📄 New protocol chapter! Colyer, Donaldson, Banda, Bennett & Mehra describe five agar-based systems for studying how roots sense patchy water: hydrotropism, xerotropism, xerobranching & hydropatterning. For Arabidopsis & crops 🌱
Check the protocol: doi.org/10.1007/978-...
![Image: Description and validation of pMDS plasmid system for dual analysis of transcription and translation in plants. (A) Organization of pMDS1 vector showing reporters for transcription [mTurquoise (mTurQ)], translation (C-terminal mVenus), and a 2A self-cleaving peptide. (B) Organization of pMDS2 vector showing reporters for transcription (mTurQ), translation (N-terminal mVenus), and a 2A self-cleaving peptide. (C) Confocal image of pMDS1_SHRpro:SHR:mVenus:mTurQ showing gene expression (mTurQ) in the stele region and protein (mVenus) translocating to endodermis in the root meristem. (D) Confocal image of pMDS2_VAM3pro:mVenus:VAM3:mTurQ showing subcellular expression (mTurQ) in the nucleus and protein (mVenus) moving to the vacuole in the root epidermis. Red channel shows mCherry expression. nu, nucleus; vac, vacuole; *, endodermis of root meristem. Scale bar, 10 μM. Credit: Science Advances](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:5l42c4gpfv34lqzigeftvql4/bafkreia57owau5zyahyrvj5c4vzu3edakjcabo664cbauhnkhvskrw66ai)
