Reposted by Mari B. Gornitzka
New BCR reconstruction tool for scRNA-seq: assembles full heavy & light chains and annotates constant regions. Check it out:
www.biorxiv.org/content/10.1...
#scRNAseq #immunology
biorxiv.org
GATHeR: Graph-based Accurate Tool for Immunoglobulin HEavy- and Light-chain Reconstruction
Recovering full-length, paired B-cell receptor (BCR) sequences from scRNA-seq reads remains difficult, especially in naive and memory B cells where immunoglobulin transcripts are sparse. Current metho...