted by Keith Zientek
Sarah Gollust @sarahgollust.bsky.social · 11/07/2026
Did you write your comment yet on the OMB rules on federally funded science yet? No? Don’t worry, you can do it this weekend. Here are some resources that can help you get started.
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Keith Zientek @zientek.bsky.social · 25/05/2026
xkcd.com/435/
xkcd.com
Purity
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Reposted by Keith Zientek
Lee Cantrell @leecantrell.bsky.social · 18/05/2026
www.biorxiv.org/content/10.6... 1/ Proteomics method development often optimizes for IDs. But for cohort biology, IDs only matter if they remain quantitative, precise, and powered. We ask how chromatographic sampling density propagates from peaks → LOQ → discovery. 🧵
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Keith Zientek @zientek.bsky.social · 01/05/2026
One of the common booths you would see at the cons would be people selling bootleg VHS tapes and cassettes for $5-10. So you would find all sorts of stuff from fansubbed anime to the musical episode of Buffy to old episodes of Red Dwarf.
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Keith Zientek @zientek.bsky.social · 01/05/2026
I remember going to a sci fi convention in the early 90s and someone was selling copies of that song on a cassette tape for $5.
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Keith Zientek @zientek.bsky.social · 08/04/2026
Jealous of those gas prices. I'm seeing $4.70-$5.00 locally.
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Keith Zientek @zientek.bsky.social · 16/03/2026
They could get a lot of work done if it wasn't for all those pesky students at their university designed for educating students.
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Keith Zientek @zientek.bsky.social · 05/03/2026
Oh dang infinite resolution! That's got to be a fun duty cycle.
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Keith Zientek @zientek.bsky.social · 28/02/2026
Think about all the extra papers we can write if we don’t need to show our work!
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Keith Zientek @zientek.bsky.social · 13/02/2026
Probably someone named Ben.
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Reposted by Keith Zientek
Phil W @pwilmarth.bsky.social · 29/01/2026
If you are interested in how modern proteomics can shed light on rare childhood diseases, check out this new paper: www.frontiersin.org/journals/mol... TMTpro quantitation works really well. Heads up: it is not a short story. There are decades of knowledge behind the interpretation of the results.
frontiersin.org
Frontiers | The Nodding syndrome cerebrospinal fluid proteome: a lens into neurodevelopmental failure consistent with environmentally triggered MECP2 dysregulation?
IntroductionNodding Syndrome (NS) is a childhood-onset epileptic encephalopathy of unknown etiology, occurring in clustered outbreaks across East Africa. Des...
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Keith Zientek @zientek.bsky.social · 06/01/2026
There's so many more peptides to discover in the same HELA digest!
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Keith Zientek @zientek.bsky.social · 02/12/2025
AI will never be smart enough to figure out the true mysteries of science like why that one tube dries slower than the others in the SpeedVac.
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Reposted by Keith Zientek
Takumi Tsutaya @tsutatsuta.bsky.social · 20/11/2025
The Python script reported in Mackie et al. 2018 (Angew Chem Int 57:736–7374) for calculating N and Q deamidation rates from proteomic results has now been ported to the R environment. GitHub github.com/tsutatsuta/r... Zenodo doi.org/10.5281/zeno...
github.com
GitHub - tsutatsuta/rdeamidation: Calculates deamidation rates of N and Q for proteomic results
Calculates deamidation rates of N and Q for proteomic results - tsutatsuta/rdeamidation
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Keith Zientek @zientek.bsky.social · 03/09/2025
At some point all the AI bots will just talk to each other via Office and Teams and we can really get some work done while they are distracted.
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Keith Zientek @zientek.bsky.social · 29/08/2025
It really forces you to look at the abstract qualities of your skills and experience. From the exciting stuff like troubleshooting and instrument operation to the common skills like excel spreadsheets, etc.
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Keith Zientek @zientek.bsky.social · 29/08/2025
I went through this 6 years ago. Realistically there were only two places in my city that were a match for my skillset. I worked for one and they were shut down. While I was trying to get a job at #2, I went on a bunch of interviews and applied for jobs that were overlapping with my background.
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Keith Zientek @zientek.bsky.social · 27/08/2025
I'm not familiar with the plus but I have used the QE for a PRM method with an inclusion list. It's a weirdly worded setting. You can either have it cycle through the inclusion list or have it trigger DDA scans if the masses on the list aren't detected.
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Keith Zientek @zientek.bsky.social · 14/07/2025
I guess you could get a job at a company doing something similar but you would have to add one bajillion zoom calls that could have been emails to your weekly schedule.
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Keith Zientek @zientek.bsky.social · 20/06/2025
Not proteomics but I am reminded of the petroleum work done by Alan Marshall’s lab
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Keith Zientek @zientek.bsky.social · 19/06/2025
Now I want to know about variants 1 through 6.
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Keith Zientek @zientek.bsky.social · 12/06/2025
When I was a first year grad student I had the opportunity to meet him. He was incredibly kind to me and an influential advocate for the power of high resolution mass spectrometry. He will be missed.
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Keith Zientek @zientek.bsky.social · 02/06/2025
I hope it can answer advanced PhD level queries like where is the cheapest cafeteria and where is the nice bathroom.
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Reposted by Keith Zientek
Randall Munroe @xkcd.com · 29/05/2025
Mass Spec xkcd.com/3094/
[Person with ponytail wearing lab coat hands balloon to another person in front of a springboard, a magnet hanging from above, and then a target] PERSON WITH PONYTAIL to Person 2: Rub this balloon against your head, then go jump past that magnet toward the target on the wall. [caption] Before the bathroom scale was invented, the only way to weigh people was mass spectrometry.
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Keith Zientek @zientek.bsky.social · 15/05/2025
Use the free time to run more Western blots as required by reviewer 3 for every paper.
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Keith Zientek @zientek.bsky.social · 28/04/2025
Quick invest in an autosampler vial company.
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Keith Zientek @zientek.bsky.social · 20/03/2025
Why limit yourself to just the hot dogs when you can work in the cafe and operate the pizza robot? youtu.be/c-E2Cz59_5A?...
youtu.be
🍕 Pizza making at Costco Too Quick to eat Pizza robot unmanned pizza 🍕 😋
YouTube video by Hariax Thoria
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Keith Zientek @zientek.bsky.social · 20/03/2025
Imagine the savings if you had an employee discount on top of the normal deals. And easy access to the hot dogs!
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Keith Zientek @zientek.bsky.social · 20/03/2025
Was hired to run a very small bioanalytical core and learned on the job with help from biochemistry post docs and a neighboring proteomics mass spec core facility.
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Keith Zientek @zientek.bsky.social · 18/03/2025
When identifying candidates form untargeted quan for further study/grants do you ever go back and look at the raw data to see things like # of peptides per protein and # of points per peak?
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Keith Zientek @zientek.bsky.social · 18/03/2025
Is that >2 peptides per protein?
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Keith Zientek @zientek.bsky.social · 06/03/2025
I’d love to see people consider metrics other than # of proteins detected.
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Keith Zientek @zientek.bsky.social · 05/03/2025
I bet you could get a bunch of Zip Drive discs or 3.5 floppys at a deep discount.
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Keith Zientek @zientek.bsky.social · 12/02/2025
Nevertheless Science For Work
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Keith Zientek @zientek.bsky.social · 10/01/2025
I wonder how many Terrabytes or Petabytes of Astral raw files would be generated in that study? Maybe even get to Exabytes?
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