The molecular factors and mechanisms that mediate the chromatin recruitment of activation-induced deaminase and the generation of its stalled polymerase II substrate during somatic hypermutation are i...

Increased RNA polymerase III (Pol III) activity and tRNA abundance are widely linked to cancer cell growth, yet the functional requirement for individual Pol III genes and core components remains uncl...

Understanding how cells make decisions over time requires the ability to link past molecular states to future phenotypic outcomes. We present TimeVault, a genetically encoded system that records and s...

Single-molecule fluorescence microscopy studies by Zhou et al. show that transcription activator proteins bind at UAS/enhancer DNA and then sequentially or simultaneously recruit Mediator coactivator and RNA polymerase II to form an initiation complex precursor. Multiple DNA-bound activators synergistically enhance recruitment by binding to the same Mediator complex.

Gene expression is regulated by the targeted degradation of transcription factors through the ubiquitin-proteasome system. Transcription factors destined for degradation are recognized by E3 ubiquitin...

The neuron-specific RNA binding protein NOVA1 has a single amino acid substitution unique to modern humans. Here, the authors characterize the evolutional specificity and the function of the substitut...

The snRNA Activating Protein Complex (SNAPc) mediates snRNA transcription by RNA Pol II and III. Here, the authors have used cryo-EM to resolve the structure of the SNAPc-bound RNA Pol III pre-initiat...