Parallel Squared Technology Institute @parallelsq.bsky.social · 22/09/2026Single-cell proteomics is beginning to reveal functional coordination and principles. This motivate us to keep advancing the depth, quantitative accuracy, and scalability of the technology to further empower this analysis and help identify biological principles and targetable disease mechanisms. 011
Parallel Squared Technology Institute @parallelsq.bsky.social · 16/09/2026A recent Nature Technology Feature (lnkd.in/gurhMjXB) traces the remarkable development of single-cell proteomics, from a technology that was once considered nearly impossible to a field in which it is possible to measure 1000s of proteins from individual cells. See lnkd.in/g2tKtaxJ for more info!lnkd.inLinkedInThis link will take you to a page that’s not on LinkedIn 000
Parallel Squared Technology Institute @parallelsq.bsky.social · 15/09/2026Great to see multiplexed single-cell proteomics uncover new biology. We are looking forward to further increasing the plex and thus the power of the technology for biological discoveries! Congratulations to Luke Khoury and team ! 011
Reposted by Parallel Squared Technology InstituteConvergent Research @convergentresearch.bsky.social · 09/09/2026Excited to see what @slavov-n.bsky.social and @parallelsq.bsky.social have been working on in @nature.com's new article on single-cell proteomics. 022
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 09/09/2026The piece revisits the early days, our lab's initial work alongside other groups pioneering complementary approaches, together showing that mass-spec-based single-cell proteomics was feasible & promising. The proof-of-concept set the field in motion. 2/ www.nature.com/articles/d41...nature.comProbing the proteome at cellular scaleRapid technological advances are allowing researchers to identify thousands of proteins in individual cells, revealing the hidden mechanisms underlying development and disease. 121
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 09/09/2026Nature just published a tech feature on single-cell proteomics. It highlights our journey from skepticism to robust technology and biological discoveries. I love that the feature starts with a biological discovery enabled by the technology: www.cell.com/cell/fulltex... 1/ 133
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 07/09/2026By regulating protein stability, liver cells set protein accumulation rates and thus spatial abundance. Any tissue with a spatial gradient could be using this mechanism. Could this be a general mechanism for sculpting spatial protein gradients ? nikolaislavov.substack.com/p/sculpting-... 052
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 01/09/2026These results bode well for the feasibility of massively scaling up single-cell proteomics while preserving and increasing the depth of coverage and quantitative accuracy. 1/ 152
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 01/09/2026We designed it as an open-source platform that the community can use to invent new acquisition strategies, experiment with creative designs, and develop the next generation of quantitative proteomics. 4/ 🔗 www.parallelsq.org/JMod 031
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 01/09/2026Accurately quantifying over 700K precursors in a single 9-plexDIA set of 20ng proteomes is a new milestone. JMod achieves high quantitative accuracy over a 32-fold dynamic range of proteome spiked-in ratios across single-cell and bulk sample sizes. 1/ doi.org/10.1101/2025... 153
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 13/08/2026Save the date: The 10th Single Cell Proteomics Conference (SCP2027) will be on July 12 - 14, 2027 single-cell.net/proteomics/s... 044
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 08/08/2026Developing treatments for Alzheimer’s and Parkinson’s diseases is limited by the incomplete understanding of the molecular processes underlying them. Single-cell proteomics is opening a new window into these diseases: - It allows for a different type of analysis, different in kind. 1/ 192
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 05/08/2026We're looking for mass spectrometry enthusiasts who thrive on quantitative rigor, innovation and enjoy challenges: - Colleagues eager to propel biological discoveries by inventing the technologies of the future. 168
Parallel Squared Technology Institute @parallelsq.bsky.social · 27/07/2026Twenty-four researchers gathered at PTI for a full day of hands-on learning, collaboration, and discovery at the 2026 Single-Cell Proteomics Workshop. Thank you to everyone who joined us and helped make the workshop a success! www.parallelsq.org/blog/2026-si... 010
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 22/07/2026My Biemann Medal lecture is now available on YouTube. In it, I trace the exhilarating journey of developing single-cell proteomics, beginning on Christmas Day in 2010, when, as a PhD student, I became convinced that understanding biology would require measuring proteins. youtu.be/z0dIhG4C3qI?...youtu.beBiemann Medal Lecture: An exhilarating journey towards single-cell proteomicsYouTube video by Nikolai Slavov 142
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 24/06/2026Since the 1960s, the genetic code has been used to predict protein sequences from DNA and mRNA sequences. Our @nature.com article demonstrates that these predictions miss thousands of protein sequences present in human tissues. www.nature.com/articles/s41... 1/ 28337
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 25/06/2026As usually, the Single-Cell Proteomics Conference aims to maximize accessibility. Thus, #SCP2026 will enable virtual attendance via Zoom. It's free but requires registration at single-cell.net Only presentations by speakers who consent to Zoom broadcast will be included in the virtual program. 165
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 15/06/2026What are the biggest opportunities in biology today? Understanding the proteome is a huge one. It motivated us to launch @parallelsq.bsky.social: > To increase the sensitivity, scale and resolution for exploring one of biology's most exciting frontiers: youtu.be/9Gz6km1J9Wo?...youtu.beThe Proteome: Biology's Next FrontierYouTube video by Nikolai Slavov 171
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 09/06/2026The program for #SCP2026 is online. The talks span diverse technologies and biological questions. Join the discussions, flash talks, and hands-on workshop. 🗓️ July 14-16, 2026 | Boston, USA 🔗 Check out the full program: single-cell.net/proteomics/s... 094
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 05/06/2026It's wonderful sharing the journey with a collaborative and thoughtful community. I'm deeply grateful to all who have contributed to the research recognized by the Biemann medal, a very special distinction for research that is gaining momentum. Let's opens new perspectives and explore together ! 021
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 05/06/2026The annual meeting of the American Society For Mass Spectrometry (@asms.org) has been very special for me as the Biemann Medal recognized our contributions to developing single-cell proteomic technologies and using them to open new perspectives. 2204
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 12/05/2026The final version of our @annualreviews.bsky.social article is now published OA: www.annualreviews.org/content/jour...annualreviews.orgSingle-Cell Proteomic Technologies: Tools in the Quest for PrinciplesOver the last decade, proteomics analysis of single cells by mass spectrometry transitioned from an uncertain possibility to a set of robust and rapidly advancing technologies supporting the accurate ... 0179
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 09/05/2026These numbers are key to understanding RNA and protein analysis. The different counting statistics fundamentally shape technological challenges and opportunities. www.nature.com/articles/s41... 1256
Reposted by Parallel Squared Technology InstituteASMS @asms.org · 08/05/2026Congratulations to Nikolai Slavov, recipient of the 2026 ASMS Biemann Medal! This award recognizes significant early-career achievement and highlights emerging leaders shaping the future of #massspectrometry. #TeamMassSpec 0185
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 29/04/2026I am deeply honored to have been selected to receive the Biemann Medal by the American Society For Mass Spectrometry (@asms.org). While this award is presented to an individual, it reflects the collective contributions of exceptional teams at @slavovlab.bsky.social and @parallelsq.bsky.social. 2232
Parallel Squared Technology Institute @parallelsq.bsky.social · 03/04/2026With years of research at its foundation, Nikolai and Harrison highlight the inspiration supporting PTI's own moonshot to make single cell proteomics scalable and accessible for researchers worldwide. Thanks @cowboybearninja.bsky.social for the video! youtu.be/9NNvkJYa43cyoutu.beUnfolding PTI's Origin Story with Nikolai Slavov and Harrison SpechtYouTube video by Parallel Squared Technology Institute 011
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 31/03/2026This July 14 - 15, the 9th Single-Cell Proteomics Conference (single-cell.net) brings together a community that is redefining what’s possible: Robust methods enable scalable proteoform measurements from single cells without sacrificing depth or quantitative accuracy. 1/3 165
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 04/02/2026You can help enable the quantification of thousands of samples/day without sacrificing depth or accuracy. What makes this position special is that these technologies are directly and tightly integrated with biological discovery. 2/2 jobs.lever.co/convergentre... 141
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 04/02/2026A special opportunity for lovers of mass spectrometry proteomics to join a like-minded team at PTI. Join a collaborative initiative to enable direct protein analysis at unprecedented scale, in partnership with leading instrument developers, academics, and industry leaders. 1/2 1129
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 02/02/2026We’re testing conceptual hypotheses, dissecting disease progression, proteostasis, protein degradation, PTMs, and aggregation, all with focus on creative, rigorous data interpretation. If you want to do science that changes how we think, this is it. 👇 jobs.lever.co/convergentre... 032
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 02/02/2026🔥 We’re hiring! A rare opportunity to rethink neurodegeneration: 🟦 Develop creative, rigorous approaches to data interpretation toward mechanistic insights. Join our team at PTI to study neurodegenerative disease using direct protein analysis at single-cell resolution. 1/2 144
Reposted by Parallel Squared Technology InstituteIonOpticks @ionopticks.com · 12/01/2026During his graduate studies, Mahlon Collins worked with ALS patients who underwent painful spinal taps to donate CSF for his biomarker research. Now, he leads neurodegenerative disease research at @parallelsq.bsky.social. Learn more about Mahlon's journey: bit.ly/3NoKX4gbit.lyFrom patient donations to cellular insights: Mahlon Collins on single-neuron proteomics and the future of Alzheimer’s researchMahlon Collins leads neurodegenerative disease research at the Parallel Squared Technology Institute (PTI). A neurobiologist by training, he has witnessed firsthand the tremendous burden these disease... 022
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 20/01/2026Single-cell proteomics is moving past descriptive snapshots to quantitative models of cellular regulation and biological mechanisms. Come to #Boston this July to shape the next chapter and have your voice heard. Abstract deadline: Apr 1, 2026. single-cell.net/proteomics/s... 095
Parallel Squared Technology Institute @parallelsq.bsky.social · 13/01/2026We're delighted to have Mahlon Collins featured in an @ionopticks.bsky.social article! The article goes over Mahlon's work on AD research, with a focus on advancing single-neuron proteomics at PTI. ionopticks.com/2026/01/09/f... 010
Parallel Squared Technology Institute @parallelsq.bsky.social · 06/01/2026The focussed research organizations enabled by @convergentresearch.bsky.social are driving forward an ambitious agenda ! youtu.be/L5W-lV1K_xQ?...youtu.beConvergent Research: A mission control for frontier technologyYouTube video by Convergent Research 043
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 06/01/2026Biology’s earliest decisions are written in proteins. Using single-cell proteomics, we discovered protein (and functional) symmetry breaking in mammalian embryos long before major transcriptome differences are detectable. cell.com/cell/fulltex... 0142
Parallel Squared Technology Institute @parallelsq.bsky.social · 08/12/2025Single-cell proteomics enables discoveries! It illuminates the frontiers of biomedical research. 010
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 08/12/2025Among all proteome changes in Alzheimer’s disease (AD), 20S proteasomes stand out. ⬛️ Their abundance strongly declines in AD patients. => This decline parallels a reduction in 20S substrate proteins and leads to abnormal protein accumulation in AD. biorxiv.org/content/10.1... 032
Parallel Squared Technology Institute @parallelsq.bsky.social · 01/12/2025To facilitate technology dissemination, we will host a workshop in our laboratory. Register to secure a spot! Workshop participation is free with in-person conference registration but requires an advanced registration. To facilitate interactions and hands-on experience, participation is limited. 020
Parallel Squared Technology Institute @parallelsq.bsky.social · 26/11/2025Research Fest highlighted PTI's scientific achievements to date as well as the team that made it all happen. We are thankful for the members on our team and the community who support our work, and wish everyone a Happy Thanksgiving. youtu.be/YFfeJqHC3tMyoutu.bePanel Discussion with Nikolai Slavov, Harrison Specht, Sarah Sipe, and Kevin McDonnellYouTube video by Parallel Squared Technology Institute 000
Parallel Squared Technology Institute @parallelsq.bsky.social · 20/11/2025The multiplexing approaches that we are developing allow scaling up such rate measurements. timePlex, PSMtags, and JMod can all support increased throughput for 𝑖𝑛 𝑣𝑖𝑣𝑜 metabolic pulse experiments, and thus make dynamic protein analysis more scalable. Quote 101
Reposted by Parallel Squared Technology Institute🎶Jaaane says... 🎶 🇨🇦 @cportgrrl.bsky.social · 19/11/2025More excellent work from @parallelsq.bsky.social ! So proud to be part of this team #TeamMassSpec #proteomics 021
Parallel Squared Technology Institute @parallelsq.bsky.social · 13/11/2025No one is immune to aging but what exactly happens to our immune system as it ages? @thibaultcolombani.bsky.social takes a molecular dive into the immune system and talks about understanding age-induced immune altercations through single-cell proteomics. www.youtube.com/watch?v=WUU1... 053
Parallel Squared Technology Institute @parallelsq.bsky.social · 08/11/2025Proteoforms matter: Protein functions change due to sequence variants and diverse modifications regulating their activities. 𝐓𝐨 𝐬𝐞𝐞 𝐛𝐢𝐨𝐥𝐨𝐠𝐲 (𝐚𝐧𝐝 𝐝𝐢𝐬𝐞𝐚𝐬𝐞) 𝐜𝐥𝐞𝐚𝐫𝐥𝐲, 𝐰𝐞 𝐧𝐞𝐞𝐝 𝐩𝐫𝐨𝐭𝐞𝐨𝐟𝐨𝐫𝐦-𝐥𝐞𝐯𝐞𝐥 𝐪𝐮𝐚𝐧𝐭𝐢𝐟𝐢𝐜𝐚𝐭𝐢𝐨𝐧 𝐚𝐭 𝐬𝐜𝐚𝐥𝐞. 1/2 151
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 01/11/2025𝐶𝑜𝑚𝑏𝑖𝑛𝑎𝑡𝑜𝑟𝑖𝑎𝑙 scaling may unlock a new level of throughput. This is enabling a long-awaited experiment ! Our team, led by Jason Derks, enabled combinatorial-scaling of mass spec proteomics throughput by developing multiplexing in the time domain, timePlex. 1/n 184
Reposted by Parallel Squared Technology InstituteNikolai Slavov @slavov-n.bsky.social · 01/11/2025Combining this time domain multiplexing with mass domain multiplexing (plexDIA), we demonstrated combinatorial scaling of throughput. You can read about it www.biorxiv.org/content/10.1... and hear Jason describe it at the Parallel Squared Technology Institute Research Fest: youtu.be/2rLBk4IaZpk?...youtu.beIncreasing Mass spectrometry throughput using time encoded sample multiplexing | Jason DerksYouTube video by Parallel Squared Technology Institute 132
Parallel Squared Technology Institute @parallelsq.bsky.social · 31/10/2025Mahlon Collins is leading our discoveries in profiling the proteome of single neurons from Alzheimer's disease patients. He presents this research journey in a talk, "Discovering Alzheimer's disease protein dysfunctions with high throughput proteomics". youtu.be/UD8uSIfg7WYyoutu.beDiscovering Alzheimers disease protein dysfunctions with high throughput proteomics | Mahlon CollinsYouTube video by Parallel Squared Technology Institute 010
Parallel Squared Technology Institute @parallelsq.bsky.social · 23/10/2025@jasonderks.bsky.social presents "Increasing Mass spectrometry throughput using time encoded sample multiplexing" at Research Fest in Boston, MA on April 22, 2025. www.youtube.com/watch?v=2rLB...youtube.comIncreasing Mass spectrometry throughput using time encoded sample multiplexing | Jason DerksYouTube video by Parallel Squared Technology Institute 051
Parallel Squared Technology Institute @parallelsq.bsky.social · 16/10/2025And, we are back with another presentation from this year's Research Fest. PTI's Senior Computational Scientist, Kevin McDonnell, talks about developing joint modeling (aka JMod) of mass spectra for empowering multiplexed DIA proteomics. www.youtube.com/watch?v=plK4...youtu.beJmod: Joint modeling of mass spectra for empowering mulitplexed DIA proteomics | Kevin McDonnellYouTube video by Parallel Squared Technology Institute 043
Parallel Squared Technology Institute @parallelsq.bsky.social · 09/10/2025Throwback Thursday continues with a presentation by Maddy Yeh and Mark Adamo from Research Fest: "Tags for improving peptide sequencing and throughput in sensitive proteomics". Watch on YouTube: www.youtube.com/watch?v=jA2I...youtube.comTags for improving peptide sequencing and throughput in sensitive proteomics | Maddy Yeh, Mark AdamoYouTube video by Parallel Squared Technology Institute 021