iiinova — where your digital self keeps evolving

Reposted by Sarah Gersing

Kresten Lindorff-Larsen @lindorfflarsen.bsky.social · 18/01/2026

Second preprint of the year in which @sarahgersing.bsky.social from @rhp-lab.bsky.social mapped the effects of >7500 variants in glucokinase (GCK) on the interaction with the glucokinase regulatory protein so that we now have a 3D GCK scan (abundance, interaction, activity) doi.org/10.64898/202...

Figure 2 from the paper with: (A) Heatmap showing the interaction score of each possible amino acid substitution along the GCK sequence. (B) Interaction score distributions of missense, synonymous and nonsense variants. (C) The median interaction score of each GCK residue mapped onto the structure of the GCK–GKRP complex.

0236

Reposted by Sarah Gersing

Rasmus Hartmann-Petersen @rhp-lab.bsky.social · 16/01/2026

New preprint! Led by @sarahgersing.bsky.social we map how 7,500+ variants in glucokinase (GCK) affect binding to GKRP and disentangle this from stability. We now have activity, abundance, and interaction scores for 7,128 GCK variants - a resource for understanding phenotypes and glucose homeostasis.

0166

Reposted by Sarah Gersing

Rasmus Hartmann-Petersen @rhp-lab.bsky.social · 18/05/2025

With the @lindorfflarsen.bsky.social group we present our map of degrons in all human transcription factors, incl. examples of constitutive degrons in exposed regions & buried degrons that are exposed upon mutation. In addition, we show that most TADs overlap with degrons. Work led by Fia Larsen.

biorxiv.org

Comprehensive degron mapping in human transcription factors

Gene expression is regulated by the targeted degradation of transcription factors through the ubiquitin-proteasome system. Transcription factors destined for degradation are recognized by E3 ubiquitin...

0297

Reposted by Sarah Gersing

Rasmus Hartmann-Petersen @rhp-lab.bsky.social · 15/05/2025

In collaboration with the @lindorfflarsen.bsky.social group we release our map of degrons in >5,000 human cytosolic proteins with >99% coverage. A machine learning model trained on the data identifies missense variants forming degrons in exposed & disordered regions. Work led by @vvouts.bsky.social.

biorxiv.org

A complete map of human cytosolic degrons and their relevance for disease

Degrons are short protein segments that target proteins for degradation via the ubiquitin-proteasome system and thus ensure timely removal of signaling proteins and clearance of misfolded proteins fro...

0188

Reposted by Sarah Gersing

Kresten Lindorff-Larsen @lindorfflarsen.bsky.social · 06/03/2025

Do you think that cooperativity requires more than one binding site or subunit? Or just want to read about an interesting enzyme for which both gain- and loss-of-function variants can cause metabolic disease? Then check out @sarahgersing.bsky.social's review on glucokinase: doi.org/10.1016/j.ti...

0134

Reposted by Sarah Gersing

Trends in Biochemical Sciences @cp-trendsbiochem.bsky.social · 03/01/2025

Now online - the Review "#Glucokinase: from allosteric glucose sensing to disease variants" from @sarahgersing.bsky.social, Torben Hansen, @lindorfflarsen.bsky.social, and @rhp-lab.bsky.social. #GlucoseHomeostasis #GlucoseSensor #GCKMODY #CooperativeKinetics authors.elsevier.com/a/1kN5M3S6Gf...

183

Sarah Gersing @sarahgersing.bsky.social · 02/01/2025

Happy to share our review, 'Glucokinase: from allosteric glucose sensing to disease variants', published in @cp-trendsbiochem.bsky.social, which explores the fascinating enzyme glucokinase. authors.elsevier.com/a/1kN5M3S6Gf...

authors.elsevier.com

1192