Background The gut microbiome has been widely studied in the context of obesity, and yet the reported associations vary widely across populations and analytical approaches. In Asian populations where ...

NUHS researchers use a genetic fingerprint to expose invisible microbial reservoirs, aiding outbreak prevention across local hospitals.

sm-PORE-cupine combines SHAPE-based chemical probing with nanopore-based direct RNA sequencing to identify RNA structural ensembles in the SARS-CoV-2 genome and the Candida albicans transcriptome.

Redirecting to https://csb5.github.io/ ...

Nature Cancer - Nagarajan and colleagues overview the current challenges in microbiome analyses from human cancer samples and discuss the optimal practices for improving the standards of reporting...

Nature Cancer - Nagarajan and colleagues overview the current challenges in microbiome analyses from human cancer samples and discuss the optimal practices for improving the standards of reporting...

Despite being important ecological modulators of the gut microbiome, bacteriophage diversity and function remain under-characterized. We show that short-read metagenomic surveys can miss even globally highly prevalent viral family-level clusters (VFCs), that can be readily assembled and characterized with long-read metagenomic data from a relatively small cohort (n=109). While gut Bacteroidota phages have been the prevailing focus in the literature, we show that highly prevalent gut phage families frequently have Firmicutes hosts (termed GuFi phages), with broad host ranges verified using proximity-ligation (Hi-C) sequencing data. High-throughput sequencing of virus-like particles from fecal samples detected frequent enrichment of GuFi phages across samples, revealing their under-appreciated impact on the gut microbiome. We report the first in vitro induction and imaging of members of prevalent GuFi clades including the candidate orders Heliusvirales , Astravirales (VFC 2) and Suryavirales (VFC 4). Our findings underscore the importance of GuFi phages with broad host ranges in the gut microbiome, and the utility of long-read sequencing for viral discovery, paving the way for deeper insights into the role of bacteriophages in human health and disease. ### Competing Interest Statement IL is an employee of Phase Genomics. National Medical Research Council, 23-0614 National Research Foundation, NRFI09-0015 A*STAR, C210812044

The field of high-throughput microbial culturomics is still in its early stages. Enhancing our ability to isolate and phenotypically test bacterial strains from complex communities is crucial for adva...

npj Antimicrobials and Resistance - Citywide metagenomic surveillance of food centres reveals local microbial signatures and antibiotic resistance gene enrichment

Skin metagenomic and metatranscriptomic analysis shows divergence between microbial abundance and activity.

Skin metagenomic and metatranscriptomic analysis shows divergence between microbial abundance and activity.

Perez, Kimoto, Rajakumar and colleagues present a fast and accurate DNA sequencing method that reads canonical and non-canonical bases using AI and nanopore technology. The approach enables an expande...

In this Consensus Statement, the authors outline strategies for processing, analysing and interpreting low-biomass microbiome samples, and provide recommendations to minimize contaminants.

The human skin microbiome is increasingly recognized to influence skin health, immune function and disease susceptibility. However, large-scale, multi-site metagenomic studies in the general populatio...