dlvr.it
Two-photon voltage imaging with rhodopsin-based sensors
Rhodopsin-based GEVIs were thought to lose voltage sensitivity with two-photon excitation, limiting their utility for deep in vivo imaging. We introduce Jarvis, a bright and photostable GEVI, and demonstrate that, along with pAce and Voltron2, it enables high-contrast two-photon voltage imaging and AP detection at kilohertz rates.