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Evdokiia Potolitsyna

@evdokiiap.bsky.social
39 followers 75 following 11 posts

DStrain MSCA fellow at University of Oslo Previously at Baylor College of Medicine

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Reposted by Evdokiia Potolitsyna
EMBL @embl.org · 25/09/2026
🔬 A new study by EMBL researchers, published in @science.org, about the pre-ribosome assembly pathways inside intact human cells, reveals — at molecular resolution — in-cell structural snapshots of both ribosomal subunit precursors, the SSU processome and the pre-60S, and novel configurations.
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Reposted by Evdokiia Potolitsyna
Karsten Rippe @karsten-rippe.bsky.social · 09/09/2026
🧬 1/ HP1α marks silenced heterochromatin, but how does it repress, and what happens when transcription is switched on? On mouse fibroblast chromocenters, HP1α stays bound, its barrier is local, and the domain reorganizes nanodomain by nanodomain. 👉 @cp-molcell.bsky.social doi.org/10.1016/j.mo...
Schematic of the two experimental systems. Left, activators recruited to chromocenters of wild-type and Suv39h dn mouse fibroblasts, with DAPI and anti-HP1α images showing HP1α enrichment and loss. Right, the U2OS 2-6-3 reporter array with an activator recruited alone or together with HP1α.
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 26/08/2026
9/ Thank you to everyone in #Ribacklab and to our amazing collaborators! @superscijew.bsky.social @sciencyelmira.bsky.social @sthennigan.bsky.social
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 26/08/2026
8/ More broadly, our findings support a role for phase separation not only in compartmentalizing biochemical reactions, but also in tuning their output to physiological demand.
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 26/08/2026
7/ Together with our collaborators, we further confirm that similar phenotypes are found in mouse stem cells and even in plant leaves upon energy depletion, suggesting a conserved response. @luciastrader.bsky.social, @goodell-lab.bsky.social
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 26/08/2026
6/ Is nucleolar hibernation a terminally aberrant state? Surprisingly, when we replenish nutrients, nucleolar properties recover within minutes. This suggests hibernation is an adaptive state that poises the nucleolus for rapid restart.
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 26/08/2026
5/ What is nucleolar hibernation? Using several approaches, including tracking MS2-tagged rRNA in live cells, we find that hibernating nucleoli have restricted rRNA transport: rRNA diffuses less, and the nucleolus is more viscous, consistent with NPM1 stabilization.
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 26/08/2026
4/ What does this imply for ribosome biogenesis? We find that while transcription of new rRNA is decreased, rRNA intermediates remain “stuck” in the nucleolus. The combination of a stabilized granular component and retained rRNA intermediates led us to call this state nucleolar hibernation.
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 26/08/2026
3/ Using live-cell imaging of endogenously tagged NPM1, we find that nutrient availability tunes its nucleolar interaction strength, with partitioning changing by ~2-fold. Surprisingly, nutrient deprivation enriches NPM1 in the nucleolus, in contrast to immunofluorescence.
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 26/08/2026
2/ Previous studies find that the nucleolus correlates well with ribosome demand, with lower demand meaning smaller nucleoli. Phase separation allows more possibilities - we searched for new axes of nucleolar control in coordination of ribosome biogenesis.
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 26/08/2026
1/ Do cells use phase separation to adapt biosynthetic output to changing demands? In our new preprint, we show that under nutrient limitation, the nucleolus enters a reversible ‘hibernation’ state that slows ribosome biogenesis. See doi.org/10.64898/202...
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Reposted by Evdokiia Potolitsyna
bioRxiv Cell Biology @biorxiv-cellbio.bsky.social · 02/06/2026
In-cell structures visualize human pre-ribosome assembly in the nucleolus www.biorxiv.org/content/10.64898/20…
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Reposted by Evdokiia Potolitsyna
Anders Sejr Hansen @andersshansen.bsky.social · 05/05/2026
(1/n) Super excited to share that our preprint is out today in @natsmb.nature.com with a new name "Integrated MINFLUX tracking reveals two distinct chromatin dynamics classes across cell types" and more than 2x more data: www.nature.com/articles/s41... See also MIT News news.mit.edu/2026/how-chr...
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Reposted by Evdokiia Potolitsyna
Sinem Saka @sinemsaka.bsky.social · 20/03/2026
Spectral multiplexing is typically limited to 4-5 channels. Our new preprint introduces a framework that utilizes DNA barcoding and signal tuning to enable robust spectral unmixing and ground-truth benchmarking to achieve 15-plex subcellular profiling without cycling www.biorxiv.org/content/10.6...
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Reposted by Evdokiia Potolitsyna
Tugce Aktas @aktast.bsky.social · 25/02/2026
Our most recent work on the “function and evolution” of #nuclear-speckles is now online at Cell @cp-cell.bsky.social doi.org/10.1016/j.ce... Read the thread👇 for the highlights of our findings.
doi.org
Redirecting
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Reposted by Evdokiia Potolitsyna
Stella Hurtley @smhsci.bsky.social · 20/02/2026
Out now in @science.org Ribosomal RNA expansion segments mediate the oligomerization of inactive animal ribosomes | Science www.science.org/doi/10.1126/...
science.org
Ribosomal RNA expansion segments mediate the oligomerization of inactive animal ribosomes
Cells down-regulate protein synthesis when stressed to conserve energy and shift resources toward repair. We found that in some mammalian cells, including neurons, stress also resulted in the formatio...
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 08/12/2025
Last week marked the end of my postdoc at #RibackLab. I spent three exciting and productive years at BCM, but it’s now time for the next chapter. Thank you to @superscijew.bsky.social for being a great mentor and to my colleagues for all the fun and science we shared together!
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Reposted by Evdokiia Potolitsyna
Polina Tikanova @polinatikanova.bsky.social · 24/11/2025
Happy to share that my PhD project is finally published!🪱✨ Selfish genes are found across the tree of life. They can disrupt inheritance patterns and at the same time act as units for molecular innovation. Here we tried to answer one big question: how do selfish genes emerge in the first place?
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Reposted by Evdokiia Potolitsyna
Josh Riback @superscijew.bsky.social · 04/11/2025
(1/10) How do diverse leukemia mutations converge on the same molecular program? In #RibackLab first manuscript @cp-cell.bsky.social, collaboration with @goodell-lab.bsky.social shows that disparate mutations rewire shared protein networks to form nuclear condensates called C-bodies.
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Evdokiia Potolitsyna @evdokiiap.bsky.social · 28/02/2025
Our lab's first preprint is out! I'm glad to be part of this project - take a look!
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