Reposted by Cansu Dinçer
Excited to see BEstimate out -
a computational tool for base editing guide RNA design for screening, disease modelling and correction of pathogenic variants.
It's SNP-aware, so you can parse non-reference genomes, and reports off-target sites.
paper:
link.springer.com/article/10.1...
link.springer.com
BEstimate: a computational tool for the design and interpretation of CRISPR base editing experiments - Genome Biology
CRISPR base editors enable scalable targeted DNA mutagenesis and are a powerful tool for analysing the function of variants of uncertain significance and disease modelling. Existing guide RNA (gRNA) d...
