iiinova — where your digital self keeps evolving

Reposted by Loucif Remini

GdR ImaBio 🔬 @gdrimabio.bsky.social · 08/01/2026

#ESAB2026 is opening ! 👉 Registration is now available for the second edition of the European South Atlantic Biophysical conference. 👉 esab2026.cbs.cnrs.fr @cnrs.fr @umontpellier.bsky.social @cbsmontpellier.bsky.social @iupab.bsky.social @picoquant.bsky.social @cecamevents.bsky.social

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Reposted by Loucif Remini

Center for Structural Biology (CBS Montpellier) @cbsmontpellier.bsky.social · 05/11/2025

Postdoctoral position in the team of Alessandro Barducci in the CBS, focusing on multiscale simulations of RNA-driven condensates, with applications in synthetic biology. More information : www.cbs.cnrs.fr/images/jobs/... The position is expected to start in early 2026, with some flexibility.

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Loucif Remini @lremini.bsky.social · 30/04/2025

Thanks to my coauthors; Midas Segers, Andrea Parmeggiani and @ecarlon.bsky.social for this nice collaboration.

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Loucif Remini @lremini.bsky.social · 30/04/2025

Taken together, our results support a scenario where interphase chromatin self-organizes into alternating α/β domains. This spatial arrangement likely reflects the combined action of passive mechanisms (e.g. bridging interactions) and active loop extrusion processes.

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Loucif Remini @lremini.bsky.social · 30/04/2025

This memory effect is due to looped domains which introduce periodic trajectories of the chromatin organization. The auxin treatment of cells inhibes actives processes of loop extrusion due to cohesin which leads to the disorganization of the beta phase and thus of the memory effect.

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Loucif Remini @lremini.bsky.social · 30/04/2025

We further analyze non-Markovian effects by computing conditional distance distributions and three-point correlations. These analyses reveal that local chromatin structure influences distal contacts over hundreds of kilobases, with stronger memory effects in WT cells compared to Auxin-treated ones.

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Loucif Remini @lremini.bsky.social · 30/04/2025

To interpret the observed scaling and spatial heterogeneity, we introduce a heterogeneous random walk model. Despite its simplicity, it reproduces key features of the data, including the transition between intra-loop and inter-loop regimes, and the emergent microphase-separated structure

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Loucif Remini @lremini.bsky.social · 30/04/2025

The α phase is consistent with a crumpled globule, a metastable polymer state which is spatially compact. The β phase has a weaker exponent, suggesting a looped organization, possibly in the form of rosette-like domains. This interpretation is supported by experimental data and analytical modeling.

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Loucif Remini @lremini.bsky.social · 30/04/2025

The scaling behavior of these phases is analyzed in detail. We find consistent results across human and mouse data, with distinct exponents for the α and β phases. This allows us to characterize different regimes of chromatin folding across multiple levels of genome organization.

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Loucif Remini @lremini.bsky.social · 30/04/2025

We focus on the probability distributions of pairwise spatial distances between labeled genomic loci. These distributions reveal a robust two-component structure, well described by a superposition of Gaussians. This statistical signature points to the coexistence of two conformations α and β phases

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Loucif Remini @lremini.bsky.social · 30/04/2025

We analyze high-resolution multiplexed FISH (m-FISH) data from human and mouse cells, covering genomic scales from 5 kb to 2 Mb. This allows us to compare and unify chromatin structural features across species and over a wide range of genomic distances.

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Loucif Remini @lremini.bsky.social · 30/04/2025

We have recently published the following article in The Journal of Chemical Physics: “Inferring interphase chromosomal structure from multiplexed FISH data: a unified picture from human and mouse cells” In this thread, I summarize the main findings and ideas discussed in the paper.

doi.org

Inferring interphase chromosomal structure from multiplexed fluorescence in situ hybridization data: A unified picture from human and mouse cells

We analyze multiplexed fluorescence in situ hybridization (m-FISH) data for human and mouse cell lines. The m-FISH technique uses fluorescently-labeled single-s

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Reposted by Loucif Remini

Katie Sandlin @kmsandlin.bsky.social · 14/02/2025

I maxed out my credit card, emptied my savings account, and took out a loan to move from Alabama to Bethesda, MD. I don’t even qualify for unemployment since I’ve only worked at NIH for a month. I will be financially and medically devastated. Seeking suggestions for anywhere that’s hiring!!

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