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Jamie Hackett @jamiehackett.bsky.social · 19/05/2025

Awhh, my lab are absolute legends!

Jamie Hackett @jamiehackett.bsky.social · 15/05/2025

We are looking to take on an intern at EMBL Rome, for anyone interested in CRISPR and Epigenetics training. The traineeship is 3-12-months, in Rome (Italy), should start by July, and is supervised by the outstanding @steliostsagkris.bsky.social. #job #internship; see info below...

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Jamie Hackett @jamiehackett.bsky.social · 02/12/2024

Postdoc positions in epigenetics! We have several opportunities to investigate big picture questions on (epi)genome function & mechanisms Based @embl.org near Rome (Italy), we offer full scientific support/facilities, training & funding. Also...☀️🏛️🍕🏖️ See👇or contact me for other options. Pls RS

Advert detailing a postdoctoral scientist position at EMBL Rome. Contact: jamie.hackett@embl.it

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Jamie Hackett @jamiehackett.bsky.social · 26/11/2024

Finally, what about aging? Well that certainly has an effect. We detected strong patterns of altered gene expression as early as blastocysts when comparing embryos derived from young vs mature fathers 11/

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Jamie Hackett @jamiehackett.bsky.social · 26/11/2024

Using this metric we found paternal conditioning increase variation in expression across the F1 population. This could be an important phenotypic readout and explain observations of partial penetrance in intergenerational postnatal phenotypes 10/

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Jamie Hackett @jamiehackett.bsky.social · 26/11/2024

Rather than traditional DEG calling - that looks for consistent changes - we also asked whether fathers induce excess variation in expression amongst early embryos 9/

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Jamie Hackett @jamiehackett.bsky.social · 26/11/2024

Pushing further down this line, changing the paternal genetic background completely alters (or ablates) the nature of intergenerational response seen in F1 embryos. This implies a sort of intergenetional GxE (iGxE) interaction that should be further considered 8/

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Jamie Hackett @jamiehackett.bsky.social · 26/11/2024

This effect appears to be robust over independent batches, e.g. Gata4 below (each datapoint = a blastocyst) But! many genes/pathway effects are NOT batch-robust. Thus, multiple batches and independent replicates are necessary to tease out noise/confounders in intergenerational studies 7/

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Jamie Hackett @jamiehackett.bsky.social · 26/11/2024

What is the signature? It seems different expsoures have different effects. Paternal dybiosis by antibiotics (nABX) appears to affect lineage marker expression and/or allocation of the primitive endoderm lineage relative to epiblast lineage This is not seen in paternal LPHS embryos 6/

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Jamie Hackett @jamiehackett.bsky.social · 26/11/2024

Indeed, using many replicates/batches, we detected induction of a clear gene expression signature in blastocysts, depending on paternal conditioning 5/

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Jamie Hackett @jamiehackett.bsky.social · 26/11/2024

Clustering on transcriptome showed no separation. Not surprising since these embryos should all develop into relatively normal pups BUT, there was tendency for embryos to sit at opposite ends of the PC1 spectrum if their father was dysbiotic (nABX) vs on High-sugar (LPHS) diet. See histogram 4/

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Jamie Hackett @jamiehackett.bsky.social · 26/11/2024

We used IVF at scale, with fathers exposed to gut dysbiosis, altered diets, or control used as sperm donors. We then generated single-embryo transcriptomes from multiple batches and >800 embryos across the study 3/

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Jamie Hackett @jamiehackett.bsky.social · 19/11/2024

Back at the EMBL mothership this week for the Quantitative Biology symposium Looks a cracking speaker lineup and set of posters on diverse topics… and it’s definitely fresh in Heidelberg 🥶 www.embl.org/about/info/c...

Pic of entrance to the EMBL advanced training centre

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Jamie Hackett @jamiehackett.bsky.social · 15/11/2024

Fantastically engaging talk on #microbiome -brain axis from @jfcryan.bsky.social in Rome this morning. Full of thought-provoking ideas and some sage advice: “If you’re thinking of having a midlife crises… forget about the motorbikes and think about leeks instead” 😂

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