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Fabrizio Casilli @fabricas.bsky.social · 11/11/2025

Last week I have defended my PhD in the beautiful Aula of the @rug.nl with a thesis entitled "Mapping the Sequence Landscape of an Artificial Enzyme". Such a huge diploma is proportionate to the immensely beautiful experience behind a PhD in the @roelfesgroup.bsky.social. Thank you very much to all!

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Reposted by Fabrizio Casilli

Steph @stephy.bsky.social · 18/09/2025

Merck is accepting applications for its 2026 Future Talent Program - these are internships and co-ops in a variety of areas including drug discovery, vaccines, clinical trials, biostatistics, epidemiology, HEOR, data science, regulatory affairs, and more #chemsky 🧪⚗️ jobs.merck.com/us/en/search...

jobs.merck.com

Your job search results | Merck Careers

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Reposted by Fabrizio Casilli

Aesthetics & Learning Lab (ALL) @allab-iwm.bsky.social · 18/08/2025

Hi Bluesky! 🦋 We are the Aesthetics and Learning Lab (ALL). ALLab is based in the Leibniz-Institut für Wissensmedien (IWM) in Tübingen. Its aim is to empirically investigate how and what can be learned from aesthetic experiences ✨ Stay tuned, for now... That's ALL folks!

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Fabrizio Casilli @fabricas.bsky.social · 08/08/2025

👨‍💻👨‍🔬 This study shows that the collaboration of dry and wet lab is fundamental to achieve the improvement so sought in biocatalysis. A big thank you goes to the first author Mahdi, and to all the collaborators at Zymvol that participated in this project.

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Fabrizio Casilli @fabricas.bsky.social · 08/08/2025

🧪 Demonstrated on an engineered artificial enzyme based on the Lactococcal multidrug resistance regulator (LmrR) scaffold, the study reveals how distant residues communicate with catalytic regions and their mutation can fine-tune this communication affecting functional properties.

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Fabrizio Casilli @fabricas.bsky.social · 08/08/2025

🔗 The pipeline allows to identify distal mutation hotspots—sites distant from the active site, that can still influence enzyme functional properties (e.g. activity and thermostability)

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Fabrizio Casilli @fabricas.bsky.social · 08/08/2025

💻 We presented an open‑source computational workflow integrating residue network analysis, allosteric pathway mapping, and a ML-based functional site prediction model.

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Fabrizio Casilli @fabricas.bsky.social · 08/08/2025

📢📢 New paper out! AI and networks can be used to predict distal mutations in proteins, even in artificial enzymes! Check out the article here ----‐> doi.org/10.1016/j.ij... Thread 1/5 🧵

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Fabrizio Casilli @fabricas.bsky.social · 04/07/2025

🌍 This work is the result of a great collaboration between the @roelfesgroup.bsky.social (@stratinghinst.bsky.social) at the @rug.nl and the NMR team at the @utrechtuniversity.bsky.social. Great work everybody! 5/5

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Fabrizio Casilli @fabricas.bsky.social · 04/07/2025

💡 We found that the mutations significantly modify the dynamics of the whole protein, promoting a monomeric state (instead of the usual dimer structure) and allowing a novel interaction with the reaction substrates. 4/5

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Fabrizio Casilli @fabricas.bsky.social · 04/07/2025

🧪 The wise use of a diamagnetic analogue (Zinc containing) of the cofactor allowed to track the changes in the structure of the enzyme in apo- and bound- state to the cofactor, as well as to the reaction substrates. 3/5

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Fabrizio Casilli @fabricas.bsky.social · 04/07/2025

🔬 We used NMR to rationalize the role of two mutations that increase the catalytic activity towards the Friedel-Crafts Alkylation of indoles in an artificial metalloenzyme. The catalyst is based on a copper-phenanthroline complex bound within the hydrophobic pocket of the versatile LmrR protein. 2/5

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Fabrizio Casilli @fabricas.bsky.social · 04/07/2025

📢 New publication alert 📢 🧪Studying enzyme conformational dynamics always hides new surprises, especially when you discover that beneficial mutations destabilize the enzyme! Read more here ---> doi.org/10.1002/cbic... Thread 🧵 1/5

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Fabrizio Casilli @fabricas.bsky.social · 19/05/2025

Still better than default MS excel settings 🤭

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Reposted by Fabrizio Casilli

Roelfes Group @roelfesgroup.bsky.social · 12/05/2025

📢📢📢 PhD Opportunity! Join our research group at RUG! We're seeking a motivated PhD student for a project on biocatalytic cascade processes combining natural and designer enzymes, supported by machine learning. Click below to learn more — and feel free to share! sites.google.com/rug.nl/roelf...

sites.google.com

Roelfes group - Open Positions

PhD position on Hybrid biocatalytic cascades aided by Machine learning (1.0 FTE)

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Reposted by Fabrizio Casilli

Roelfes Group @roelfesgroup.bsky.social · 14/02/2025

Our latest publication is out in @angewandtechemie.bsky.social! We generated and engineered a novel artificial metalloenzyme for the enantioselective synthesis of a panel of γ-nitro-carbonyl compounds on a preparative scale.

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Reposted by Fabrizio Casilli

Roelfes Group @roelfesgroup.bsky.social · 23/12/2024

Season's greetings from Roelfes and Mayer groups! Wishing our colleagues, collaborators, and the entire community a joyful holiday season and a prosperous New Year ahead! 🎉🎄✨🧪

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Fabrizio Casilli @fabricas.bsky.social · 06/12/2024

Two days of very interesting talks at #NWOChains24, big thanks to everyone that stopped by my poster for the stimulating exchange of ideas. Happy to have won the poster prize! 🏆🏆

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Reposted by Fabrizio Casilli

Charlie Harris @harrisbio.bsky.social · 21/11/2024

Now I can share external links without the posts being down regulated - I thought it would share this again!:) I have compiled a list of now 100+ companies in the 'TechBio' space into a fully open database for the community. Find here and please share if you like open.substack.com/pub/harrisbi...

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