Population-level nuclear magnetic resonance (NMR)-based lipoprotein profiling is a key tool for investigating dysregulated lipoprotein metabolism and its role in cardiovascular disorders. However, associations with size-resolved lipoprotein composition readouts are difficult to dissect, as these traits are often highly correlated. Derived variables can therefore be more relevant to biological interpretation. Here, we show that the total fatty acid (FA) content of the lipoproteins derived from their lipid headgroup concentrations using the formula FA = 3TG + 2PL + CE strongly correlates (Spearman rho = 0.98) with the independently measured total fatty acid content. This observation is not self-evident since these variables are determined using different portions of the NMR spectrum. Using NMR data acquired on the Nightingale platform for 274,303 UK Biobank (UKB) participants and genetic associations as a readout, we then show that this relationship also holds at the size-resolved lipoprotein level. We identified eight gene loci where the proposed FA variables display a significantly stronger genetic association signal than that of the corresponding lipid headgroup variables. Five of these loci (LIPC, LIPG, PLB1, LPL, and APOC3) have a direct function in FA metabolism. Including computed size-resolved FA variables may therefore improve the biological interpretation of future studies based on Nightingale’s data.

Genome-wide association analyses of blood plasma samples using a mass spectrometry-based platform illustrate the complementarity of different proteomics approaches for identifying protein quantitative...

Accurate quantification of circulating proteins is critical for assessing biological variation and integrating proteomics with other omics to understand biological processes and disease mechanisms. Pr...

We have developed a novel algorithm termed GoldenHaystack (GH) that was designed for enhanced peptide quantification of data-independent acquisition liquid-mass spectrometry (DIA-LC-MS) data files reg...

How to print 3D protein and molecular structures - from virtual to physicalThis is a 3D print of an antibody bound to a protein, i.e. PDGF-B blocking antibody bound to PDGF-BB. Blue and red are two su...

Researchers at Weill Cornell Medicine-Qatar (WCM-Q) have published a study demonstrating the use of an advanced nanoparticle-based tool for analyzing human proteins in order to discover both their fun...

Here, by integrating faecal metabolomics, metagenomics, and habitual dietary data of two large human cohorts, the authors show that faecal metabolites reflect diet and gut microbiome interactions, pre...

Genome-wide association analyses of blood plasma samples using a mass spectrometry-based platform illustrate the complementarity of different proteomics approaches for identifying protein quantitative...

In this Review, Chen et al. discuss how the advancement and integration of large-scale genetic resources, multi-omics data and sophisticated computational tools are improving drug development pipeline...

This Report outlines research Recommendations from the NIDDK’s Working Group of Council on Heterogeneity of Diabetes.

This Report outlines research Recommendations from the NIDDK’s Working Group of Council on Heterogeneity of Diabetes.

Plasma proteome profiling has surged as a promising avenue for biomarker discovery, yet comprehensive platform comparisons remain scarce. Here, the authors evaluate eight proteomics platforms, revealing key differences and complementary strengths, providing crucial insights for researchers into coverage trade-offs and implicating biomarker discovery and clinical applications.

Nature Genetics - The field of blood proteomics faces an upsurge of data with the challenge of cross-study comparisons. This Perspective offers an in-depth analysis and proposes reference materials...

The Qatari sovereign wealth fund adds yet another company to its set of “forward-looking, tech-led” investments. The Qatar Investment Authority (QIA) has invested in a quantum computing company seekin...

Journal of Proteome ResearchDOI: 10.1021/acs.jproteome.5c00287

A neutralizing nanobody specific to the prefusion conformation of herpes simplex virus glycoprotein B has cross-species activity and offers insights into virus neutralization, possible immunogens and ...

Our understanding of the dependence of the genetic and environmental architecture of common diseases on age is incomplete. Here, we use longitudinal data to quantify age-dependent genetic and environm...

Mendelian randomisation analysis of the UK Biobank cohort, supported by analyses of accelerometer-measured activity intensity, reveals that genetically-determined walking pace is associated with longe...

The project, which follows a pilot project profiling 54,000 individuals, will use Olink's Explore HT platform to measure up to 5,400 proteins in 600,000 blood samples.

The MRC Laboratory of Medical Sciences (LMS) is a biomedical research institute where scientists and clinicians collaborate to advance the understanding of biology and its application to medicine. LMS...

First-of-its-kind study will use Seer’s next-generation workflow to identify early-onset cancer biomarkers and power deep, unbiased mass spectrometry-based...

Bioenergetic capacity, the ability to maintain energy balance under stress, can be monitored using blood acylcarnitine profiles. Improving this capacity may slow Alzheimer’s disease progression.

A new era of medical care is upon us.

Author Summary This paper reports what is, to the best of our knowledge, the first genome-wide association (GWA) study with metabolic traits as phenotypic traits. By simultaneous measurements of singl...