Reposted by Rubaba
New article from the lab on HaloTag-based chemical proteomics in RSC Chemical Biology. Simple, fast and highly selective labelling of probe-protein conjugates allow gel-based shift assay and pull-downs followed by MS proteomics. Congratulations @rubaba.bsky.social! pubs.rsc.org/en/content/a...
pubs.rsc.org
A cysteine reactive chloroalkane probe enables HaloTag ligation for downstream chemical proteomics analysis
Chemical proteomics is a powerful method to track proteins labelled by reactive small molecules in living cells on a proteome-wide scale. The strategy relies on the reactivity and specificity of bioor...
