Chiara Mazzoni @chimazz.bsky.social · 03/04/20264/4 Different gut microbial species are either lost or expanded in IBD, and the decrease in species count is not dependent on the higher human fraction. In CD, species richness is particularly stunted in adults and seems to continuously decline with the years. 110
Chiara Mazzoni @chimazz.bsky.social · 03/04/20263/4 We trained a set of XGBoost models on publicly available adult IBD metagenomes and then tested on our pediatric ones. Human reads % is the most important feature across models and training on microbial species presence-absence or relative abundance is virtually the same! 110
Chiara Mazzoni @chimazz.bsky.social · 03/04/20262/4 Fecal human DNA % increases with disease severity, but clinical treatment can determine large variance within study groups. Focusing on the ratio between neutrophils and epithelial cells allows us to distinguish between remissive and active IBD. 110
Chiara Mazzoni @chimazz.bsky.social · 03/04/20261/4 Using ddPCR and methylation marker profiling, we found that in IBD patients the majority of fecal human DNA is neutrophilic, dominating over colonic and intestinal fractions. If you have only shotgun metagenomics, human reads % is a good approx. for neutrophil DNA. 110
Chiara Mazzoni @chimazz.bsky.social · 12/06/2025Embedding your [personality] in the latent space of your [one-slide presentation] #TCTeAC (cit. @yanabromberg.bsky.social ) 171
Chiara Mazzoni @chimazz.bsky.social · 12/06/2025Science culture #TCTeAC (sigh, ChatGPT could not put the tape on the hole) 051