iiinova — where your digital self keeps evolving

Roman Doll @romanmdoll.bsky.social · 27/02/2026

Just got my first @plasmidsaurus.bsky.social RNASeq data back and am genuinely blown away🤯 Incredibly easy process, and high-quality data back within 7 days, even from here in the UK!

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Reposted by Roman Doll

Fillip Port @crisprflydesign.bsky.social · 29/01/2026

Make your gene knockouts more efficient with multiplexed Cas12a sgRNAs. Our new paper is out now, with tools available from @addgene.bsky.social , www.plasmids.eu and @vdrc-flies.bsky.social. www.nature.com/articles/s41... #CRISPR #geneediting #Drosophila 🧪🧬✂️🔬🪰 Summary 🧵 below.

Header image with the paper title: "Improved in vivo gene knockout with high specificity using multiplexed Cas12a sgRNAs"

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Roman Doll @romanmdoll.bsky.social · 20/12/2025

Excited to share that a part of my PhD work is online now on BioRxiv! www.biorxiv.org/content/10.6... We developed a genome editing approach to target the JAK2 V617F mutation and demonstrate its potential to alleviate MPN hallmarks in primary patient cells. Thread 👇

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Roman Doll @romanmdoll.bsky.social · 06/11/2025

Check out the latest work from the @davieslab.bsky.social led by Hangpeng!

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Reposted by Roman Doll

Leopold Parts @leopoldparts.bsky.social · 15/01/2025

We are happy to share our enhancer scramble story, a strategy to create hundreds of stochastic deletions, inversions, and duplications within mammalian gene regulatory regions and associate these new architectures with gene expression levels 🧵 www.biorxiv.org/content/10.1...

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Fillip Port @crisprflydesign.bsky.social · 02/12/2024

Our new preprint is out - please share! We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals. Grab a ☕ and let’s dive in. 🧵🧪 1/n #CRISPR #geneediting

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Roman Doll @romanmdoll.bsky.social · 20/11/2024

Feels like the migration of science twitter is actually working this time? Exciting!

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Roman Doll @romanmdoll.bsky.social · 11/11/2024

Some quite interesting observations on length bias of different PCR polymerases, from www.nature.com/articles/s41... Maybe I need to reconsider my unconditional love for Q5...

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