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Reposted by Nicole Hoitsma

Bret Freudenthal @freudlab.bsky.social · 05/09/2025

Thrilled to share our lab’s latest work in JBC! 🎉 We uncovered a critical role for APE1 Asn174 in stabilizing AP-sites using mutagenesis, enzymology, crystallography, and MD-simulations. Congrats to Kaitlin Dehart and all the co-authors! Check it out: www.jbc.org/article/S002...

jbc.org

APE1 active site residue Asn174 stabilizes the AP-site and is essential for catalysis

Apurinic/Apyrimidinic (AP)-sites are common and highly mutagenic DNA lesions that can arise spontaneously or as intermediates during Base Excision Repair (BER). The enzyme apurinic/apyrimidinic endonu...

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Reposted by Nicole Hoitsma

Karolin Luger @nucleosomepolice.bsky.social · 18/11/2024

Our paper on the strange ATP-dependent chromatin remodeler Smarcad1 paper is out and freely accessible. Differential regulation of its many activities by phosphorylation. Congrats to Briana and @nhoitsma.bsky.social 🧪https://www.sciencedirect.com/science/article/pii/S0021925824023950?via%3Dihub

sciencedirect.com

Phosphorylation regulates the chromatin remodeler SMARCAD1 in nucleosome binding, ATP hydrolysis, and histone exchange

Maintaining the dynamic structure of chromatin is critical for regulating the cellular processes that require access to the DNA template, such as DNA …

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Reposted by Nicole Hoitsma

Karolin Luger @nucleosomepolice.bsky.social · 09/10/2024

Not to take anything away from the fantastic scientists who were recognized this year, and unreservedly applauding the choices.... but i guess we are back to all guys now for the STEM Nobels. Just an observation..... 🧪👩‍🔬

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