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Stefano Giandomenico

@sgiando.bsky.social

Undergrad @OfficialUoM | PhD @MRC_LMB, Cambridge | Postdoc @mpi_brain, Frankfurt am Main | Neuroscience, organoids & synapse enthusiast 🧠

Posts Replies Media

Stefano Giandomenico @sgiando.bsky.social · 30/09/2026

This was a shared effort with @marcvoo.bsky.social while in the lab of @erin-schuman.bsky.social

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Stefano Giandomenico @sgiando.bsky.social · 30/09/2026

Correlation analysis of synaptosomes from our organoids and those from mouse cortex, prepped in the same way, shows that neural organoids recapitulate in vivo synaptic proteome enrichment patterns. 5/5

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Stefano Giandomenico @sgiando.bsky.social · 30/09/2026

To systematically characterize the protein composition of organoid synapses, we fluorescently labeled and purified synaptosomes by fluorescence-activated synaptosome sorting (FASS) before mass spectrometry. 4/5

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Stefano Giandomenico @sgiando.bsky.social · 30/09/2026

We performed proteomics across the differentiation protocol (>7,000 detected proteins per stage). Synaptic proteins ramp up sharply after day 11. 3/5

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Stefano Giandomenico @sgiando.bsky.social · 30/09/2026

We differentiated mESCs into mature forebrain organoids. They express excitatory and inhibitory synapse markers (Vglut1, Vgat, Psd95, Gephyrin) and show spontaneous calcium activity that TTX blocks, indicating action-potential-dependent network activity. 2/5

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Stefano Giandomenico @sgiando.bsky.social · 30/09/2026

Can a brain organoid grown in a dish build synapses that resemble the real thing at the molecular level? 🧠 We grew mouse forebrain organoids for 35 days and compared their synaptic proteome to that of the adult mouse cortex 🧪 www.biorxiv.org/content/10.6... 🧵 1/5

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Reposted by Stefano Giandomenico

Mara Mueller @muellermara.bsky.social · 28/04/2025

New Preprint out ! The proteasome maturation factor POMP moonlights as a stress-induced transcriptional regulator www.biorxiv.org/content/10.1... Lead by: @sgiando.bsky.social with: @muellermara.bsky.social , @erin-schuman.bsky.social , @marcvoo.bsky.social and many more Short thread below 🧵1/4

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