Ageing reflects progressive decanalization of mammalian cell identity, driven by erosion of a regulatory grammar written during development.

By being restricted to target regions by MLLT1–MLLT3 occupancy, activation-induced deaminase activity is able to avoid most off-target mutagenesis during B cell somatic hypermutation.

In this Review, Wu, Rashid et al. discuss how mechanical signals regulate the ability of cells to facilitate and maintain changes in cell memory. They exemplify these effects in cellular structure rem...

Triple-negative breast cancer (TNBC) is highly heterogeneous, complicating treatment. Here, the authors show that the H4K20me3 heterochromatin mark regulates lineage identity and the unfolded protein ...

Tracking the biological age of grafted organs suggests that donor pools could be expanded to boost organ availability.

This study uses single-cell ATAC–seq to map chromatin accessibility in the intestinal mucosa from patients with Crohn’s disease and healthy controls to characterize regulatory programs associated with...

Simulations integrating micro-C and imaging data provide a coherent view of chromatin organization from nucleosomes to clutches to domains, revealing that most regions form compact domains but short r...

A photolabile oligonucleotide system enables spatially resolved single-cell measurements to show how the epigenome regulates gene expression.

Researchers show that the zoonotic parasite Toxoplasma gondii built a unique chromatin modifying complex to activate the genes that drive infection and virulence, revealing a potential weak spot for n...

Ji et al. show that radiotherapy induces a TET2-dependent epitranscriptomic program in myeloid cells that promotes immunosuppressive M-MDSC differentiation. TET2 loss preserves caRNA m5C-MBD6 signaling, restores IRF3-IFN-I activation, generates myeloid-derived activating cells, and enhances radiotherapy-induced antitumor immunity.

In this Review, Buckley et al. discuss how DNA methylation is interpreted in mammals and plants, focusing on the reader proteins and transcription factors that bind to methylated DNA and the downstrea...

Viral mimicry is the activation of antiviral immune responses by endogenous nucleic acids that have accumulated in the cytosol. In this Review, Chen et al. highlight how cancer-associated epigene...

The Rad6B–Tex1 ubiquitination complex drives malaria parasite sexual commitment by monoubiquitinating histone H2B, and thereby enabling gametocyte formation and mosquito transmission.

The authors identified and engineered a miniature, innate nuclease-dead xCas12m enabling effective epigenome editing. xCas12m–CRISPRoff achieved durable epigenetic silencing and inhibition of hepatitis B virus infection through single-AAV delivery.

Single-molecule genomics methods are used to study the activity of regulatory factors on individual DNA molecules genome-wide, thereby enabling quantification of molecular heterogeneity and co-occurre...