iiinova — where your digital self keeps evolving

Reposted by Rosalie Lawrence

asamelson @asamelson.bsky.social · 28/01/2026

After a long review process, I'm excited that our paper is finally in print: www.cell.com/cell/fulltex... TL;DR: We use CRISPR screens in iPSC-derived neurons to find a new tau E3 ligase and a relationship between oxidative stress, the proteasome, and tau proteolytic fragments. More below 👇

cell.com

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis

CRISPR screens in iPSC-derived neurons reveal that the E3 ubiquitin ligase CRL5SOCS4 ubiquitinates tau, that CUL5 expression is correlated with resilience in human Alzheimer’s disease, and that electr...

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Reposted by Rosalie Lawrence

Willow Coyote-Maestas @willowcoyote.bsky.social · 31/10/2025

Zara Weinberg passed away earlier this week. She joined my lab earlier this year when her postdoc lab formally closed. She was a brilliant scientist, supportive mentor, dear friend, avid music fan, rabid believer in public transit, open science champion, and above all just an amazing human.

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Reposted by Rosalie Lawrence

Jay Brito Querido @jbquerido.bsky.social · 22/07/2025

Finally out! I’m thrilled to share our new paper (Wolin et al., Cell 2025). This paper describes SPIDR, a high-throughput method for mapping RBP binding sites. By combining #SPIDR with #cryoEM, we identified the exact binding site of LARP1 within the #mRNA channel of the 40S ribosomal subunit.

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Rosalie Lawrence @rosalielawrence.bsky.social · 22/07/2025

Thanks Brooke! At UCLA students first need to be admitted into one of our graduate programs, so I can’t directly recruit grad students.

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Rosalie Lawrence @rosalielawrence.bsky.social · 22/07/2025

Hi Brooke, can I be added to your starter pack? Just opened a lab on mechanisms of stress response signaling (Lawrence-lab.org)

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Rosalie Lawrence @rosalielawrence.bsky.social · 21/07/2025

Hi Moe, can I be added to your starter pack? Just opened a lab on mechanisms of stress response signaling and metabolic regulation (Lawrence-lab.org)

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Rosalie Lawrence @rosalielawrence.bsky.social · 04/07/2025

Hey that protein looks familiar! 🦋

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Reposted by Rosalie Lawrence

Alexey Amunts @amunts.bsky.social · 04/07/2025

Speak up to prevent a major loss for the field. CASP, which helped shaping structural biology including AlphaFold is on the verge of shutting down. NIH funding has lapsed, UC Davis support ends and the core team is being let go. Painfully shortsighted.

science.org

Exclusive: Famed protein structure competition nears end as NIH grant money runs out

Agency silent on funding renewal for contest that inspired creation of AIs that predicted how proteins would fold

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Rosalie Lawrence @rosalielawrence.bsky.social · 16/02/2025

So excited to co-chair this session with @herrflow.bsky.social tomorrow! Come hear cool talks ranging from computational to experimental approaches to understand protein dynamics and allostery!

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Reposted by Rosalie Lawrence

Theanne Griffith, PhD 🇧🇧👩🏽‍🔬🇭🇷 @doctheagrif.bsky.social · 15/02/2025

Attending #BPS2025? Want to know more about tangible steps you can take to challenge the attacks on science in the U.S.? Please attend an Emergency Town Hall Meeting on Tuesday at 1:30! @biophysicalsoc.bsky.social @blackinbiophys.bsky.social Please spread the word!

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Rosalie Lawrence @rosalielawrence.bsky.social · 28/01/2025

Great news, great opportunity for students and postdocs!!

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Rosalie Lawrence @rosalielawrence.bsky.social · 10/12/2024

Hi Sarah, can you add me? Thanks for making this list!

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Rosalie Lawrence @rosalielawrence.bsky.social · 20/11/2024

Thanks for making this! Can you add me?

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Reposted by Rosalie Lawrence

Zhicheng Cui | 崔志成 @chen3dem.bsky.social · 17/11/2024

First post on bluesky gotta be the structure of mTORC1 on membrane! A great collaboration and interesting frontier for SPA cryoEM!

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Reposted by Rosalie Lawrence

Itai Yanai @itaiyanai.bsky.social · 18/11/2024

If you don’t feel stupid doing science, you’re not trying hard enough.

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Reposted by Rosalie Lawrence

Elisa Zhang, Ph.D. @elisazhang.bsky.social · 13/11/2024

Our lab is hiring a postdoc! We have multiple projects in uterine, placental, and pregnancy biology. Candidates with a background in stem cells, development, reproduction, or bioinformatics are welcome to apply. See the attached ad and visit zhang.faculty.ucdavis.edu for more info.

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Rosalie Lawrence @rosalielawrence.bsky.social · 13/11/2024

Can you add me please? Opening a metabolism and stress response lab at UCLA in January!

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Rosalie Lawrence @rosalielawrence.bsky.social · 13/11/2024

Technician positions, ideal of recent BA grads interested in gaining more experience before grad school: SRA I Cell biology: www.uclahealthcareers.org/job/21223665... SRA I Biochemistry: www.uclahealthcareers.org/job/21223666...

uclahealthcareers.org

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Rosalie Lawrence @rosalielawrence.bsky.social · 13/11/2024

The Lawrence Lab website (lawrence-lab.org) is live and we are hiring for our launch in Jan 2025! We combine high resolution structure 🔬 and cell biology 🧪🧫 approaches to define mechanisms of stress response signaling in health and disease. Live postings below--Please RT and send to recent grads!

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Reposted by Rosalie Lawrence

asamelson @asamelson.bsky.social · 28/10/2024

Our updated preprint is live! www.biorxiv.org/content/10.1... @kampmann.bsky.social We add new data: 1. A new CRL5 complex that targets tau for degradation 2. CUL5 as a marker of vulnerability in Alzheimer's Disease 3. Oxidative stress-derived tau fragments change in vitro tau aggregation

biorxiv.org

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis

Aggregation of the protein tau defines tauopathies, which include Alzheimer's disease and frontotemporal dementia. Specific neuronal subtypes are selectively vulnerable to tau aggregation and subseque...

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Rosalie Lawrence @rosalielawrence.bsky.social · 23/10/2024

Thanks @tlnagy.bsky.social ! I'm excited to join the UCLA community 😊

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Rosalie Lawrence @rosalielawrence.bsky.social · 02/12/2023

Are you at #Cellbio2023? Let's hang out! I'll be speaking in the Cryo-EM and Cryo-ET in the Discovery of Cellular Mechanisms session about the eIF2B Switch-Helix on Monday at 5:30, and have a poster (P2953) on Tuesday at 1:45! bsky.app/profile/rosa...

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

And also a massive thanks to Peter, Susan, and Dyche Mullins for their support and mentorship throughout the course of this project.

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

Finally, this was a hugely collaborative effort—a huge thank you to the the HDX-MS wizard Sophie Shoemaker , technician extraordinaire Aniliese Deale for stellar biochemistry, and to cryo-EM experts Lan Wang, Smriti Sangwan, and Aditya Anand. Science is more fun when you do it with friends! [10/10]

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

We are excited about the possibilities this opens up both for drug discovery and for increased control over programming ISR signaling in cells. Stay tuned for future work investigating the cellular mechanisms via which chronic ISR signaling drives metabolic dysfunction. [9/10]

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

We propose a model in which the ISR signaling dial is set by the ratio of A-State:I-State eIF2B conformations, which can be tuned by the presence of effectors or by mutating Switch-helix side-chain interactions. [8/10]

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

Endogenously edited cell lines containing rationally designed switch-helix mutations demonstrated that engineering switch-helix conformation can be used to program ISR signaling states in cells. [7/10]

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

Mutational analysis and in vitro biochemical assays probing enzymatic activity, conformation, and eIF2B assembly state confirmed that conformation of our “Switch-Helix” is sufficient to define the global conformation and activity of eIF2B. [6/10]

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

cryo-EM revealed that this helix lies at the fulcrum of the rotational motion and displays two distinct side-chain orientations, suggesting it may have a switch-like nature. [5/10]

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

Hydrogen-Deuterium Exchange-Mass Spectrometry (HDX-MS) revealed dramatic remodeling of a single alpha helix located at the core of the eIF2B complex in response to inhibition by eIF2-P, along with more subtle remodeling across other regions of the complex. [4/10]

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

eIF2B activity is controlled by conformation. Upon stress, the eIF2-P inhibitor prompts eIF2B subunits to rotate around a central axis, resembling the flapping of a butterfly’s wings. How is conformational change coordinated across 10 subunits? 3/10

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

Depending on context, ISR can promote homeostasis or drive age-related disease. These outcomes depend on the activity of eIF2B, a 500-kDa heterodecameric guanine GEF. Stress-responsive inhibition of eIF2B halts translation and prompts expression of stress-responsive genes. 2/10

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Rosalie Lawrence @rosalielawrence.bsky.social · 09/11/2023

It’s out! Via hydrogen deuterium exchange, biochemistry, and cryo-EM, we (Peter Walter lab, Susan Marqusee lab et al) discovered the allosteric mechanism controlling activity of the core Integrated Stress Response (ISR) regulator eIF2B. 1/10 www.nature.com/articles/s41...

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