iiinova — where your digital self keeps evolving

Bart Brouwer @brouwerb.bsky.social · 30/04/2026

Proud to share that another one of my main PhD projects has now been published! The manuscript entitled "Exploring PadR Proteins for Artificial Enzyme Design" is now available online in #ChemBioChem. Keep readin below or check out the full paper here: doi.org/10.1002/cbic... Thread ⬇️

doi.org

Exploring PadR Proteins for Artificial Enzyme Design

Inspired by the Lactococcal multidrug resistance regulator (LmrR) protein scaffold, new phenolic acid decarboxylase transcriptional regulator (PadR) proteins were identified and evaluated as scaffold...

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Reposted by Bart Brouwer

Max Fürst @maxfus.bsky.social · 20/05/2025

🚨 preprint 2️⃣ this month: our (purely experimental🧪) venture into #ChemBio We prouldy present: ADD-tagging of proteins (or "ADDing") —a super convenient enzymatic technique to install click chemistry handles on proteins. Led by superstar @wahyuwidodo.bsky.social www.biorxiv.org/content/10.1... A 🧵👇🏽

Overview of ADD-tagging including ADP-ribosyl cyclase (ADPRC)-catalysed dinucleotide substrate generation and two step chemoenzymatic labelling of target proteins with the flavin transferase ApbE, followed by click chemistry-based functional group attachment.

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Reposted by Bart Brouwer

Roelfes Group @roelfesgroup.bsky.social · 12/05/2025

📢📢📢 PhD Opportunity! Join our research group at RUG! We're seeking a motivated PhD student for a project on biocatalytic cascade processes combining natural and designer enzymes, supported by machine learning. Click below to learn more — and feel free to share! sites.google.com/rug.nl/roelf...

sites.google.com

Roelfes group - Open Positions

PhD position on Hybrid biocatalytic cascades aided by Machine learning (1.0 FTE)

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Bart Brouwer @brouwerb.bsky.social · 23/04/2025

Very proud to share that one of my main PhD projects has been published in @chemicalscience.rsc.org, and that it has been selected as a #ChemSciPicks of the week paper! Keep reading below for more details, or check out the full paper for free online: doi.org/10.1039/D5SC... Thread ⬇️

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Reposted by Bart Brouwer

Franco Della Felice @francdefel.bsky.social · 12/02/2025

Grateful to be part of this awesome project lead by @brouwerb.bsky.social. Take a look to our preprint about unlocking iminium ion catalysis when using amino-tyrosine as a genetically encoded amino acid! 🤓 #MSCA @roelfesgroup.bsky.social

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Bart Brouwer @brouwerb.bsky.social · 11/02/2025

Check out our latest preprint, in which we demonstrate the first example of using noncanonical 3-aminotyrosine (aY) as a catalytic residue for iminium activation in a designer enzyme! Thread ⬇️

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