iiinova — where your digital self keeps evolving

Reposted by Karl Romanowicz

bioRxiv Synthetic Biology @biorxiv-synthbio.bsky.social · 02/06/2026

DropSynth-Gold: Golden Gate Assembly in Emulsions Extends Multiplexed Gene Libraries to Greater Lengths www.biorxiv.org/content/10.64898/20…

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Reposted by Karl Romanowicz

Calin Plesa @calin.bsky.social · 03/03/2026

New lab preprint: if ML struggles with extrapolation, let's expand the diversity of training data with gene synth, DNA shuffling, and ML gen.... also lots of #FluorescentProteins #ProteinEngineering #MachineLearning #SyntheticBiology 1/n

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Karl Romanowicz @kroman.bsky.social · 11/10/2025

🎥 My Variant Effects Seminar Series (VESS) talk is now on YouTube! I discuss how DropSynth-based gene synthesis reveals how bacterial DHFR variants evolve antibiotic resistance. Thanks to @varianteffect.bsky.social for the invite! 🔗 www.youtube.com/watch?v=KiA9... #VESS #VariantEffects #DropSynth

youtube.com

"Large-scale synthetic gene libraries for decoding variant effects in antibiotic resistance"

YouTube video by Variant Effects

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Reposted by Karl Romanowicz

Atlas of Variant Effects Alliance @varianteffect.bsky.social · 03/09/2025

Next up! Two applications of MAVE! 🧬 DNA damage repair & cancer: Shelby Hemker (@jacobkitzman.bsky.social lab UMich) measures DNA repair of MUTYH variants. 🦠Antibiotic resistance: @kroman.bsky.social (‪@synplexity.bsky.social Univ. of Oregon) decodes how mutations drive resistance in DHFR.

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Karl Romanowicz @kroman.bsky.social · 16/08/2025

Our new Science Advances paper is out! 🎉 The Plesa Lab's first, it builds on DropSynth technology as a proof of concept for large-scale synthetic gene libraries, showcasing a synthetic metagenomics approach to studying antibiotic resistance at scale. 👉 www.science.org/doi/10.1126/...

science.org

Exploring antibiotic resistance in diverse homologs of the dihydrofolate reductase protein family through broad mutational scanning

DropSynth technology enables scalable and cost-effective exploration of antibiotic resistance across the DHFR protein family.

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Reposted by Karl Romanowicz

SynPlexity @synplexity.bsky.social · 26/03/2025

We unlock breakthrough insights by delivering the best training sets in biology—at unprecedented scale. youtu.be/KuCaJTPHM2o

youtu.be

Save 90% on DNA variant libraries today

YouTube video by IndieBio

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Karl Romanowicz @kroman.bsky.social · 19/02/2025

Permafrost thaw takes center stage on Ice & Fire Climate Change podcast! 🎙️❄️🔥 S2 Ep2 "Layer" & Ep3 "Microbes Reawaken" explore carbon release & climate impacts. Hear my research! Tune in: iceandfireclimatepodcast.weebly.com/s2-permafros... #ClimateChange #PermafrostResearch #SciComm

iceandfireclimatepodcast.weebly.com

s2: permafrost thaw

podcast is available for listening on Apple Podcasts, Spotify, Overcast, and through the Public Radio Exchange (PRX) or listen below. Press "More Info" in each episode player to reveal show...

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Reposted by Karl Romanowicz

Calin Plesa @calin.bsky.social · 28/01/2025

New preprint! We carried out the largest complementation and inhibition study for diverse homologs in a single protein family (DHFR) to date. Highlights thread follows... 1/n 🧵

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