This paper shows that high MOI sgRNA multiplexing maintains CRISPRi screen performance while enabling reduced cell numbers, challenging conventional reliance on low-MOI infections.

Applications to be submitted by October 28, 2025 Compensation Grade: P99 (Research Affiliate I) Compensation Details: Minimum: $62,353.20 - Maximum: $62,353.20 Annually Positions with a designated wor...

Applications to be submitted by October 13, 2025 Compensation Grade: P99 (Research Affiliate I) Compensation Details: Minimum: $62,353.20 - Maximum: $62,353.20 Annually Positions with a designated wor...

Cryptococcus neoformans is the top-ranked World Health Organization priority fungal pathogen due to its widespread distribution and inadequate treatment options. Additionally, as a basidiomycete yeast...

Cryptococcus neoformans is the top-ranked World Health Organization priority fungal pathogen due to its widespread distribution and inadequate treatment options. Additionally, as a basidiomycete yeast...

The construction and high-precision fitness profiling of a C. neoformans knockout library reveals a high-resolution functional map of its genome and allowed for the identification of fungal analogs of...

Abstract. We recently described CRISPR/Cas9-based short homology-dependent genome engineering in the human fungal pathogen Cryptococcus neoformans, a haplo

Abstract. Cryptococcus neoformans is the top-ranked W.H.O. fungal priority pathogen, but tools for generating conditional mutations are limited. Auxin-indu