Stem cell niches are complex multi-signalling networks comprised of molecular cues and physical interactions, orchestrated by niche-resident cells and the extracellular factors they produce. The bone....

Extracellular vesicles (EVs) are nano-sized membrane-bound structures thought to be secreted by all cells and increasingly recognized as key mediators of intercellular communication. Established EV isolation protocols for bodily fluids-primarily focus on blood with limited insights into methods optimized for EVs from other hematopoietic regions. In this study, we present a novel protocol for the isolation and enrichment of EVs from human trabecular bone and bone marrow. This method employs a two-step purification strategy, combining iodixanol density cushion (IDC) ultracentrifugation with size exclusion chromatography (SEC), and enables EV recovery from fresh tissue hours after collection. Importantly, this approach facilitates the enrichment of bone-derived EVs without the need for enzymatic digestion or long-term culture, preserving native EV populations. This protocol offers a valuable tool for researchers investigating EVs derived from the diverse cellular constituents of the bone microenvironment. ### Competing Interest Statement The authors have declared no competing interest. Canadian Institutes of Health Research, https://ror.org/01gavpb45, 394568 Terry Fox Research Institute, https://ror.org/00mtf6c40, 1133

Hematopoietic stem cells (HSCs) acquire somatic mutations throughout life, with the frequency of mutational incidence increasing with age [1]. Occasionally, certain mutations confer a fitness advantag...

Isolating and studying haematopoietic stem cells in young and aged mice demonstrates evolutionary processes related to blood production and provides a framework for interpreting future work using labo...

Jeppesen, Sanchez et al. identify blebbisomes as large membrane-enclosed vesicles containing organelles and secreted by cells. Blebbisomes can both secrete and internalize exosomes and microvesicles.

Conventional microscopy techniques are limited by a resolution limit of 250 nm, making it challenging to visualize subcompartment structures. Single molecule localization microscopy (SMLM) has revolu...

Doctors and researchers in Kingston as well as cancer clinics and labs across the country will spend the next year following “patients’ orders” rather than the other way around after receiving funds f...

Bridge to Faculty Postdoctoral Research Associate in Microbiology and ImmunologyThe Department of Microbiology and Immunology (MI) at the University o...

Abstract. Ribosome profiling (Ribo-Seq) has revolutionised our understanding of translation, but the increasing complexity and volume of Ribo-Seq data pres

Understanding how cell intrinsic and extrinsic factors combine to initiate transformation holds promise for the development of strategies to prevent, detect and treat cancer early. In this Review, Jas...