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Vojtech Dostal

@vojtechdostal.bsky.social

122 followers 261 following 27 posts

Cell biologist at @ibtcas.bsky.social. Wikipedia & Wikidata guy.

Vojtech Dostal @vojtechdostal.bsky.social · 01/08/2026

BORC antibodies are generally very bad on IF, but this one turned out quite nice yesterday ... and thus we can validate one more peripheral lysosomal protein!

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Vojtech Dostal @vojtechdostal.bsky.social · 23/07/2026

Another attempt to demonstrate my two lysosomal subpopulations, this time live with overexpressed EPDR1 and LAMP2B.

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Vojtech Dostal @vojtechdostal.bsky.social · 17/06/2026

My project at @i-med.ac.at focused on lysosomal proteins which show an uneven distribution between perinuclear and peripheral pools (www.biorxiv.org/content/10.6...) Here's one identified perinuclear lysosomal protein, EPDR1-mCherry (magenta), expressed in U2OS cells alongside LAMP1 (green).

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Vojtech Dostal @vojtechdostal.bsky.social · 27/02/2025

An old timelapse I dug up... U937-derived macrophages bathed in green and red dextran... the green dextran is pH sensitive, leading to the appearance of mostly red lysosomes.

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Vojtech Dostal @vojtechdostal.bsky.social · 16/02/2025

CellPose 3

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Vojtech Dostal @vojtechdostal.bsky.social · 16/02/2025

MicroSam

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Vojtech Dostal @vojtechdostal.bsky.social · 16/02/2025

SamCell results

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Vojtech Dostal @vojtechdostal.bsky.social · 16/02/2025

For testing, I used these three DIC images (which I so far never segmented automatically and had to rely on manual ROI selection) - a tissue culture room microscope, an Incucyte image and a high-end confocal DIC channel

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Vojtech Dostal @vojtechdostal.bsky.social · 07/02/2025

Very nice! During our practical course we once used the ColorfulCell vector for a similar purpose www.addgene.org/62449/

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Vojtech Dostal @vojtechdostal.bsky.social · 04/12/2024

ER-lysosome contact sites (green) in MEFs on the background of an ER stain (grey, GFP-VAPA)

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