iiinova — where your digital self keeps evolving

Reposted by Jean-François Trempe

CNPN @cnpn-org.bsky.social · 31/10/2025

Count down for CNPN and HUPO 2025 starte! This is our honor to have our keynote and invited speakers 🤩 @kislingerthomas.bsky.social and Dr. Stuart Cordwell For more info about the program: cnpn.ca/cnpn2025/ #HUPO #proteomics #multi-omics #biomarkerdiscovery #therapeutictargets #bacterialphenotypes

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Reposted by Jean-François Trempe

crbsmcgill.bsky.social @crbsmcgill.bsky.social · 23/04/2025

À ajouter à votre calendrier! Sir Venki Ramakrishnan (MRC-LMB, Cambridge, UK, prix Nobel de chimie 2009) prononcera une allocution au @RIMUHC1 le 1er mai à 17:00 et à l’Hôpital général de Montréal le 2 mai à 11:00. Le programme complet et les modalités d’inscription suivront.

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Reposted by Jean-François Trempe

Harvard Med Cell Biology @harvardcellbio.bsky.social · 11/04/2025

We are so happy for our own Spyros Artavanis-Tsakonas who today was the co-recipient of the Gairdner Award from his work on the Notch pathway! Congratulations!! www.youtube.com/watch?v=hA6d...

youtube.com

2025 Canada Gairdner International Award Drs. Spyros Artavanis-Tsakonas, Iva Greenwald & Gary Struhl

YouTube video by CanadaGairdnerAwards

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Jean-François Trempe @jftrempe.bsky.social · 03/04/2025

Thanks to you and the structural biology platform at @umontreal.ca !

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Reposted by Jean-François Trempe

crbsmcgill.bsky.social @crbsmcgill.bsky.social · 31/03/2025

Tomorrow we host the Annual CRBS Bench-to-Bedside workshop titled "Cellular and Molecular Mechanisms of Aging", with Alanna Watt and Jeremy van Raamsdonk. Please register here: forms.gle/RKy5LxnrguYV.... There will be pizza!

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Reposted by Jean-François Trempe

Bastien Castagner🇨🇦 @castagner.bsky.social · 24/03/2025

We are recruiting! The McGill University Department of Pharmacology and Therapeutics in the Faculty of Medicine and Health Sciences invites applications for a full-time tenure track Assistant or Associate Professor position in the area of toxicology. mcgill.wd3.myworkdayjobs.com/en-US/McGill...

mcgill.wd3.myworkdayjobs.com

Assistant/Associate Professor (Tenure Track), Department of Pharmacology and Therapeutics, Toxicology (L000054)

Please refer to the How to Apply for a Job (for External Candidates) job aid for instructions on how to apply. If you are an active McGill employee (ie: currently in an active contract or position at ...

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Jean-François Trempe @jftrempe.bsky.social · 26/03/2025

Deeply grateful to NSERC for funding this work, as well as the Canada Research Chair program and CFI, which helped create and sustain the SPR-MS Facility and HDX-MS platform. 7/7

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Jean-François Trempe @jftrempe.bsky.social · 26/03/2025

What does it mean for PINK1's function? Mutation of the MPP cleavage site in PINK1 does not affect accumulation on damaged mitochondria, nor processing by PARL under steady-state. However, a.a. 2-34, which binds to MPP, is required for sensing loss of membrane potential. 6/n

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Jean-François Trempe @jftrempe.bsky.social · 26/03/2025

Thus, for MPP to turn over multiple substrates, the alpha and beta subunits must dissociate. Structural modelling shows how MPPalpha binding guides (or misguides in the case of PINK1) the cleavage site of the MTS in the catalytic subunit. 5/n

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Jean-François Trempe @jftrempe.bsky.social · 26/03/2025

Using mass photometry and hydrogen-deuterium exchange mass spectrometry, we show that the PINK1 MTS acts like a molecular glue by interacting extensively with the regulatory MPPalpha subunit and with the catalytic MPPbeta subunit. 4/n

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Jean-François Trempe @jftrempe.bsky.social · 26/03/2025

Intriguingly, we found that while MPP cleaves PINK1's MTS between A28-Y29, it cleaves it much more slowly than other substrates. Using a FRET-based cleavage assay based on MDH2, we find that the PINK1-MTS is a potent (IC50 ca. 500 nM) competitive inhibitor of MPP. 3/n

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Jean-François Trempe @jftrempe.bsky.social · 26/03/2025

We first established a bacterial expression system for human MPP alpha and beta. The enzyme is active in vitro and readily cleaves mitochondrial targeting sequence (MTS) from multiple mitochondrial proteins. 2/n

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Jean-François Trempe @jftrempe.bsky.social · 26/03/2025

Proud to report our latest work on the mechanism of substrate cleavage by the mitochondrial processing peptidase (MPP). Kudos to Andrew Bayne who drove this project, with help from new PhD student Danielle Simons and Naoto Soya in the Lukacs lab. 1/n www.biorxiv.org/content/10.1...

biorxiv.org

Substrate recognition by the human mitochondrial processing peptidase and its processing of PINK1

Nuclear-encoded mitochondrial proteins rely on N-terminal targeting sequences (N-MTS) for their import. These N-MTSs interact with the translocation machinery and are cleaved in the matrix by the mito...

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Reposted by Jean-François Trempe

crbsmcgill.bsky.social @crbsmcgill.bsky.social · 12/03/2025

C’est aujourd’hui #McGill24. ❤️ Donnez maintenant et voyez votre don bonifié, dollar pour dollar. Nous recueillons des fonds pour la bourse en l’honneur de Max Eivaskhani, qui aidera à former de jeunes chercheuses et chercheurs exceptionnels au CRBS. 👇 crowdfunding.mcgill.ca/ui/accueil/p...

crowdfunding.mcgill.ca

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Reposted by Jean-François Trempe

crbsmcgill.bsky.social @crbsmcgill.bsky.social · 12/03/2025

Today is #McGill24. ❤️ Give now and have your gift matched up to dollar for dollar. We are fundraising for the Memorial Studentship in honour of Max Eivaskhani, which will help train outstanding young researchers at McGill CRBS. 👇 crowdfunding.mcgill.ca/ui/main/p/ma...

crowdfunding.mcgill.ca

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Reposted by Jean-François Trempe

Marco A. Maximo Prado @maximoprado.bsky.social · 21/01/2025

Huge impact for prion diseases www.nature.com/articles/s41... In vivo base editing extends lifespan of a humanized mouse model of prion disease

nature.com

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Reposted by Jean-François Trempe

crbsmcgill.bsky.social @crbsmcgill.bsky.social · 21/11/2024

@jftrempe.bsky.social du comité rayonnement du centre a donné une présentation au CEGEP Edouard Montpetit le 30 octobre dernier, devant une salle pleine d'étudiants super allumés! Merci à Carl Ouellet pour l'invitation!

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