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jsb-lab.bsky.social

@jsb-lab.bsky.social

jsb-lab.bsky.social @jsb-lab.bsky.social · 16/05/2025

Please also check out another exciting work on AI-based protein design, which was a fantastic team effort with Michael Hölzel, @gha14.bsky.social, Tobias Bald and many colleagues at @unibonn.bsky.social www.biorxiv.org/content/10.1...

biorxiv.org

Development of AI-designed protein binders for detection and targeting of cancer cell surface proteins

Artificial intelligence (AI)-based protein design opens new avenues for the rapid generation of new research tools and therapeutics, but experimental validation lags behind the computational design th...

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jsb-lab.bsky.social @jsb-lab.bsky.social · 16/05/2025

We are grateful to the Hans und Ria Messer Stiftung and the cluster of excellence @immunosens.bsky.social for supporting our research at @unibonn.bsky.social and Uniklinikum Bonn

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jsb-lab.bsky.social @jsb-lab.bsky.social · 16/05/2025

This work was led by the phenomenal team of Alexander Hoch, Caroline Fandrey, Thomas Ebert in collaboration with Brian Coventry and David Baker at @uwproteindesign.bsky.social, Tim Kempchen at the Hölzel lab, and Sebastian Virreira Winter and Philipp Geyer from ions.bio

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jsb-lab.bsky.social @jsb-lab.bsky.social · 16/05/2025

Not yet in the preprint: Mass spectrometry confirmed the highly specific degradation of one target protein out of 9,677 human proteins that were quantified in cell lysates

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jsb-lab.bsky.social @jsb-lab.bsky.social · 16/05/2025

Fusing AI-binders to a mutant FKBP12 domain enabled drug-controlled degradation of the endogenous target protein in human macrophages

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jsb-lab.bsky.social @jsb-lab.bsky.social · 16/05/2025

Coupling AI-binders to EGFP enabled the antibody-free staining of an endogenous protein in primary human macrophages

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jsb-lab.bsky.social @jsb-lab.bsky.social · 16/05/2025

Using Transposon-Display, we identified binders for four intracellular human proteins and further improved them by saturation mutagenesis

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jsb-lab.bsky.social @jsb-lab.bsky.social · 16/05/2025

We developed a technology called Transposon-Display, which couples AI binders to their own DNA for pooled screening

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jsb-lab.bsky.social @jsb-lab.bsky.social · 16/05/2025

Can AI-designed binders degrade, aggregate, stain and enrich endogenous proteins in human cells? Check out our new preprint! www.biorxiv.org/content/10.1...

biorxiv.org

Transposon-Display of AI-designed binders enables manipulation of the proteome in human cells

Transposon-Display is a highly scalable screening method that links proteins to their encoding DNA during expression in E. coli via a mutant transposase. Leveraging this system, we identified AI-desig...

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