Sign in

Stefano Donati

@stefanodonati.bsky.social
504 followers 98 following 24 posts

Understanding and engineering microbial metabolism DTU BRIGHT, formerly DTU Biosustain. www.biosustain.dtu.dk/research/rese…

PostsRepliesMedia
Stefano Donati @stefanodonati.bsky.social · 08/06/2026
Happy to share our brand-new toolset for growth data analysis! This includes a python package and two apps for the analysis of data from plate readers (MicroGrowth) and mini-bioreactors (AutoGrowth). Everything is free and open-source. Link to the apps: biosustain.github.io/growthcurves...
biosustain.github.io
Growth Curves App
133
Reposted by Stefano Donati
Giovanni Schiesaro @giovanschi.bsky.social · 08/06/2026
Studying fungal organisms can be challenging, and we need more versatile tools or protocols that could be easily transferred from one fungal organism to another.
121
Stefano Donati @stefanodonati.bsky.social · 09/03/2026
New preprint from our group! When we first started working with affordable, continuous-culture mini-bioreactors a few years ago, we realized they offered a unique solution for CRISPRi experiments, which rely on cell growth to dilute targeted proteins. www.biorxiv.org/content/10.6...
biorxiv.org
122
Stefano Donati @stefanodonati.bsky.social · 12/08/2025
1/ 🚨 New preprint! We've developed a robust IDMS-based LC-MS/MS workflow for high-throughput intracellular metabolomics in diverse bacteria. www.biorxiv.org/content/10.1... #Metabolomics #MassSpec #SystemsBiology
biorxiv.org
A Robust Isotope Ratio LC-MS/MS Workflow for High-Throughput Metabolic Profiling of Bacteria
Stable isotope dilution mass spectrometry (IDMS) has become a cornerstone of quantitative metabolomics, enabling accurate intracellular metabolite quantification across a range of biological systems. ...
143
Stefano Donati @stefanodonati.bsky.social · 26/07/2025
Now published in Microbial Biotechnology! enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10....
enviromicro-journals.onlinelibrary.wiley.com
052
Stefano Donati @stefanodonati.bsky.social · 15/06/2025
Heading over to #ME16, excited to catch up with many old friends and collaborators! If you are there, make sure to check out posters from our group: @magnusgj.bsky.social poster nr 203 sunday/monday; Mariana Arango Saavedra and Catarina Rocha posters 52 and 53 on monday/tuesday
130
Stefano Donati @stefanodonati.bsky.social · 25/04/2025
Hot off the press! 📰 Excited to share our latest opinion piece, co-authored with Christopher Johnson, where we explore why Cupriavidus necator stands out as a top microbial host for converting C1 substrates into valuable products www.sciencedirect.com/science/arti...
sciencedirect.com
Optimizing Cupriavidus necator H16 as a host for aerobic C1 conversion
Biological systems capable of converting CO2 or CO2-derived, single-carbon (C1) compounds can be used to reduce or reverse carbon emissions while esta…
060
Stefano Donati @stefanodonati.bsky.social · 27/03/2025
📢 Preprint alert! In this study we provide new genomic insights into #CupriavidusNecator for #C1Biomanufacturing: - Genome assemblies for more strains - Species definition refined - Traits & methylation systems explored Great job by @magnusgj.bsky.social and team www.biorxiv.org/content/10.1...
biorxiv.org
Comparative genomic assessment of the Cupriavidus necator species for one-carbon based biomanufacturing
The transition from a petroleum-based manufacturing to biomanufacturing is an important step towards a sustainable bio-economy. In particular biotechnological processes which use one carbon (C1) compo...
132
Reposted by Stefano Donati
Pavel Dvořák @pavelcito.bsky.social · 20/02/2025
Good news everyone! (I'd need more good news these days) I'm happy to share here our latest contribution in COBIOT on advances in engineering substrate scope in Pseudomonas cell factories. Great collaboration with @stefanodonati.bsky.social and Miguel Silva! www.sciencedirect.com/science/arti...
sciencedirect.com
Advances in engineering substrate scope of Pseudomonas cell factories
Most current industrial bioprocesses use well-established model microorganisms and simple sugar substrates from edible starch or molasses. To broaden …
084
Stefano Donati @stefanodonati.bsky.social · 18/12/2024
📣 Exciting news! The Novo Nordisk Foundation will continue to support an excellence center for #biotechnology at #DTU, building on the legacy of DTU Biosustain over the past 14 years. Grateful to have been part of this! Now an even BRIGHTer future ahead 🌟 novonordiskfonden.dk/en/news/new-...
novonordiskfonden.dk
New initiative aims to strengthen the bio-based economy - Novo Nordisk Fonden
030
Stefano Donati @stefanodonati.bsky.social · 11/12/2024
Proud of the efforts of our team to deeply think about how to improve practices our laboratory, now certified LEAF bronze (hopefully soon silver and gold!). Thank you especially to Baris Kara who led the effort as well as Mariana Arango Saavedra and Catarina Rocha
051
Reposted by Stefano Donati
Sheila I. Jensen @sheilaijensen.bsky.social · 13/11/2024
We recently published an article, where we through genetic engineering and CRISPRi enable synthetic anaerobic and aerobic physiology in one bioreactor. Two fermentations in one go. Give it a read. www.nature.com/articles/s41...
nature.com
CRISPRi-mediated metabolic switch enables concurrent aerobic and synthetic anaerobic fermentations in engineered consortium - Nature Communications
An important parameter for synthetic multi-strain bioprocess development is population control and stability. Here, the authors developed a synthetic consortium platform consisting of two engineered E...
0164
Stefano Donati @stefanodonati.bsky.social · 19/11/2024
Judging on the number of followers I got without posting almost anything 😅 I assume a large part of the scientific community is actually moving here! Genuinely curious whether this science-twitter 2.0 will work out
130
Stefano Donati @stefanodonati.bsky.social · 21/10/2023
In our most recent perspective, we argue that we need to bridge more effectively knowledge on C1-trophy obtained in synthetic and natural organisms, and that this approach will enable the engineering of efficient microbes for C1-based biomanufacturing www.nature.com/articles/s41...
nature.com
Synergistic investigation of natural and synthetic C1-trophic microorganisms to foster a circular ca...
Using one carbon (C1) molecules as primary feedstock for bioproduction holds great potential for a circular and carbon neutral economy. Here, the authors discuss the potential of merging knowledge gai...
131
Stefano Donati @stefanodonati.bsky.social · 11/10/2023
We are recruiting a Postdoc and a Research Assistant to work with us on systems biology C1-trophic microorganisms @DTUBiosustain. Last week to apply! For more info and to apply: - Postdoc shorturl.at/wFNR8 - RA shorturl.at/dJKU1
000
Stefano Donati @stefanodonati.bsky.social · 04/10/2023
Finally made it to the "new place".. really curious to see whether the scientific community will actually move here
031