Sign in

Pablo Carravilla

@pablo-carravilla.bsky.social
306 followers 169 following 8 posts

Group leader at Biofisika Institute biofisika.org Cell membranes, biophysics, microscopy, rare diseases.

PostsRepliesMedia
Reposted by Pablo Carravilla
Itay Budin @ibudin.bsky.social · 24/04/2025
Out today, we report how small chemical modifications in the metabolism of ergosterol are needed for yeast membranes to phase seperate into ordered yet fluid domains www.science.org/doi/10.1126/... (1/n)
science.org
Structural dissection of ergosterol metabolism reveals a pathway optimized for membrane phase separation
The metabolism of ergosterol shapes this lipid to allow the formation of fluid membrane domains in yeast cells.
37524
Reposted by Pablo Carravilla
Levental Lab @leventallab.bsky.social · 19/05/2025
Hey gang, stop what you’re doing, new organelle just dropped!! Amazing the kind of stuff one finds inside cells by just looking at them (with magic like cryoET). What the heck are all these strange hemifused vesicles?
1353
Reposted by Pablo Carravilla
Fabio Lolicato @fabiololicato.com · 06/03/2025
🚨 Postdoc Opportunity #2 🚨 We are looking for candidates with a strong background in molecular dynamics simulations of membrane protein interactions to unravel the role of lipids in CD95 oligomerization and signaling! Please apply by March 28! #LipidTime @bzh-hd.bsky.social
0139
Reposted by Pablo Carravilla
Dimova Lab @dimovalab.bsky.social · 26/02/2025
📢 SAVE THE DATE: Sept 29 - Oct 1, 2025 and come to Berlin to the BIOMEMBRANE DAYS Excellent line of speakers and slots for contributed talks. Registration will open in Spring. biomembrane-days-2025.mpikg.mpg.de Organized by Helge@ewerslab.bsky.social Thomas Weikl and myself @mpici.bsky.social
02114
Reposted by Pablo Carravilla
Erdinc Sezgin @sciezgin.bsky.social · 19/02/2025
New paper out 🎇 It has been 13 years since @owenlabuob.bsky.social and co. published a stellar protocol on how to measure membrane order with smart probes! Since then, there has been new dyes, imaging modes and biology! We wrote a protocol for Nature Protocols: www.nature.com/articles/s41...
nature.com
Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols
Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...
17323
Pablo Carravilla @pablo-carravilla.bsky.social · 19/02/2025
We wrote a protocol to quantify plasma membrane fluidity using a confocal microscope 🔬 Hopefully those interested in measuring membrane biophysical properties in live cells find it useful. We also provide an ImageJ/Fiji tool to make the analysis as easy as possible. www.nature.com/articles/s41...
nature.com
Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols
Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...
161
Reposted by Pablo Carravilla
Gerry Hammond @gerryhammond.bsky.social · 11/02/2025
The most frequently asked question we get: do #lipidtime genetically encoded lipid biosensors compete out cells’ native effector proteins? Victoria Holmes and Morgan Ricci led a paper in JCB that answers this question: rupress.org/jcb/article/... Read on for a brief thread.
rupress.org
Single-molecule lipid biosensors mitigate inhibition of endogenous effector proteins | Journal of Cell Biology | Rockefeller University Press
Holmes and colleagues show that AKT activation is blocked by commonly used PIP3 biosensors, but that this can be overcome by expressing biosensors at singl
175
Pablo Carravilla @pablo-carravilla.bsky.social · 26/01/2025
Thanks for your support throughout my research journey Erdinc, you have been a true inspiration for me!
030
Reposted by Pablo Carravilla
Falk Schneider @faldalf.bsky.social · 16/12/2024
How does nano-scale plasma membrane structure underpin tissue development? Let’s find out! First opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick. Fully funded studentship by #MIBTP and University of Warwick. 🔬✨🐟📸 More info here: www.findaphd.com/phds/project...
Temporal projection of a microscopy time-lapse image. Membranes of the cells in zebrafish brain at 20 hpf are labelled. Time is colour coded.
01512
Reposted by Pablo Carravilla
Falk Schneider @faldalf.bsky.social · 13/12/2024
‼️3 postdoc positions open‼️ We are building a quantitative imaging team at Warwick! Join an interdisciplinary endeavor with Mishima, Balasubramanian, and Mayor labs on a @wellcometrust.bsky.social funded project to push probe and microscopy development! warwick-careers.tal.net/vx/lang-en-G...
media.tenor.com
a logo that says let 's do this in blue and yellow
ALT: a logo that says let 's do this in blue and yellow
22621
Reposted by Pablo Carravilla
Julian Kompa @juliankompa.bsky.social · 27/11/2024
Hi Bluesky! 👋 Here is our latest collaborative work on long-term (up to 20 min) single-molecule tracking in living cells using exchangeable HaloTag Ligands 🔬 #ChemBio #microscopy onlinelibrary.wiley.com/doi/10.1002/...
onlinelibrary.wiley.com
Long‐Term Single‐Molecule Tracking in Living Cells using Weak‐Affinity Protein Labeling
A self-labeling protein tag in combination with exchangeable fluorophore ligands bypasses photobleaching and enables long-term single-molecule imaging in living cells. This extends observation times ...
1348
Reposted by Pablo Carravilla
Joachim Goedhart @joachimgoedhart.bsky.social · 25/11/2024
The dark side of fluorescent protein tagging: the impact of protein tags on biomolecular condensation www.biorxiv.org/content/10.1...
Figure 4. Position and type of fluorescent tag affect protein expression and P-body numbers in vivo.
10305119