Devine lab @devinelab.bsky.social · 24/08/2026Here we describe step-by-step how to visualise dopaminergic release in acute brain slices, using the fluorescent false neurotransmitter 200 (FFN200). This protocol can be used with other FFNs, and explains steps from imaging🔬 to data analysis✅ 081
Devine lab @devinelab.bsky.social · 24/08/2026We are excited to share a new paper from our lab presenting a protocol developed by Patrick Cottilli during his PhD placement at Sulzer lab in Columbia University, NY www.cell.com/star-protoco...cell.comProtocol for fluorescent false neurotransmitter live imaging of dopamine release dynamics from individual synapses in acute brain slicesStriatal dopaminergic (DA) axons have many en passant boutons with synaptic vesicles, but only a fraction display exocytosis. Here, we present a protocol for live imaging of murine DA axons using fluo... 040
Reposted by Devine labAli Twelvetrees @dozenoaks.bsky.social · 09/03/2026Save the date for #NeuroBioUK26 Do you like neurons? Maybe glia? Molecules? Cell biology? - The venue is booked and we are excited to see you and your incredible science there on the 11th of September! Please do join our email list to stay up to date. neurobiouk.sites.sheffield.ac.uk 02212
Devine lab @devinelab.bsky.social · 13/03/2026⚡️We are excited to share a new paper from the lab by @drkhole.bsky.social in @cp-cellrepmethods.bsky.social - suggesting MitoTracker dyes are not a reliable tool for detecting intercellular mitochondrial transfer❗️ www.cell.com/cell-reports...cell.comMitoTracker transfers from astrocytes to neurons independently of mitochondriaThe mitochondrial dye MitoTracker is commonly used to investigate intercellular mitochondrial transfer (IMT), particularly between astrocytes and neurons. Hole et al. compare MitoTracker with a geneti... 14224
Devine lab @devinelab.bsky.social · 21/11/2025Check out the first review from the Mitochondrial Neurobiology lab on... lysosomes! Learn about the non-canonical roles of lysosomes in neurons from @jonnyspencer.bsky.social @ysudarikova.bsky.social: www.cell.com/trends/neuro...cell.comNon-canonical roles of lysosomes in neuronsNeurons are highly polarised and compartmentalised cells with organelles that are specialised to support their spatial and functional demands. This includes lysosomes, which are single-membrane-bound ... 1144
Devine lab @devinelab.bsky.social · 20/11/2025Congratulations to Dr. Patrick Cottilli who has successfully defended his PhD viva yesterday!!🎉 This is a huge milestone for our lab - our first-ever PhD graduate!⭐ So proud of your hard work, creativity, and perseverance throughout these 4 years. Here's to the amazing science ahead!🔬 052
Reposted by Devine labRupert Beale @bealelab.bsky.social · 10/10/2025Do you want to do a PhD with me and Mike Devine? Of course you do! Or if not, maybe you know someone who does? @devinelab.bsky.social www.crick.ac.uk/careers-stud...crick.ac.ukBeale & Devine Lab | Role of the V1H subunit of the V-ATPase in neurons and cell-autonomous immune responses 056
Devine lab @devinelab.bsky.social · 05/06/2025We are excited to share a new preprint from our lab led by Katy Hole (@drkhole.bsky.social) in collaboration with MSD (UK). 160