Virus-host protein-protein interactions are fundamental to viral pathogenesis, yet few are structurally resolved. Here, the authors combine cross-linking mass spectrometry, bio-orthogonal labeling and...

imageimageThe authors introduce a fused target-decoy strategy and a context-sensitive data subgrouping scheme for cross-linking MS. This strategy allows for the identification of inter-protein links w...

This Article reports cross-linking mass spectrometry (XL-MS) standard datasets with fully controlled protein interactions that are an order of magnitude more complex than existing ones. These datasets...

Advancing data analysis tools for proteome-wide cross-linking mass spectrometry (XL-MS) requires gro