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Michalis Nikolaidis

@mg-nikolaidis.bsky.social
157 followers 190 following 48 posts

Professor I Trying to speak my mind about the relevance of molecular processes in physiology and nutrition I Reviews Editor in Eur J Appl Physiol | nikolaidis.weebly.com

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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 07/09/2026
Eric Trexler and Eric Helms devoted most of an episode of their podcast to our preprint on the energetic cost of building 1 kg of human skeletal muscle. They went through the assumptions one by one, then asked what the number might be good for. www.youtube.com/watch?v=ua2s...
youtube.com
Ep 387 - Energy Cost of Building Muscle
YouTube video by Iron Culture Podcast
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 22/08/2026
1/13 How many calories does it take to build 1 kg of muscle? The question is straightforward but, as far as we can tell, it has not yet been answered quantitatively. www.biorxiv.org/content/10.6...
biorxiv.org
The Energetic Cost of Building Human Skeletal Muscle
The energetic cost of building human skeletal muscle has never been explicitly calculated or measured. We developed a quantitative bottom-up accounting model that integrates human skeletal-muscle comp...
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 03/05/2026
When vitamin C levels are low, correcting them makes sense. But in this study, we found no evidence that each person needs a personalized vitamin C supplementation.
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Reposted by Michalis Nikolaidis
Trends in Molecular Medicine @cp-trendsmolecmed.bsky.social · 03/10/2025
ICYMI: ONLINE NOW: Science captured by storytelling: the oxidative stress narrative
dlvr.it
Science captured by storytelling: the oxidative stress narrative
The oxidative stress paradigm stands as a textbook example of how a simplified, early, and appealing scientific concept can become canonized and then resist revision. What began as a plausible heuristic about oxidative stress evolved into a research agenda and a pervasive cultural script.
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 05/10/2025
Is “oxidative stress” a useful concept in biology and medicine? In this work, led by Nikos, we argue that the term “oxidative stress” is devoid of biological meaning and, even worse, misleading.
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 08/08/2025
Meta-research in muscle science: an audacious study shows glycerol storage, used for >60 years, makes fibers stiffer. Other biochemical effects? An important finding that may force a reexamination of past studies and influence the design of new ones. rupress.org/jgp/article/...
rupress.org
Glycerol storage increases passive stiffness of muscle fibers through effects on titin extensibility | Journal of General Physiology | Rockefeller University Press
To store and preserve muscle function, glycerol is often used, but its effect on passive properties is unclear. Muscles stored in glycerol showed increased
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 05/08/2025
How much researchers truly believe what they are saying (from most to least): 1. Conference chat after some beers 2. Conference presentation 3. Paper (first draft) 4. Paper (published) 5. Grant proposal 6. Interview to media 😅
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 11/07/2025
My understanding is that increases in blood reactive species or ox. stress markers cannot, predominantly, originate from ox. stress in tissues, since neither reactive species nor ox. stress products generated in cells have been shown to exit tissues & contribute to blood levels.
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 10/07/2025
A fresh perspective on hydrogen peroxide metabolism during exercise. In vivo (normoxic), muscle contractions typically cause only minimal increases in H₂O₂ (<10%), whereas in vitro (hyperoxic) assays often show far greater increases (~150–340%). sciencedirect.com/science/arti...
sciencedirect.com
State of the art in vivo reactive oxygen species measurements in skeletal muscle using fluorescent proteins
Evaluation of the effects of muscle contractions on reactive oxygen species (ROS) concentrations and their intracellular and intraorganelle dynamics i…
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 07/07/2025
Erythrocyte glutathione synthesis peaks at ~1.0 g protein/kg/day in young & old individuals, even though glutathione concentrations remain unchanged. Additional protein offers no benefit for those with "normal" glutathione levels. ajcn.nutrition.org/article/S000... ajcn.nutrition.org/article/S000...
ajcn.nutrition.org
Protein intake affects erythrocyte glutathione synthesis in young healthy adults in a repeated-measures trial
In 2005, the Institute of Medicine advised using methods other than nitrogen balance (NB) for determining protein requirements. Since then, protein requirements using indicator amino acid oxidation (I...
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 05/06/2025
Much of the joy of computational papers lies in how they bring scattered insights together. Even if you don’t model, this one helps experimentalists design better studies by clarifying how training stresses drive specific adaptations across energy systems. arxiv.org/abs/2503.14841
arxiv.org
The three-dimensional impulse-response model: Modeling the training process in accordance with energy system-specific adaptation
Athletic training is characterized by physiological systems responding to repeated exercise-induced stress, resulting in gradual alterations in the functional properties of these systems. The adaptive...
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 29/05/2025
Vitamin C+E supplementation blunts molecular adaptations to sprint interval training but not performance gains. Since performance wasn’t impaired, I wonder whether, and to what extent, the blunted molecular responses can truly be considered detrimental physoc.onlinelibrary.wiley.com/doi/10.1113/...
physoc.onlinelibrary.wiley.com
Antioxidant supplementation blunts the proteome response to 3 weeks of sprint interval training preferentially in human type 2 muscle fibres
Abstract figure legend Sprint interval training (SIT) is a popular time-efficient type of endurance training. Healthy young men performed nine SIT sessions (4–6 × 30 s all-out cycling sprints) over 3...
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 23/04/2025
James Imlay turns redox chaos into clockwork: his new paper shows how membrane permeability sets the tempo of oxidative stress, with straight-up back-of-the-envelope math in the appendix. Fundamental, elegant, causal. onlinelibrary.wiley.com/doi/10.1111/...
onlinelibrary.wiley.com
The Barrier Properties of Biological Membranes Dictate How Cells Experience Oxidative Stress
Molecular oxygen diffuses across membranes with such speed that, despite respiration, the intracellular oxygen concentration does not differ significantly from that outside the cell. In contrast, mem...
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 15/04/2025
Despite the challenges, this well-conducted study found that chronic Theracurmin—a bioavailable curcumin extract—supplementation neither enhanced nor impaired muscle hypertrophy in mice, but simply modulated some inflammatory and oxidative stress markers. journals.humankinetics.com/view/journal...
journals.humankinetics.com
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 04/04/2025
Eur J Appl Physiol now welcomes reviews from all thinkers—whether or not they identify as physiologists. A great review is not about labels but about curiosity, depth & synthesis. Young scientists, your fresh takes are especially invited. More in my editorial: link.springer.com/article/10.1...
link.springer.com
Review papers that push the boundaries of physiology and EJAP - European Journal of Applied Physiology
European Journal of Applied Physiology -
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 12/02/2025
Study shows that chronic antioxidant supplementation (VitC+VitE) blunted molecular adaptations to training, yet, no impact on any measure of exercise performance—considering that "antioxidant" is a vague term and that VitC+VitE may not always act as such. www.biorxiv.org/content/10.1...
biorxiv.org
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 31/01/2025
Important study highlighting plausible molecular links between redox metabolism and exercise performance—an area notoriously difficult to dissect. The findings challenge assumptions that G6PD deficiency decreases exercise performance. A human replication study would be invaluable.
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Reposted by Michalis Nikolaidis
Nikos Margaritelis @margaritelis.bsky.social · 30/01/2025
Our contribution to the Special Issue "Unlocking Athletic Potential: Exploring Exercise Physiology from Mechanisms to Performance" in FRBM, Guest-edited by Prof. Gomez-Cabrera & @c-handschin.bsky.social #redox #supplements #sports #evidence
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 30/01/2025
A special issue on Exercise Physiology and Redox Biology, with bridges built along the way. Thanks for the kind invitation.
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 06/01/2025
If you are measuring total protein content in skeletal muscle and wondering about the origin of the often-cited 'holy number'—that ~20% of muscle is protein—a recent detailed study provides support for it, reporting a value of 17.7%. journals.physiology.org/doi/full/10....
journals.physiology.org
Human adipose and skeletal muscle tissue DNA, RNA, and protein content | Journal of Applied Physiology
The purpose of this project was to provide a profile of DNA, RNA, and protein content in adipose tissue, which is relatively understudied in humans, to gain more insight into the amount of tissue that...
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 16/12/2024
I believe that a great issue stemming from the undeniable ergogenic potential of carbohydrates and creatine is the misconception that increasing any 'good' molecule in our body will automatically enhance exercise performance—and the reverse.
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 10/12/2024
Remarkable study in mice provides invaluable insights but occasionally falls into the pitfall of overcomparing its findings to human studies, despite being unfeasible in humans. Plus a nuanced review of exercise metabolism, rich with intricacies for researchers. www.sciencedirect.com/science/arti...
sciencedirect.com
Quantification of nutrient fluxes during acute exercise in mice
Despite the known metabolic benefits of exercise, an integrated metabolic understanding of exercise is lacking. Here, we use in vivo steady-state isot…
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 10/12/2024
Different concentrations of the reactive nitrogen species (RONS) nitrate and nitrite in trained vs untrained and healthy vs diseased groups, pointing to significant physiological implications.
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 25/11/2024
A list of (exercise) skeletal muscle researchers in Bluesky
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 25/11/2024
Free radicals and antioxidants: appealing to magic Our short paper argues that molecules cannot be classified as good or evil. Limited evidence supporting the consumption of antioxidants or superfoods and such interventions risk disrupting fundamental redox processes. www.cell.com/trends/endoc...
cell.com
Free radicals and antioxidants: appealing to magic
In biology, there are no good or evil molecules. There is limited or no evidence to support the consumption of antioxidants or (super)foods rich in antioxidants, for the intended purpose of an antioxi...
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 25/11/2024
A list of redox biology researchers in Bluesky
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 23/11/2024
Sabine Hossenfelder and Professor Dave Explains are, in my view, both serious and thoughtful science communicators. Recently, Sabine shared her perspective on why academia is failing: www.youtube.com/watch?v=LKiB...
youtube.com
My dream died, and now I'm here
YouTube video by Sabine Hossenfelder
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Michalis Nikolaidis @mg-nikolaidis.bsky.social · 22/11/2024
Impressive paper by Rob Phillips exploring physical biology through a historical lens: www.cell.com/current-biol...
cell.com
Seeing with an extra sense
In this Guest editorial for the special issue, Rob Phillips considers some of the ways in which viewing the living through a physical lens allows us to see things that might otherwise remain hidden.
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