Reposted by Vincent Spreng
1/28 How do you optimize a dynamic protein property that emerges from multiple states? Our finally published paper in @NatureComms takes on one of the hardest problems in protein engineering with phage assisted evolution: evolving allosteric switches🧵
![A. Structure of AsLOV2 [PDB 2V1A] with mutations resutling in MagLOV~2 highlighted. For the first time, quantum sensing in living cells using a fluorescent protein-based sensor can be performed by driving spin transitions using radio-frequency (RF) fields in the presence of a static magnetic field. B. Simplified photocycle diagram in the case of a large external magnetic field.
C. Microscope image cropped to a single cell expressing MagLOV 2. Scalebar is 1 um.
D. For MFE measurements the magnetic field was modulated between 0 mT and 10 mT, here with a period 10 seconds. The intensity over time is integrated over pixels covering the single cell, with an exponential decay (due to photobleaching) removed.
E. A single cell expressing MagLOV 2 displaying an ODMR signal 4% contrast.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:tludcv74nxumusfwzkmfcl5q/bafkreigmpmmy3xz6alxsxwwmsw4dxn7lkebjl4lqtcwvpiljdiubztlmqa)