Keith Zientek @zientek.bsky.social · 23/07/2026What is everyone using for only albumin depletion with #proteomics nowadays? We had been using an Agilent spin column but it has been discontinued. 001
Reposted by Keith ZientekSarah Gollust @sarahgollust.bsky.social · 11/07/2026Did you write your comment yet on the OMB rules on federally funded science yet? No? Don’t worry, you can do it this weekend. Here are some resources that can help you get started. 10414326
Reposted by Keith ZientekLee Cantrell @leecantrell.bsky.social · 18/05/2026www.biorxiv.org/content/10.6... 1/ Proteomics method development often optimizes for IDs. But for cohort biology, IDs only matter if they remain quantitative, precise, and powered. We ask how chromatographic sampling density propagates from peaks → LOQ → discovery. 🧵 1204
Reposted by Keith ZientekPhil W @pwilmarth.bsky.social · 29/01/2026If you are interested in how modern proteomics can shed light on rare childhood diseases, check out this new paper: www.frontiersin.org/journals/mol... TMTpro quantitation works really well. Heads up: it is not a short story. There are decades of knowledge behind the interpretation of the results.frontiersin.orgFrontiers | The Nodding syndrome cerebrospinal fluid proteome: a lens into neurodevelopmental failure consistent with environmentally triggered MECP2 dysregulation?IntroductionNodding Syndrome (NS) is a childhood-onset epileptic encephalopathy of unknown etiology, occurring in clustered outbreaks across East Africa. Des... 011
Reposted by Keith ZientekTakumi Tsutaya @tsutatsuta.bsky.social · 20/11/2025The Python script reported in Mackie et al. 2018 (Angew Chem Int 57:736–7374) for calculating N and Q deamidation rates from proteomic results has now been ported to the R environment. GitHub github.com/tsutatsuta/r... Zenodo doi.org/10.5281/zeno...github.comGitHub - tsutatsuta/rdeamidation: Calculates deamidation rates of N and Q for proteomic resultsCalculates deamidation rates of N and Q for proteomic results - tsutatsuta/rdeamidation 164
Keith Zientek @zientek.bsky.social · 12/06/2025When I was a first year grad student I had the opportunity to meet him. He was incredibly kind to me and an influential advocate for the power of high resolution mass spectrometry. He will be missed. 010
Keith Zientek @zientek.bsky.social · 22/02/2024USHUPO tips. Flying into Portland and staying at the conference hotel? The light rail goes from the airport to a stop right by the hotel. The line is closed for construction currently but is scheduled to be up and running by March 3rd. trimet.org/traintoplane/ 000
Keith Zientek @zientek.bsky.social · 22/02/2024As inspiration strikes I will be posting tips for attendees of USHUPO in Portland. #proteomics 010
Keith Zientek @zientek.bsky.social · 22/02/2024USHUPO tips. Vegan attendees will find lots of options. Portland is very vegan friendly. www.pdxmonthly.com/eat-and-drin... 000
Keith Zientek @zientek.bsky.social · 22/02/2024USHUPO tips. The fentanyl crisis is hitting Portland hard. Property crime is high in certain areas. Don’t leave items unattended in rental cars. Old Town is particularly rough right now. 000
Keith Zientek @zientek.bsky.social · 22/02/2024USHUPO tips. There are many amazing neighborhoods and areas aside from downtown. I would suggest the following: NE Alberta Arts District SE Hawthorne SE Division Goose Hollow NE Mississippi Ave travelportland.com has good descriptions of these areas. 000
Keith Zientek @zientek.bsky.social · 22/02/2024USHUPO tips. Portland is well known for beer. There are many small breweries that don’t distribute out of the region preferring to focus on local customers. This blogger is the most influential Portland beer writer IMO. www.beervanablog.com/beervana/202...beervanablog.comPortland's Best Breweries — BeervanaPortland is inarguably one of the country’s great beer cities, but which breweries are the jewels in Beervana’s crown? In my annual feature, I offer the ten breweries doing the best work right now, ... 040
Keith Zientek @zientek.bsky.social · 22/02/2024USHUPO tips. Eater PDX has decent neighborhood guides with maps. This one is for the area around the hotel. pdx.eater.com/maps/restaur... 000
Keith Zientek @zientek.bsky.social · 22/02/2024USHUPO tips. Voodoo donuts is mostly visited by tourists. Locals in that area tend to go to Delicious Donuts, Mikikio Mochi Donuts or Doe Donuts. 030
Keith Zientek @zientek.bsky.social · 04/11/2023Would there be interest in a local written guide to the area for HUPO in Portland? #proteomics 262
Keith Zientek @zientek.bsky.social · 05/10/2023Good blog post about how to approach the analysis of a two species proteomics experiment. github.com/pwilmart/Hum... 082
![[Person with ponytail wearing lab coat hands balloon to another person in front of a springboard, a magnet hanging from above, and then a target] PERSON WITH PONYTAIL to Person 2: Rub this balloon against your head, then go jump past that magnet toward the target on the wall. [caption] Before the bathroom scale was invented, the only way to weigh people was mass spectrometry.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:cz73r7iyiqn26upot4jtjdhk/bafkreid7lnvd7afmasx3nxi6anxmyhbatpaw54za3kiw3eaoclrf722faq)