We show deletion of an IS200-encoded sRNA (5′tnpA) in Salmonella impacts the expression of SPI-1, flagellar, cysteine, and phsABC regulons. Evidence is presented that, with regard to the SPI-1 regulo...

The enteric bacterium Enterococcus faecalis incorporates fatty acids from the medium and elongates any short chains to lengths suitable for membrane lipids. We have delineated the pathway.

Disease-related Fig4 mutants displaying impaired association with the Fab1-Vac14-Fig4 complex confer rapamycin tolerance in Saccharomyces cerevisiae independent of Vac14 and Fig4 catalytic function. ...

Riboswitches are RNA molecules that control gene expression and have been hypothesized to function independently of proteins. The monofunctional regulator RibR from the bacterium Bacillus amyloliquef...

The destabilization of mitochondria due to the disruption of the Lon1 protease induces cellular damage, including reactive oxygen species (ROS) accumulation, impaired germination, and growth arrest. ....

Protein homeostasis is perturbed at extremely high growth temperatures and without molecular chaperones or proteases. The protein synthesis-inhibiting branch of the stringent response involves elevat...

The periplasmic multicopper oxidase CueO oxidizes cuprous ions (Cu+) to cupric ions (Cu2+), a well-known process typically coupled with oxygen reduction. In this study, we report unexpected anaerobic...

Chitin synthases exhibit distinct protein structures and functional diversity, resulting in differential regulatory effects on the growth and secondary metabolism of M. purpureus. An integrated appro...

When Streptococcus pneumoniae produces low amounts of the second messenger cyclic di-AMP (c-di-AMP), they do not survive in the presence of competence-stimulating peptide (CSP), which is used for tra...

The HWE-family histidine kinase PhyK regulates the cell envelope structure of the intracellular pathogen Brucella ovis and affects how the host immune system responds to this bacterium. Deletion of p...

Growing evidence points to the vital role of communication among parasites in coordinating behavior and promoting functional specialization. These interactions significantly impact drug resistance, c...

In Hyphomicrobium denitrificans, the use of thiosulfate as a supplemental electron donor is regulated by two homologous sulfane-sulfur responsive ArsR-type transcriptional repressors, sHdrR and SoxR,...

Mtb transposon sequencing database is a unified resource for exploring conditional gene essentiality in Mycobacterium tuberculosis. By standardizing over 150 transposon sequencing screens, it enables...

COL is an unusual “model” strain of Staphylococcus aureus that exhibits slow growth and multidrug antibiotic tolerance. This phenotype is primarily due to a mutation in Prs, which synthesizes the cor...

FliS, a flagellin protein chaperon of the Lyme disease pathogen Borrelia burgdorferi, controls the cytoplasmic pool of flagellins (FlaB) at the level of translation initiation, secretion, and proteol...

An overview of resistance mechanisms in two East Asian Candida auris isolates evolved in vitro. The A1.1 strain acquired resistance by enhancing oxidative stress responses through the AOX2, RTG3, and...

FlhG (orange, with activator helix in green) regulates peritrichous flagellation in Bacillus subtilis by interacting with the C-ring protein FliY (gray, N-terminus in red) and the cell wall regulator...

Pseudomonas syringae swimming motility is induced by proximity to other species of bacteria, leading to the formation of asymmetric colonies. Upper panels show examples of P. syringae and mutants gro...

Iron is essential for life, yet excessive levels can quickly lead to cellular damage via oxidative stress, underscoring the need for global regulators of iron and heme homeostasis. In this study, we ...

A novel approach using the Pseudomonas syringae ice nucleation protein (INP) system to present Borrelia burgdorferi lipoproteins on the surface of Escherichia coli reveals conservation of complement ...

During nitrogen fixation, the methanogen Methanosarcina acetivorans produces the alternative vanadium-nitrogenase and iron-nitrogenase only when molybdenum is unavailable to support the activity of m....

5-Oxoproline (OP) is a toxic by-product of metabolism generated by all organisms. Since OP is ubiquitous, oxoproline detoxification systems are found throughout nature. We demonstrate that the Clostr...

This study shows that PldX, a luminal Phospholipase D, is necessary for the efficient destruction of ingested Klebsiella pneumoniae bacteria in Dictyostelium discoideum phagosomes. PldX and the AlyL ...

Using bioinformatic tools, we identified a diverse defensome in Actinobacillus pleuropneumoniae, featuring various defense mechanisms. Genomes show co-occurring systems like CRISPR, Abi, and restrict....

A novel TetR-like regulator (EppR) has been identified to repress genes encoding DNA polymerase V in Acinetobacter baumannii through the direct binding of a conserved EppR motif in their promoters. E...

In biofilms, genetically identical cells exhibit diverse gene expression, resulting in subpopulations of functionally distinct and coexisting cell types, such as motile cells, extracellular matrix pr....

Blastocrithidia represents a very small group of species, in which all 3 stop codons are reassigned to encode amino acids with only one of them serving a dual role of a sense and a stop codon simulta...

Porphyromonas gingivalis exports several virulent proteins, including gingipain proteinases, via the type 9 secretion system. Expression of several T9SS component proteins is regulated by the PorA-Po...

Nucleoid-associated proteins play a crucial role in the compaction of bacterial DNA and the regulation of gene expression. The emergence of small noncoding RNAs as regulatory elements in these proces....

ScrM is a novel competence-induced peptidoglycan hydrolase with activity against pyogenic streptococci. ScrM binds specifically to midcell of sensitive bacteria. Immunity to ScrM is mediated by a ded...

Immunofluorescence microscopy of a large parasitophorous vacuole of Plasmodium developing in hepatocytes for 48 h showing Golgi vesicles (GRASP-GFP) and the apicoplast (ACP in red). DAPI, nuclei.

In this study, Barretto and Fowler investigate how the properties of Salmonella's PhoPQ two-component system have evolved to facilitate its role as a master regulator of virulence gene expression whe...

Biochemical data on the isolated Na+/H+-exchanging ATPase, PfATP4, from the malaria parasite are missing. Here, we expressed and purified the nucleotide and phosphate binding domains as a soluble pro....

Molecular oxygen diffuses across membranes with such speed that, despite respiration, the intracellular oxygen concentration does not differ significantly from that outside the cell. In contrast, mem...

The Roc system represents a highly interconnected but incomplete network of two-component regulatory systems involved in the virulence of Pseudomonas aeruginosa. Our work has enabled us to identify t....

(A) MecA is known as an adaptor of the ClpCP protease. It targets its substrates like alternative sigma factor σx and presents them to ClpCP for degradation in regulation of competence development in....

Molecular Microbiology is a broad-scope microbiology journal for basic and translational research that contributes to understanding molecular principles in microbes.

Proposed model in which human miR-6762-5p expression activates RhoA to promote Shigella flexneri dissemination: During the late phase of infection, miR-6762-5p is expressed from its host gene TPCN1. ...

In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of ....

Molecular Microbiology is a broad-scope microbiology journal for basic and translational research that contributes to understanding molecular principles in microbes.

Polyphosphate condensates and their contributions to nucleoid structure. Polyphosphate condensates form in the ribosome-depleted nucleoid region of bacterial cells, particularly under stress (in this...

NrdR in Streptococcus and Listeria spp. regulates the expression of ribonucleotide reductase genes, which produce DNA building blocks. We show that NrdR binds DNA in an ATP/dATP-dependent manner, and...